FastQCFastQC Report
Wed 8 May 2024
EGAF00004717568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552410
Total Bases40.7 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24610.4455024347857569No Hit
GTAATATATGGCCGTGTCCTCGACTCTCAGGCTACTCATTTGCAGATATA21010.38033344798247676No Hit
CTCCTGTACAGTGTCCGGATTCGTCTTCGGCAACTTTGCCATGAATTGGG17870.3234916094929491No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17030.30828551257218373No Hit
GTTCTGGGACAGACTTTAGTGTCACCATCAGCAGCCTGCAGCCTGAAGAT16290.2948896652848428No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16220.2936224905414457No Hit
GTCCCAACCAGGACACAGCCTGGACATGAGGGTCCCTGCTCAACTCCTGG16100.29145019098133634No Hit
ATATATTACTGTGCGAAAGATATGTTGTATGATCTCCCTGAATATTCGTC15780.2856573921543781No Hit
ATATTACTGTCAACAATATGATGAGGTTCCGCTCACTTTCGGCGGAGGGA14850.2688220705635307No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14390.2604949222497782No Hit
GACCTGGGAGAGCCCCTAAACTCCTGATCTACGAAACATCCAAGTTGGAA14260.2581415977263265No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13700.24800419977914953No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13650.24709907496243733No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA13260.24003910139208198No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13160.23822885175865754No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12970.23478937745515108No Hit
GTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGTAA12550.22718632899476837No Hit
GGACATGAGGGTCCCTGCTCAACTCCTGGGGCTCCTGCTGCTCTGGCTCT12390.22428992958128924No Hit
GACTTTAGTGTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACATA12220.2212125052044677No Hit
GTCCCAGAACCACTCCCACTGATCCTCGATGGGACCCCTACTTCCAACTT12010.21741098097427633No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11950.2163248311942217No Hit
ACATATTACTGTCAACAATATGATGAGGTTCCGCTCACTTTCGGCGGAGG11900.21541970637750946No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11540.20890280769718145No Hit
GCCTGGACATGAGGGTCCCTGCTCAACTCCTGGGGCTCCTGCTGCTCTGG11320.20492025850364767No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11260.20383410872359298No Hit
GGACTAGAGTGGGTCTCAAGGATTAATCGAAGTGGTGGAAGGGCATCCTA11140.20166180916348364No Hit
GGACTGAACAGGGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT11070.20039463442008654No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT10820.19586901033652543No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10760.19478286055647073No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGACACTAA10380.18790391194945785No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10270.18591263735269092No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10200.18464546260929382No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10160.18392136275592405No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10040.1817490631958147No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9960.18030086348907515No Hit
CACTAAAGTCTGTCCCAGAACCACTCCCACTGATCCTCGATGGGACCCCT9860.17849061385565068No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9790.17722343911225358No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGACACT9740.17631831429554135No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9720.17595626436885645No Hit
AACCAGGACACAGCCTGGACATGAGGGTCCCTGCTCAACTCCTGGGGCTC9710.17577523940551404No Hit
CCTGTACAGTGTCCGGATTCGTCTTCGGCAACTTTGCCATGAATTGGGTC9660.17487011458880178No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC9620.174146014735432No Hit
CTCCCAGGTCTCTGCTGATACCAATTTAAAGACTTACTAATGTCCTGACT9350.169258340725186No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9320.16871526583515867No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCC9250.16744809109176156No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9130.16527579153165223No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9080.16437066671494No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8950.1620173421914882No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG8630.15622454336452996No Hit
CCAGGACACAGCCTGGACATGAGGGTCCCTGCTCAACTCCTGGGGCTCCT8610.15586249343784506No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA8510.15405224380442062No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8470.15332814395105085No Hit
AGTCAGGACATTAGTAAGTCTTTAAATTGGTATCAGCAGAGACCTGGGAG8460.1531471189877084No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8460.1531471189877084No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG8360.15133686935428398No Hit
CTCTAGTCCCTTCCCTGGAGCCTGGCGGACCCAATTCATGGCAAAGTTGC8310.15043174453757172No Hit
GGACACAGCCTGGACATGAGGGTCCCTGCTCAACTCCTGGGGCTCCTGCT8260.1495266197208595No Hit
ATATTACTGTGCGAAAGATATGTTGTATGATCTCCCTGAATATTCGTCGG7620.13794102206694303No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7610.1377599971036006No Hit
GGACATTAGTAAGTCTTTAAATTGGTATCAGCAGAGACCTGGGAGAGCCC7590.1373979471769157No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC7550.13667384732354593No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA7460.1350446226534639No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7420.13432052280009413No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC7370.13341539798338192No Hit
CAGTAATATATGGCCGTGTCCTCGACTCTCAGGCTACTCATTTGCAGATA7330.13269129813001213No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7310.13232924820332725No Hit
GAACAGGGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT7170.12979489871653302No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG7110.12870874893647835No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7090.12834669900979345No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGG6930.12545029959631435No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA6900.12490722470628698No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6760.12237287521949278No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA6750.12219185025615033No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6640.12020057565938344No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA6580.11911442587932877No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6530.11820930106261653No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6530.11820930106261653No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT6520.1180282760992741No Hit
GCAGAGACCTGGGAGAGCCCCTAAACTCCTGATCTACGAAACATCCAAGT6490.11748520120924676No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC6410.11603700150250719No Hit
GGGTTGGTACAGCCTGGGGGGTCCCTGAGAGTCTCCTGTACAGTGTCCGG6390.11567495157582232No Hit
GCTCAACTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGAGGTGA6380.11549392661247987No Hit
CCCCTAAACTCCTGATCTACGAAACATCCAAGTTGGAAGTAGGGGTCCCA6270.11350265201571297No Hit
GTCTTTAAATTGGTATCAGCAGAGACCTGGGAGAGCCCCTAAACTCCTGA6190.1120544523089734No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG6190.1120544523089734No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6160.11151137741894607No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC6110.11060625260223385No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT6070.10988215274886406No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT6010.1087960029688094No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6000.10861497800546695No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5960.10789087815209718No Hit
CTCCTGATCTACGAAACATCCAAGTTGGAAGTAGGGGTCCCATCGAGGAT5950.10770985318875473No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT5940.10752882822541228No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT5800.10499447873861804No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5730.10372730399522093No Hit
GTTTAGGGGCTCTCCCAGGTCTCTGCTGATACCAATTTAAAGACTTACTA5730.10372730399522093No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT5730.10372730399522093No Hit
ATATATGGCCGTGTCCTCGACTCTCAGGCTACTCATTTGCAGATATACCG5580.10101192954508427No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)