FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552410
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20990.3799713980557919No Hit
GTAATATATGGCCGTGTCCTCGACTCTCAGGCTACTCATTTGCAGATATACCGTATTCTTGGAATTGTCTCTGG20760.3758078238989157No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT19430.3517315037743705No Hit
GTTCTGGGACAGACTTTAGTGTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACATATTACTGTCAACAAT17850.3231295595662642No Hit
ATATATTACTGTGCGAAAGATATGTTGTATGATCTCCCTGAATATTCGTC15770.28547636719103564No Hit
GTCCCAACCAGGACACAGCCTGGACATGAGGGTCCCTGCTCAACTCCTGG15060.27262359479372206No Hit
GACCTGGGAGAGCCCCTAAACTCCTGATCTACGAAACATCCAAGTTGGAA14630.26483952136999694No Hit
ATATTACTGTCAACAATATGATGAGGTTCCGCTCACTTTCGGCGGAGGGA14400.26067594721312065No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13990.2532539237160804No Hit
GTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGTAAGTCTTTAAATTGGTATCAGCAG13340.24148730109882152No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC13090.2369616770152604No Hit
GTCCCAGAACCACTCCCACTGATCCTCGATGGGACCCCTACTTCCAACTTGGATGTTTCGTAGATCAGGAGTTT12690.2297206784815626No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG12300.22266070491120726No Hit
CTCCTGTACAGTGTCCGGATTCGTCTTCGGCAACTTTGCCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGACT12260.22193660505783747No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12160.22012635542441303No Hit
GGACTGAACAGGGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT11930.2159627812675368No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11380.2060064082837023No Hit
GACTTTAGTGTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACATATTACTGTCAACAATATGATGAGGTT11290.2043771836136203No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT11260.20383410872359298No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11200.20274795894353834No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG11170.20220488405351097No Hit
GGACTAGAGTGGGTCTCAAGGATTAATCGAAGTGGTGGAAGGGCATCCTACTCAGGCTCCGTGAGGGGCCGTTTC11120.20129975923679874No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACA10870.19677413515323763No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGTA10860.19659311018989517No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10570.19134338625296426No Hit
CACTAAAGTCTGTCCCAGAACCACTCCCACTGATCCTCGATGGGACCCCTACTTCCAACTTGGATGTTTCGT10410.18844698683948516No Hit
ACATATTACTGTCAACAATATGATGAGGTTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAGGA10320.18681776216940316No Hit
GGACATGAGGGTCCCTGCTCAACTCCTGGGGCTCCTGCTGCTCTGGCTCT10290.18627468727937582No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGACACTAAAGTCTGTCCCAGAACCACTCCC10180.18428341268260895No Hit
GCCTGGACATGAGGGTCCCTGCTCAACTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGAGGTGACATCC10060.18211111312249958No Hit
AACCAGGACACAGCCTGGACATGAGGGTCCCTGCTCAACTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCC9980.18066291341576002No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG9940.17993881356239025No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9920.17957676363570535No Hit
CCTGTACAGTGTCCGGATTCGTCTTCGGCAACTTTGCCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGACT9880.17885266378233558No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9880.17885266378233558No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA9840.17812856392896578No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGACACTAAAGTCTGTCCCAGAACCACTCCC9650.17468908962545934No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9460.1712496153219529No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9440.170887565395268No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATG8850.16020709255806376No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8830.15984504263137886No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTG8710.15767274307126952No Hit
CCAGGACACAGCCTGGACATGAGGGTCCCTGCTCAACTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAG8570.1551383935844753No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC8380.15169891928096885No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAACCTC8290.15006969461088684No Hit
CTCCCAGGTCTCTGCTGATACCAATTTAAAGACTTACTAATGTCCTGACTCGCCTGACAAGTGATGGTGACT7910.14319074600387394No Hit
CAGTAATATATGGCCGTGTCCTCGACTCTCAGGCTACTCATTTGCAGATA7880.1426476711138466No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC7820.14156152133379193No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAACCTCATCATATTGTTGAC7810.1413804963704495No Hit
GGACACAGCCTGGACATGAGGGTCCCTGCTCAACTCCTGGGGCTCCTGCT7590.1373979471769157No Hit
CTCTAGTCCCTTCCCTGGAGCCTGGCGGACCCAATTCATGGCAAAGTTGCCGAAGACGAATCCGGACACTGTAC7570.1370358972502308No Hit
GAACAGGGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT7560.13685487228688836No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGC7530.13631179739686103No Hit
AGTCAGGACATTAGTAAGTCTTTAAATTGGTATCAGCAGAGACCTGGGAGAGCCCCTAAACTCCTGATCTACGAA7460.1350446226534639No Hit
GGACATTAGTAAGTCTTTAAATTGGTATCAGCAGAGACCTGGGAGAGCCCCTAAACTCCTGATCTACGAAACATC7440.13468257272677903No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA7390.1337774479100668No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7250.1312430984232726No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC7220.13070002353324522No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7080.128165674046451No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA6920.12526927463297188No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGG6900.12490722470628698No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGGGGAAGACCGATGGGCCCTTGGTGG6860.12418312485291721No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCC6810.123278000036205No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6750.12219185025615033No Hit
GCAGAGACCTGGGAGAGCCCCTAAACTCCTGATCTACGAAACATCCAAGTTGGAAGTAGGGGTCCCATCGAGGAT6730.12182980032946543No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6660.12056262558606831No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6590.1192954508426712No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACC6580.11911442587932877No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT6550.11857135098930142No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6390.11567495157582232No Hit
GCTCAACTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGAGGTGACATCCAGATGACCCAGTCTCCATC6260.11332162705237053No Hit
ATATTACTGTGCGAAAGATATGTTGTATGATCTCCCTGAATATTCGTCGGGTATCTTTGACGACTGGGGCCAGGG6210.1124165022356583No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT6000.10861497800546695No Hit
GGGTTGGTACAGCCTGGGGGGTCCCTGAGAGTCTCCTGTACAGTGTCCGGATTCGTCTTCGGCAACTTTGCCATG5970.10807190311543963No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5880.10644267844535761No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT5850.10589960355533029No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5830.10553755362864539No Hit
CCCCTAAACTCCTGATCTACGAAACATCCAAGTTGGAAGTAGGGGTCCCA5780.10463242881193317No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC5720.1035462790318785No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC5700.1031842291051936No Hit
GTTTAGGGGCTCTCCCAGGTCTCTGCTGATACCAATTTAAAGACTTACTA5690.10300320414185116No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAG5630.10191705436179649No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC5620.10173602939845404No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG5600.10137397947176917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)