FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930004
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCTCCAGTCTGGTAGGTCACGTACGTGGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGA43040.46279370841415735No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGCCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC31110.33451469025939673No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29670.31903088588866285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT26390.2837622203775468No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAGC26180.2815041655734814No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGCCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC25710.27645042386914465No Hit
GTGTATTACTGTCTCCAGTCTGGTAGGTCACGTACGTGGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGA23900.25698814198648606No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19430.2089238325856663No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAGCGACTTAGGCTGGTACC19030.20462277581601798No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18990.20419267013905315No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG16620.17870890877888695No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA16170.17387021991303264No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT16160.17376269349379142No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCGGTGGCCCTGCT15970.17171969152820848No Hit
GTAATACACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTAGGCTGTGGTCGCGGATTCGTCCGCAAT15570.16741863475856017No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15400.16559068563145965No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC14980.16107457602332892No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG14870.15989178541167565No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGGCTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCTCCAGT14750.15860146838078115No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14320.15397783235340926No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA14280.1535477266764444No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14200.15268751532251473No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14180.15247246248403232No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG14040.15096709261465543No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTGGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGC13890.1493541963260373No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13660.14688108868348954No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGC13660.14688108868348954No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAGC13460.14473056029866538No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13390.14397787536397694No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12620.13569834108240394No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAA12410.1334402862783386No Hit
GTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCCCTTGGCCGAACGTCCACGTACGTGACCTACCAGACTGG12050.1295693351856551No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12030.1293542823471727No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCG11940.12838654457400184No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGC11890.1278489124777958No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11700.12580591051221285No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA11660.12537580483524802No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC11580.12451559348131837No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCCCTTGGCCGAACGTCCACGTACGTG11430.12290269719270025No Hit
GAAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCAGGGTGCATCCAGCAGGGCCACCGGCATCCCAGACAGGTT11390.12247259151573543No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11100.11935432535774039No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11050.11881669326153435No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10940.11763390264988108No Hit
GACTTCACTCTCACCATCAGCAGGCTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCTCCAGTCTGGTAGGTC10910.11731132339215745No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10610.11408553081492122No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10450.1123651081070619No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC10380.11161242317237346No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG10360.11139737033389105No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC10340.11118231749540862No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10020.10774147207968998No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAGCACCGGAGAAATTGTGTTGACGCAGTCTC10000.10752641924120757No Hit
GTCAGAGTGTCAGCAGCAGCGACTTAGGCTGGTACCAGAAGAAACCTGGC9900.10645115504879549No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9790.10526836443714221No Hit
CCCCTGGACAAGGGCCTGAGTGGATGGGAGAGATCATCCCTATCTTTAGTACAACAAACTACGCACAGAAGTTTC9690.10419310024473014No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA9540.10258020395611202No Hit
CAGTAATACACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTAGGCTGTGGTCGCGGATTCGTCCGCA9390.1009673076674939No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9330.10032214915204665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)