FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780057
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT38590.49470743804619405No Hit
GTAATATACAGCCGTGTCCTCGACTCTCAGGCTGCTCAGTTGCAGATATAGTGAGTTCTTGGCGTTGTCTCTGG35760.4584280379510728No Hit
GTCTAGAGATGGTGAGGGAGGCAGAATTGGAGGAGCTGTCGATGGAGGCA26190.33574469557993836No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCGGTGGCAACATTGCCAGCAACTCTGTG22500.28844046012022195No Hit
GACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAAAAAGTTCTATCATAAGACTGACAGTAGTAGTC20880.267672746991566No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG20660.2648524402703905No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA19990.25626332434681054No Hit
GTATATTACTGTGCGAGAGGTGGAATGTATGGTGGCTACTTCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCC19540.2504945151444061No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCTAGAGATGGTGAGGGAGGCAGAATTGGAGGAGCTGTCGATGG18580.23818772217927667No Hit
GACTACTACTGTCAGTCTTATGATAGAACTTTTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTACGTC18360.23536741545810114No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18280.2343418493776737No Hit
GCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCA17910.2295986062556967No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTC17210.22062490305195648No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15660.2007545602436745No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15510.19883162384287303No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAC15330.19652410016191124No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC15170.19447296800105635No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14980.19203724856004112No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT14660.1879349842383313No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC14320.1835763283965146No Hit
ATTTTATGCTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACG13780.1766537573536293No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC13620.17460262519277436No Hit
GCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCGGTGGCAACATTGCC13600.1743462336726675No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13570.17396164639250722No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT13470.1726796887919729No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13440.1722951015118126No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13420.17203870999170573No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13200.16921840327053023No Hit
ATTTAGTCCCTCCGCCGAACACCCAAAAAGTTCTATCATAAGACTGACAG13160.16870562023031652No Hit
CTACTACTGTCAGTCTTATGATAGAACTTTTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTACGTCAGCC12500.16024470006678998No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCTAGAGATGGTGAGGGA12460.15973191702657627No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC12440.1594755255064694No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12430.15934732974641597No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCTAGAGATGGTGAGGGAGGCAGAATTGG12160.1558860442249733No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTG11740.15050182230272915No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACAC11670.14960445198235514No Hit
CTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCGGTGGCAACATTGCCAGCAACT11470.1470405367812865No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCC11450.1467841452611796No Hit
GTGCAGTGGTCCCAACAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTA11430.14652775374107277No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC11420.14639955798101933No Hit
GTCTTATGATAGAACTTTTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTACGTCAGCCCAAGGCTGCCCC11170.14319466397968356No Hit
GTCCCAACAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGACGATA11000.1410153360587752No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10830.13883600813786684No Hit
GGTCCCAACAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGACGATAATGAAAGACCCTCTGGGGTCCCTG10780.1381950293375997No Hit
GTTCTCTGCCTCCATCGACAGCTCCTCCAATTCTGCCTCCCTCACCATCTCTAGACTGAAGACTGAGGACGAGG10350.1326826116553021No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATG10230.13114426253466094No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10210.13088787101455404No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10120.1297341091740732No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC10100.12947771765396632No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9920.12717019397300452No Hit
GTCTTCAGTCTAGAGATGGTGAGGGAGGCAGAATTGGAGGAGCTGTCGATGGAGGCAGAGAACCGATCAGGGACC9800.12563184485236337No Hit
AATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG9770.12524725757220306No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA9700.12434988725182902No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9590.12293973389124128No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCGCACT9590.12293973389124128No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG9550.12242695085102756No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC9550.12242695085102756No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCT9550.12242695085102756No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9540.12229875509097413No Hit
CTCCAGACCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGCTATAAGAACTGAAGCTGAATCCAGAGGCTGCACA9410.1206322102102795No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA9370.12011942717006577No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAAAAAGTT9360.11999123141001236No Hit
GAATTGGAGGAGCTGTCGATGGAGGCAGAGAACCGATCAGGGACCCCAGAGGGTCTTTCATTATCGTCATAGATC9300.11922205684969177No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC9260.11870927380947802No Hit
ACTCTGTGCAGTGGTCCCAACAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGACGATAATGAAAGACCCT9210.11806829500921087No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACT8960.11486340100787507No Hit
CTCTTGTGCAGCCTCTGGATTCAGCTTCAGTTCTTATAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGT8800.11281226884702014No Hit
GGACTAAATTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGG8660.1110175282062721No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC8510.10909459180547063No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATG8480.10871000452531032No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8470.10858180876525689No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8450.10832541724515005No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8360.10717165540466915No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTC8320.10665887236445543No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG8280.10614608932424169No Hit
ACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAAAAAGTTCTATCATAAGACTGACAGTAGTAGTCAGC8050.10319758684301276No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTT8020.10281299956285245No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8020.10281299956285245No Hit
GCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCGCACTCTGTGTCG8010.10268480380279901No Hit
GTTGGGACCACTGCACAGAGTTGCTGGCAATGTTGCCACCGCTGCGGGTG8010.10268480380279901No Hit
CCCCAGAGGGTCTTTCATTATCGTCATAGATCACAGTGGTGGGGGCACTGCCCGGGCGCTGTTGGGACCACTGC7950.10191562924247843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)