FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038806
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATACTGACTCAGCCACCCTCAGTGTCGGTGACCCCAGGAGAGACGGCCAAGATTTCCTGTGCGGGAGACA79610.7663606101620514No Hit
GGATAATAGTGCTGACAGTCGGGTCTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCCAAGGCTGCCCC32320.3111264278411946No Hit
CTATTATCCCACACCTGACAGAAATAGTCGGCCTCATCCCCGGCTCCGACCCCGCTGATGGTCAGGGTGGCCGTG30460.29322125594191795No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27450.2642456820619057No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCGCACCCTCCTCTGAGGAGC27150.26135775111040943No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA25010.24075717698973628No Hit
CCCCAGGAGAGACGGCCAAGATTTCCTGTGCGGGAGACAATATTGGGAGTAAAGCTGTACACTGGTACCAACAG23510.2263175222322551No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20760.1998448218435396No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT20560.1979195345425421No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19780.1904109140686519No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC19150.1843462590705098No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC18780.18078447756366445No Hit
CCTCAGAGGAGGGTGCGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGC18250.1756824662160211No Hit
GTTCGCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG18160.1748160869305722No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC17240.16595976534598375No Hit
GTGTACAGCTTTACTCCCAATATTGTCTCCCGCACAGGAAATCTTGGCCGTCTCTCCTGGGGTCACCGACACTG17200.16557470788578424No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17000.16364942058478676No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16780.16153160455368953No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC16620.15999137471289152No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16240.1563333288409963No Hit
CGGCCACCCTGACCATCAGCGGGGTCGGAGCCGGGGATGAGGCCGACTATTTCTGTCAGGTGTGGGATAATAGTG15700.15113505312830305No Hit
GCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAAGACCCGACTGTCAGCACTATTATCCCACAC15590.15007614511275447No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATACTGACT15520.14940229455740534No Hit
CCCTCAGTGTCGGTGACCCCAGGAGAGACGGCCAAGATTTCCTGTGCGGGAGACAATATTGGGAGTAAAGCTGT15400.14824712217680683No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15380.14805459344670707No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGCGAACAGAGTGACCGAGGGGGCAGCCTTGGG15070.14507039813016095No Hit
GTAGGGGGGCCTGGCCTGGCCTCTGTTGGTACCAGTGTACAGCTTTACTC14830.14276005336896397No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCTCTGGTGGTGCCTCAGCCATGGCCTGGACCGTT14590.14044970860776698No Hit
GCTGTACACTGGTACCAACAGAGGCCAGGCCAGGCCCCCCTACTGGTCATCTTTTATGATACCGACCGGCCCTC14580.14035344424271712No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14380.13842815694171964No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14290.13756177765627076No Hit
GCTCTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTG13940.13419252487952515No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGATGGTTTCTTGCA13910.1339037317843755No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA13760.1324597663086274No Hit
GTATATAGGAGGTCACAGAGCCTGTGCAGTGAGAGAGGAGGCCGAGGAGGAGAACGGTCCAGGCCATGGCTGAGG13230.12735775496098406No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG13170.12678016877068482No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATG13110.12620258258038555No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12900.1241810309143382No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT12830.12350718035898907No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCGCACCCTC12780.1230258585337397No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12540.1207155137725427No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATACTGACTCAGCC12520.12052298504244296No Hit
GGCTCTGTGACCTCCTATATACTGACTCAGCCACCCTCAGTGTCGGTGACCCCAGGAGAGACGGCCAAGATTTC12380.1191752839317447No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATACTGACTCAGCCACCCTCAGTGTCGGTGACCCCAGGAG12360.11898275520164496No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC12310.1185014333763956No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12260.11802011155114622No Hit
CTGTTGGTACCAGTGTACAGCTTTACTCCCAATATTGTCTCCCGCACAGGAAATCTTGGCCGTCTCTCCTGGGGT12220.11763505409094673No Hit
GAAGTAGACGGCTGTGTCTTCAGATTTCAGACCGGCCAGTTGCATGTAGGCCGTGCTCGCGGATGGGTCCCTAGT12070.1161910886151986No Hit
CTCCATTGGGTGCGCCAGGCCCCCGGACAAAGCCTTGAGTGGATGGGATGGATTAACACTGCCAATGGCAACGC11890.11445833004430087No Hit
GCCCTCAGGGATCCCTGACCGATTCGTCGGCTCCAACAAGGGGGGCACGGCCACCCTGACCATCAGCGGGGTCGG11500.11070401980735574No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCGCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11470.11041522671220612No Hit
ATATTGGGAGTAAAGCTGTACACTGGTACCAACAGAGGCCAGGCCAGGCCCCCCTACTGGTCATCTTTTATGAT11410.10983764052190689No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11270.10848993941120863No Hit
GCACAGGCTCTGTGACCTCCTATATACTGACTCAGCCACCCTCAGTGTCGGTGACCCCAGGAGAGACGGCCA11260.10839367504615877No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11190.10771982449080963No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCTCTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG11150.10733476703061015No Hit
GGCCAGGCCAGGCCCCCCTACTGGTCATCTTTTATGATACCGACCGGCCC11140.10723850266556027No Hit
GTTGGTACCAGTGTACAGCTTTACTCCCAATATTGTCTCCCGCACAGGAAATCTTGGCCGTCTCTCCTGGGGTC10860.10454310044416379No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10700.10300287060336578No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA10650.10252154877811641No Hit
CTCCTGGGGTCACCGACACTGAGGGTGGCTGAGTCAGTATATAGGAGGTCACAGAGCCTGTGCAGTGAGAGAGG10640.10242528441306653No Hit
GTATCATAAAAGATGACCAGTAGGGGGGCCTGGCCTGGCCTCTGTTGGTA10640.10242528441306653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)