FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332995
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16610.4988062883827084No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT13950.4189252090872235No Hit
CTCCTAAACTACTCATTTACTGGGCTTCTACCCGGGTCTCCGGGGTCCCT12280.36877430592050936No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11890.35706241835462993No Hit
GTTTAGGAGGCTGCCCTGGTTTCTGCTGGAACCAAGCTACGTAGTTCTGACTGTTGGACCTGTAAAAAACACT11220.33694199612606796No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10430.3132179161849277No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA9880.29670115166894395No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG9860.29610054205018094No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT9810.2945990180032733No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9640.28949383624378744No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGACTGAAATCTGTCCCAGACGCGCTGCC9350.2807849967717233No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG8700.26126518416192435No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC8580.2576615264493461No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG7850.23573927536449496No Hit
GGCCAAAAGTGTACGGAGTAGTAAAATATTGCTGACAGTAATAAACTGCC7670.23033378879562758No Hit
ATTCAGTGGCAGCGCGTCTGGGACAGATTTCAGTCTCACCATCAGCAGCC7540.22642982627366776No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7500.22522860703614167No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC7440.22342677817985254No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC7440.22342677817985254No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG7360.22102433970480037No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC7240.2174206819922221No Hit
GTTTATTACTGTCAGCAATATTTTACTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGA7240.2174206819922221No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGTAGTAAAATATTGCTGAC7200.21621946275469603No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7150.2147179387077884No Hit
GTCCCTGACCGATTCAGTGGCAGCGCGTCTGGGACAGATTTCAGTCTCAC6900.20721031847325036No Hit
TTACTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGA6870.2063094040451058No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC6790.2039069655700536No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA6690.20090391747623837No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6690.20090391747623837No Hit
GAGTAGTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGACTGAAAT6660.2000030030480938No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGTAGT6570.19730025976366009No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCACCTGCAGGTCCAGCCAGAGTGTTTTTTACAGGTCCAAC6320.18979263952912206No Hit
GTAATAAACGGCCGTGTCGTCAGATTTCAGTCTGTTCAATTCCATGTAGGCTGTGGTGATGGACGTGTCCCTGGT6260.18799081067283294No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6230.18708989624468836No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6120.1837865433414916No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6060.18198471448520248No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC5930.1780807519632427No Hit
GTCTCCGGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCACCTGCAGGTCCAGCCAGAGTGTTTTTT5880.17657922791633507No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT5730.17207465577561226No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGACTGAAATCTGTCCCAGACGCGCTGCCACTGAATCGGTCAGGGACC5710.1714740461568492No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGACTGAAATCTGTCCCAGACGCGCTGCC5670.17027282691932313No Hit
GAACTACGTAGCTTGGTTCCAGCAGAAACCAGGGCAGCCTCCTAAACTACTCATTTACTGGGCTTCTACCCGGGT5660.1699725221099416No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5480.16456703554107419No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTACTACTCCGTACACTTTTGGCCAGGGGACCA5210.1564588056877731No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5210.1564588056877731No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5180.15555789125962852No Hit
GTCCCAGACGCGCTGCCACTGAATCGGTCAGGGACCCCGGAGACCCGGGTAGAAGCCCAGTAAATGAGTAGTTT5050.15165392873766873No Hit
GTCAGGGACCCCGGAGACCCGGGTAGAAGCCCAGTAAATGAGTAGTTTAGGAGGCTGCCCTGGTTTCTGCTGGA4960.14895118545323502No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC4940.14835057583447198No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4890.14684905178756438No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT4860.14594813735941983No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT4760.1429450892656046No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC4750.14264478445622306No Hit
GTCTCTGGGCGAGAGGGCCACCATCACCTGCAGGTCCAGCCAGAGTGTTTTTTACAGGTCCAACAGTCAGAACT4740.14234447964684155No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT4650.13964173636240784No Hit
GTGTACGGAGTAGTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTG4650.13964173636240784No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCACCTGCAGGTCCAGCCAGAGTGTTTTTTACAGGTCCA4620.13874082193426326No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA4600.13814021231550025No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC4560.13693899307797414No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG4560.13693899307797414No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4530.13603807864982959No Hit
ATTTTACTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGCTCAAA4450.13363564017477741No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC4440.13333533536539588No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT4400.1321341161278698No Hit
GATTTCAGTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTACTACT4320.12973167765281762No Hit
ATATTTTACTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCAT4260.1279298487965285No Hit
GTAGTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGACTGAAAT4260.1279298487965285No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC4230.12702893436838392No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4230.12702893436838392No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG4180.1255274103214763No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA4180.1255274103214763No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG4140.12432619108395022No Hit
GTTTTTTACAGGTCCAACAGTCAGAACTACGTAGCTTGGTTCCAGCAGAAACCAGGGCAGCCTCCTAAACTACTC4140.12432619108395022No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC4070.12222405741827956No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC4050.1216234477995165No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATG4050.1216234477995165No Hit
AGTCAGAACTACGTAGCTTGGTTCCAGCAGAAACCAGGGCAGCCTCCTAAACTACTCATTTACTGGGCTTCTACC4040.121323142990135No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCGGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG3960.1189207045150828No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC3920.11771948527755673No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC3890.11681857084941216No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA3880.11651826604003063No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC3880.11651826604003063No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC3770.11321491313683388No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC3720.11171338908992627No Hit
GGTCTCCGGGGTCCCTGACCGATTCAGTGGCAGCGCGTCTGGGACAGATTTCAGTCTCACCATCAGCAGCCTGC3690.1108124746617817No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCGGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCACCTG3670.11021186504301868No Hit
TTATTACTGTCAGCAATATTTTACTACTCCGTACACTTTTGGCCAGGGGA3630.10901064580549258No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT3620.10871034099611104No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGG3590.10780942656796648No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT3580.10750912175858496No Hit
GCCACTGAATCGGTCAGGGACCCCGGAGACCCGGGTAGAAGCCCAGTAAA3560.10690851213982192No Hit
ACGTAGTTCTGACTGTTGGACCTGTAAAAAACACTCTGGCTGGACCTGCAGGTGATGGTGGCCCTCTCGCCCAG3540.10630790252105889No Hit
GGTCCAGCCAGAGTGTTTTTTACAGGTCCAACAGTCAGAACTACGTAGCTTGGTTCCAGCAGAAACCAGGGCAGC3530.10600759771167735No Hit
GTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGACTGAAATCTGTC3520.10570729290229582No Hit
GGTTTCTGCTGGAACCAAGCTACGTAGTTCTGACTGTTGGACCTGTAAAAAACACTCTGGCTGGACCTGCAGGTG3470.10420576885538822No Hit
GTCTGGGACAGATTTCAGTCTCACCATCAGCAGCCTGCAGGCTGAAGATG3460.10390546404600669No Hit
GTTTATTACTGTGCGAGAGGAGGGACAAATTTTCGGGGGAAGACGAACGACAACGACGAATTGGGCTACTGGGGC3430.10300454961786212No Hit
GGTCCAACAGTCAGAACTACGTAGCTTGGTTCCAGCAGAAACCAGGGCAGCCTCCTAAACTACTCATTTACTGGG3420.10270424480848062No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC3380.10150302557095452No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT3360.10090241595219149No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG3360.10090241595219149No Hit
AAAGTGTACGGAGTAGTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTG3350.10060211114280995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)