FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930004
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCTCCAGTCTGGTAGGTCACGTACGTGGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGA40990.44075079246970983No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29130.31322445924963765No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGCCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC28340.30472987212958225No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGCCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC25630.275590212515215No Hit
GTGTATTACTGTCTCCAGTCTGGTAGGTCACGTACGTGGACGTTCGGCCAAGGGACCAAGGTAGAAATCAAACGA24250.2607515666599283No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT24150.2596763024675163No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAGC22970.24698818499705377No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18210.19580560943823896No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18090.1945152924073445No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAGCGACTTAGGCTGGTACC17300.1860207052872891No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA16860.18128954284067597No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG15600.1677412140162838No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT15360.16516057995449482No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15100.16236489305422344No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15050.1618272609580174No Hit
GTAATACACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTAGGCTGTGGTCGCGGATTCGTCCGCAAT15030.16161220811953497No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGGCTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCTCCAGT14880.15999931183091684No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCGGTGGCCCTGCT14710.15817136270381635No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTGGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGC14190.15257998890327354No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG13570.14591335091031868No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13530.14548324523335382No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA13460.14473056029866538No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12820.1378488694672281No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12750.13709618453253963No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAGC12460.13397791837454462No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGC12330.13258007492440893No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12330.13258007492440893No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC12270.13193491640896168No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAA12020.1292467559279315No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCG11950.12849407099324303No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA11930.12827901815476062No Hit
GTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCCCTTGGCCGAACGTCCACGTACGTGACCTACCAGACTGG11610.12483817273904198No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11430.12290269719270025No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC11320.12171990658104696No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC11190.12032206313091127No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA11130.11967690461546401No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11120.1195693781962228No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC11090.1192467989384992No Hit
GACTTCACTCTCACCATCAGCAGGCTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCTCCAGTCTGGTAGGTC10970.11795648190760469No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG10940.11763390264988108No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10890.11709627055367504No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCCCTTGGCCGAACGTCCACGTACGTG10780.11591347994202175No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG10540.11333284588023278No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10510.11301026662250915No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10420.11204252884933828No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10410.11193500243009707No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGC10240.11010705330299654No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10230.10999952688375535No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9920.1066662078872779No Hit
GAAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCAGGGTGCATCCAGCAGGGCCACCGGCATCCCAGACAGGTT9790.10526836443714221No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9640.1036554681485241No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9610.10333288889080047No Hit
GTCCAGGGGCCTGTCGCACCCAGCTGACACCATAGCTGCTGAAGGTGCCTCCAGAAGCCTTGCAGGAGACCTTC9440.10150493976369994No Hit
CTGCTACTCTGGCTCCCAGAAAGCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCC9420.10128988692521751No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA9400.10107483408673512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)