FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038806
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATACTGACTCAGCCACCCTCAGTGTCGGTGACCCCAGGAGAGACGGCCAAGATTTCCTGTGCGGGAGACA63120.6076206721948083No Hit
CTATTATCCCACACCTGACAGAAATAGTCGGCCTCATCCCCGGCTCCGACCCCGCTGATGGTCAGGGTGGCCGTG30440.29302872721181816No Hit
GGATAATAGTGCTGACAGTCGGGTCTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCCAAGGCTGCCCC29230.28138073904078337No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCGCACCCTCCTCTGAGGAGC26550.255581889207417No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA25810.24845832619372626No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25500.24547413087718015No Hit
GGGTGGCCGTGCCCCCCTTGTTGGAGCCGACGAATCGGTCAGGGATCCCTGAGGGCCGGTCGGTATCATAAA25220.24277872865578368No Hit
GCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAAGACCCGACTGTCAGCACTATTATCCCACAC22060.2123591893000233No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT19270.18550143145110828No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC18970.18261350049961206No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18670.1797255695481158No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC18640.17943677645296618No Hit
CCTCAGAGGAGGGTGCGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGC18520.1782816040723677No Hit
CCCCAGGAGAGACGGCCAAGATTTCCTGTGCGGGAGACAATATTGGGAGTAAAGCTGTACACTGGTACCAACAG18430.17741522478691882No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18360.1767413742315697No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18290.17606752367622058No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC17830.17163936288392634No Hit
GTTCGCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG16660.16037643217309103No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16460.15845114487209355No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC15900.15306034042930058No Hit
GTGTACAGCTTTACTCCCAATATTGTCTCCCGCACAGGAAATCTTGGCCGTCTCTCCTGGGGTCACCGACACTG15850.1525790186040512No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15520.14940229455740534No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14430.13890947876696902No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCTCTGGTGGTGCCTCAGCCATGGCCTGGACCGTT14400.1386206856718194No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGCGAACAGAGTGACCGAGGGGGCAGCCTTGGG14030.13505890416497401No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13960.13438505360962488No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATACTGACT13930.13409626051447526No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13570.13063074337267977No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13530.1302456859124803No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13480.1297643640872309No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT13280.1278390767862334No Hit
GTAGGGGGGCCTGGCCTGGCCTCTGTTGGTACCAGTGTACAGCTTTACTC13220.1272614905959342No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG12890.12408476654928832No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGATGGTTTCTTGCA12840.12360344472403895No Hit
GCTCTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTG12710.12235200797839058No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATG12700.1222557436133407No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA12530.12061924940749283No Hit
CCTATATACTGACTCAGCCACCCTCAGTGTCGGTGACCCCAGGAGAGACGGCCAAGATTTCCTGTGCGGGAGAC12220.11763505409094673No Hit
CGGCCACCCTGACCATCAGCGGGGTCGGAGCCGGGGATGAGGCCGACTATTTCTGTCAGGTGTGGGATAATAGTG12170.11715373226569735No Hit
GTATATAGGAGGTCACAGAGCCTGTGCAGTGAGAGAGGAGGCCGAGGAGGAGAACGGTCCAGGCCATGGCTGAGG12110.1165761460753981No Hit
GAAGTAGACGGCTGTGTCTTCAGATTTCAGACCGGCCAGTTGCATGTAGGCCGTGCTCGCGGATGGGTCCCTAGT12080.11628735298024846No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAAGACCCGACTGTC12050.11599855988509887No Hit
CTGTTGGTACCAGTGTACAGCTTTACTCCCAATATTGTCTCCCGCACAGGAAATCTTGGCCGTCTCTCCTGGGGT12030.11580603115499911No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC12020.11570976678994924No Hit
GCTGTACACTGGTACCAACAGAGGCCAGGCCAGGCCCCCCTACTGGTCATCTTTTATGATACCGACCGGCCCTC11970.11522844496469986No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11510.11080028417240562No Hit
CCCTCAGTGTCGGTGACCCCAGGAGAGACGGCCAAGATTTCCTGTGCGGGAGACAATATTGGGAGTAAAGCTGT11300.10877873250635826No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11240.10820114631605901No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCGCACCCTC11070.10656465211021116No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11020.10608333028496178No Hit
GACTCAGGACGGTCAACTTGGTCCCGCCGCCGAAGACCCGACTGTCAGCACTATTATCCCACACCTGACAGAAAT10650.10252154877811641No Hit
GTTGGTACCAGTGTACAGCTTTACTCCCAATATTGTCTCCCGCACAGGAAATCTTGGCCGTCTCTCCTGGGGTC10640.10242528441306653No Hit
GGCTCTGTGACCTCCTATATACTGACTCAGCCACCCTCAGTGTCGGTGACCCCAGGAGAGACGGCCAAGATTTC10620.10223275568296679No Hit
CTCCATTGGGTGCGCCAGGCCCCCGGACAAAGCCTTGAGTGGATGGGATGGATTAACACTGCCAATGGCAACGC10600.10204022695286703No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATACTGACTCAGCC10500.1010775833023683No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10430.10040373274701918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)