FastQCFastQC Report
Wed 8 May 2024
EGAF00004717577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332995
Total Bases24.5 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19330.5804891965344825No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18140.5447529242180813No Hit
CTCCTAAACTACTCATTTACTGGGCTTCTACCCGGGTCTCCGGGGTCCCT15480.4648718449225964No Hit
GTTTAGGAGGCTGCCCTGGTTTCTGCTGGAACCAAGCTACGTAGTTCTGA14740.4426492890283638No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12870.38649228967401916No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12310.3696752203486539No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12230.3672727818736017No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11650.3498551029294734No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11600.3483535788825658No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11520.3459511404075136No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCA11250.33784291055421256No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10840.3255304133695701No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10370.31141608732863857No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10040.3015060286190483No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9490.28498926410306463No Hit
GTCCCTGACCGATTCAGTGGCAGCGCGTCTGGGACAGATTTCAGTCTCAC9410.2825868256280124No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9350.2807849967717233No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9320.27988408234357876No Hit
GTTTATTACTGTCAGCAATATTTTACTACTCCGTACACTTTTGGCCAGGG9140.27447859577471134No Hit
TTACTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGA9110.2735776813465668No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC8880.26667067073079176No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC8680.2606645745431613No Hit
ATTCAGTGGCAGCGCGTCTGGGACAGATTTCAGTCTCACCATCAGCAGCC8620.2588627456868722No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA8420.2528566494992417No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC8390.25195573507109714No Hit
GAACTACGTAGCTTGGTTCCAGCAGAAACCAGGGCAGCCTCCTAAACTAC8250.24775146773975587No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCACCTGCAGGTCCAGC8000.2402438475052178No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCC7920.2378414090301656No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGTG7630.22913256955810146No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGACTGA7590.22793135032057538No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCACCTGCAGGTCCA7550.22673013108304932No Hit
GTCTCCGGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCACCT7360.22102433970480037No Hit
GGCCAAAAGTGTACGGAGTAGTAAAATATTGCTGACAGTAATAAACTGCC7320.21982312046727429No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7280.21862190122974823No Hit
GTAATAAACGGCCGTGTCGTCAGATTTCAGTCTGTTCAATTCCATGTAGG7020.21081397618582862No Hit
GTCAGGGACCCCGGAGACCCGGGTAGAAGCCCAGTAAATGAGTAGTTTAG6940.20841153771077645No Hit
ACGTAGTTCTGACTGTTGGACCTGTAAAAAACACTCTGGCTGGACCTGCA6760.20300605114190903No Hit
GAGTAGTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGC6640.19940239342933078No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6630.19910208861994924No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT6620.19880178381056773No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6590.19790086938242316No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA6440.19339629724170032No Hit
AGTCAGAACTACGTAGCTTGGTTCCAGCAGAAACCAGGGCAGCCTCCTAA6350.19069355395726661No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC6340.1903932491478851No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG6270.18829111548221444No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG5960.17898166639138724No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5740.17237496058499377No Hit
GTCTCTGGGCGAGAGGGCCACCATCACCTGCAGGTCCAGCCAGAGTGTTT5720.17177435096623073No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5710.1714740461568492No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC5660.1699725221099416No Hit
GTTTTTTACAGGTCCAACAGTCAGAACTACGTAGCTTGGTTCCAGCAGAA5650.16967221730056006No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC5600.16817069325365247No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA5550.16666916920674485No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5540.16636886439736334No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5440.1633658163035481No Hit
GATTTCAGTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA5410.16246490187540352No Hit
GGTCCAGCCAGAGTGTTTTTTACAGGTCCAACAGTCAGAACTACGTAGCT5410.16246490187540352No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTACTACTCCGT5380.16156398744725897No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC5320.15976215859096984No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT5240.15735972011591765No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT5230.15705941530653614No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5230.15705941530653614No Hit
GTTCTGACTGTTGGACCTGTAAAAAACACTCTGGCTGGACCTGCAGGTGA5200.1561585008783916No Hit
GTAAAAAACACTCTGGCTGGACCTGCAGGTGATGGTGGCCCTCTCGCCCA5000.15015240469076113No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5000.15015240469076113No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC4930.14805027102509047No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA4880.14654874697818285No Hit
ATATTTTACTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGCTCA4860.14594813735941983No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG4860.14594813735941983No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG4820.14474691812189372No Hit
GGTTTCTGCTGGAACCAAGCTACGTAGTTCTGACTGTTGGACCTGTAAAA4810.14444661331251218No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCGGACTCC4790.14384600369374917No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC4780.14354569888436763No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCGGACTCCCTGGCTGTGTCTC4700.14114326040931546No Hit
ACGTAGCTTGGTTCCAGCAGAAACCAGGGCAGCCTCCTAAACTACTCATT4700.14114326040931546No Hit
GGTCCAACAGTCAGAACTACGTAGCTTGGTTCCAGCAGAAACCAGGGCAG4700.14114326040931546No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT4680.1405426507905524No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA4660.13994204117178938No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG4650.13964173636240784No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC4630.1390411267436448No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT4630.1390411267436448No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT4590.13783990750611871No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT4560.13693899307797414No Hit
GTTTATTACTGTGCGAGAGGAGGGACAAATTTTCGGGGGAAGACGAACGA4550.1366386882685926No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC4550.1366386882685926No Hit
CTGTTGGACCTGTAAAAAACACTCTGGCTGGACCTGCAGGTGATGGTGGC4530.13603807864982959No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGACT4480.13453655460292196No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGACTGAAATCTGTCCCAGACGC4460.13393594498415892No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC4410.1324344209372513No Hit
TTATTACTGTCAGCAATATTTTACTACTCCGTACACTTTTGGCCAGGGGA4400.1321341161278698No Hit
GGTCTCCGGGGTCCCTGACCGATTCAGTGGCAGCGCGTCTGGGACAGATT4390.1318338113184883No Hit
ATTTTACTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGCTCAAA4370.13123320169972522No Hit
GCCACTGAATCGGTCAGGGACCCCGGAGACCCGGGTAGAAGCCCAGTAAA4360.1309328968903437No Hit
GTAGTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAG4340.13033228727158064No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC4300.12913106803405455No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC4290.12883076322467304No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC4290.12883076322467304No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT4280.1285304584152915No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG4170.1252271055120948No Hit
GAATCGGTCAGGGACCCCGGAGACCCGGGTAGAAGCCCAGTAAATGAGTA4150.12462649589333173No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA4040.121323142990135No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA4030.12102283818075346No Hit
GACCGATTCAGTGGCAGCGCGTCTGGGACAGATTTCAGTCTCACCATCAG4000.1201219237526089No Hit
GTGTACGGAGTAGTAAAATATTGCTGACAGTAATAAACTGCCACATCTTC4000.1201219237526089No Hit
GCAGAAACCAGGGCAGCCTCCTAAACTACTCATTTACTGGGCTTCTACCC4000.1201219237526089No Hit
CTATTTGCACTGGGTGCGACAAGTCCCTGGACAAGGGCCTGAGTGGGTGG3950.11862039970570129No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG3950.11862039970570129No Hit
AACCAGGGCAGCCTCCTAAACTACTCATTTACTGGGCTTCTACCCGGGTC3910.1174191804681752No Hit
ACCATCACCTGCAGGTCCAGCCAGAGTGTTTTTTACAGGTCCAACAGTCA3900.11711887565879367No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA3870.1162179612306491No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA3810.11441613237435998No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTG3800.11411582756497844No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA3800.11411582756497844No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA3750.11261430351807084No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT3730.1120136938993078No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG3720.11171338908992627No Hit
AAAGTGTACGGAGTAGTAAAATATTGCTGACAGTAATAAACTGCCACATC3720.11171338908992627No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCT3690.1108124746617817No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGT3670.11021186504301868No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT3620.10871034099611104No Hit
GTCTGGGACAGATTTCAGTCTCACCATCAGCAGCCTGCAGGCTGAAGATG3590.10780942656796648No Hit
GTAAATGAGTAGTTTAGGAGGCTGCCCTGGTTTCTGCTGGAACCAAGCTA3590.10780942656796648No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG3580.10750912175858496No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC3560.10690851213982192No Hit
GTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT3540.10630790252105889No Hit
CAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATG3540.10630790252105889No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT3530.10600759771167735No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG3500.10510668328353279No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC3490.10480637847415125No Hit
GGCTGGACCTGCAGGTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGG3480.10450607366476974No Hit
GGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC3440.10330485442724366No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT3440.10330485442724366No Hit
GCCCTGGTTTCTGCTGGAACCAAGCTACGTAGTTCTGACTGTTGGACCTG3420.10270424480848062No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT3420.10270424480848062No Hit
ACTCATTTACTGGGCTTCTACCCGGGTCTCCGGGGTCCCTGACCGATTCA3390.10180333038033605No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC3370.101202720761573No Hit
GCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGG3340.10030180633342843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)