FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004717566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278538
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCCTCGGCGCCCAGGCTGTTCATTTCCAGATACAGGGTGTTCATGGAATTGTCTCTGG12370.4441045746002341No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC7380.26495487150765784No Hit
ATATTACTGTGCGAGAGGGGGCTATTTTGATCTTTTGAGTGGTTTTCATCTTATGGACGTCTGGGGCCAAGGGAC6910.24808105177749534No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGTGCTGCTACTTGTATATGAGCTGCAGTAATAAT6840.24556792969002433No Hit
CAGTAATACTGGTCGTCTCACATACTACGCAGACTCCGTGAAGGGCCGGT6830.24520891224895708No Hit
GGACTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCC6630.2380285634276113No Hit
CTATATTACTGTGCGAGAGGGGGCTATTTTGATCTTTTGAGTGGTTTTCATCTTATGGACGTCTGGGGCCAAGGG6420.23048919716519828No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT5750.20643502861369006No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTG5620.20176780187981533No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT5600.20104976699768076No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA5430.19494647049953687No Hit
CAGTAATATAGGGCCGTGTCCTCGGCGCCCAGGCTGTTCATTTCCAGATA5400.19386941817633502No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5300.19027924376566213No Hit
GATTATTACTGCAGCTCATATACAAGTAGCAGCACCGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT5160.1852529995907201No Hit
GTATTACTGATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATCGCTGCTA4960.1780726507693744No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATC4450.15976276127494274No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT4450.15976276127494274No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTCGTTATAACTATGTCTCCTGGTTCCAACAACGCCCAGGCAAAGC4390.157608656628539No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC4360.15653160430533716No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC4270.15330044733573156No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT4230.1518643775714624No Hit
ATATAGGGCCGTGTCCTCGGCGCCCAGGCTGTTCATTTCCAGATACAGGG4080.1464791159554531No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC4070.14612009851438584No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGTGCTGCTACTTGTATATGAGCTG4020.1443250113090494No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG3920.14073483689837654No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG3910.14037581945730923No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC3870.13893974969304007No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTCGTTATAACTATGTCTCCTGGTTCCAACAACG3820.13714466248770366No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT3800.13642662760556906No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT3790.13606761016450178No Hit
GACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCT3710.13319547063596351No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC3590.12888726134315603No Hit
GCCCTATATTACTGTGCGAGAGGGGGCTATTTTGATCTTTTGAGTGGTTTTCATCTTATGGACGTCTGGGGCCA3560.1278102090199542No Hit
CCCCTAAGGGCCGATGAATGACATCATAAATCATGAGTGTGGGGGCTTTGCCTGGGCGTTGTTGGAACCAGG3470.12457905205034861No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC3440.12350199972714675No Hit
TTATAACTATGTCTCCTGGTTCCAACAACGCCCAGGCAAAGCCCCCACAC3430.12314298228607945No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATCGCTGCTAAAGGTGGATCCAGAAGTTACAC3410.1224249474039449No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC3390.12170691252181032No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC3370.12098887763967574No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC3360.12062986019860844No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAAGATGAAAAC3340.11991182531647389No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG3330.11955280787540659No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC3240.116321650905801No Hit
GATCAGGACTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGC3220.11560361602366642No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAAC3190.11452656370046457No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA3170.11380852881833No Hit
TAGTTATAACGACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT3160.11344951137726271No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA3140.11273147649512813No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG3130.11237245905406083No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC3120.11201344161299356No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG3110.11165442417192628No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC3050.10950031952552254No Hit
GGCAAAGCCCCCACACTCATGATTTATGATGTCATTCATCGGCCCTTAGGGGTTTCTAACCGCTTCTCTGGCTCC3020.10842326720232069No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT3010.1080642497612534No Hit
GTGCTGCTACTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC3000.10770523232018611No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC2990.10734621487911881No Hit
GTATGTGAGACGACCAGTATTACTGATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT2960.10626916255591698No Hit
CTCCTGTGTAACTTCTGGATCCACCTTTAGCAGCGATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG2920.10483309279164782No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACC2900.10411505790951325No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG2900.10411505790951325No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTG2880.10339702302737867No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG2870.10303800558631139No Hit
GAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT2850.10231997070417681No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC2830.10160193582204223No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC2820.10124291838097496No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG2810.10088390093990766No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA2800.10052488349884038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)