FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780057
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT35410.4539411863492027No Hit
GTAATATACAGCCGTGTCCTCGACTCTCAGGCTGCTCAGTTGCAGATATAGTGAGTTCTTGGCGTTGTCTCTGG35140.45047990082776No Hit
GTCTAGAGATGGTGAGGGAGGCAGAATTGGAGGAGCTGTCGATGGAGGCA26380.3381804150209536No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCGGTGGCAACATTGCCAGCAACTCTGTG20860.26741635547145914No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA20220.2592118268280395No Hit
GACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAAAAAGTTCTATCATAAGACTGACAGTAGTAGTC20060.25716069466718455No Hit
GTATATTACTGTGCGAGAGGTGGAATGTATGGTGGCTACTTCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCC18800.24100802890045214No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCTAGAGATGGTGAGGGAGGCAGAATTGGAGGAGCTGTCGATGG18720.23998246282002472No Hit
GACTACTACTGTCAGTCTTATGATAGAACTTTTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTACGTC18310.23472643665783396No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG18250.23395726209751339No Hit
GCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCA17700.2269064952945746No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTC16620.21306135320880398No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16490.21139480832810933No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15950.204472237285224No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAC15810.202677496644476No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC15530.1990880153629799No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15210.19498575104127006No Hit
ATTTTATGCTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACG14340.18383271991662148No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT14010.17960225983485822No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACAC13660.1751154082329881No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC13530.17344886335229348No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13500.1730642760721332No Hit
ATTTAGTCCCTCCGCCGAACACCCAAAAAGTTCTATCATAAGACTGACAG13100.1679364456699959No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12870.16498794318876697No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC12730.16319320254801895No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC12680.16255222374775175No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12600.16152665766732432No Hit
GGTCCCAACAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGACGATAATGAAAGACCCTCTGGGGTCCCTG12510.16037289582684341No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCTAGAGATGGTGAGGGAGGCAGAATTGG12370.15857815518609536No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC11890.15242475870353064No Hit
GCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCGGTGGCAACATTGCC11890.15242475870353064No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCTAGAGATGGTGAGGGA11850.1519119756633169No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11780.1510146053429429No Hit
GTTCTCTGCCTCCATCGACAGCTCCTCCAATTCTGCCTCCCTCACCATCTCTAGACTGAAGACTGAGGACGAGG11780.1510146053429429No Hit
CTACTACTGTCAGTCTTATGATAGAACTTTTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTACGTCAGCC11670.14960445198235514No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11640.14921986470219484No Hit
GTCTTATGATAGAACTTTTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTACGTCAGCCCAAGGCTGCCCC11590.14857888590192767No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11050.14165631485904237No Hit
GTGCAGTGGTCCCAACAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTA10960.14050255301856146No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10680.13691307173706535No Hit
GTCCCAACAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGACGATA10420.13357998197567614No Hit
CTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCGGTGGCAACATTGCCAGCAACT10410.1334517862156227No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG10380.1330671989354624No Hit
GTCTTCAGTCTAGAGATGGTGAGGGAGGCAGAATTGGAGGAGCTGTCGATGGAGGCAGAGAACCGATCAGGGACC10220.13101606677460748No Hit
GAGTTGCTGGCAATGTTGCCACCGCTGCGGGTGCAGGAGATGGTTACCGT10110.12960591341401975No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9720.12460627877193589No Hit
GTTGGGACCACTGCACAGAGTTGCTGGCAATGTTGCCACCGCTGCGGGTG9570.12268334237113442No Hit
GAATTGGAGGAGCTGTCGATGGAGGCAGAGAACCGATCAGGGACCCCAGAGGGTCTTTCATTATCGTCATAGATC9520.12204236357086726No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9410.1206322102102795No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9330.11960664412985204No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA9180.11768370772905057No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC9080.11640175012851625No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATG9040.11588896708830253No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC9030.11576077132824909No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8980.11511979252798192No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAAAAAGTT8970.1149915967679285No Hit
GGACTAAATTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGG8950.11473520524782163No Hit
CTCCGACACAGAGTGCGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTG8940.1146070094877682No Hit
CTCCAGACCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGCTATAAGAACTGAAGCTGAATCCAGAGGCTGCACA8940.1146070094877682No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8900.11409422644755449No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8660.1110175282062721No Hit
AATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG8400.10768443844488286No Hit
CTGTTGGGACCACTGCACAGAGTTGCTGGCAATGTTGCCACCGCTGCGGGTGCAGGAGATGGTTACCGTCTTCCC8340.10691526388456228No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG8240.10563330628402796No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTC8190.1049923274837608No Hit
ACTCTGTGCAGTGGTCCCAACAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGACGATAATGAAAGACCCT8190.1049923274837608No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACC8160.1046077402036005No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8160.1046077402036005No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC8120.10409495716338678No Hit
GCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCGCACTCTGTGTCG8080.10358217412317304No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA8070.1034539783631196No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8000.1025566080427456No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7870.10089006316205099No Hit
CTCTTGTGCAGCCTCTGGATTCAGCTTCAGTTCTTATAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGT7860.10076186740199755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)