FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996942
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40790.40915118432165565No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT40150.40273155308934727No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30960.3105496608629188No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26600.2668159230928178No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT25790.25869107731442753No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25270.253475126938177No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG24750.2482591765619264No Hit
GGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACTTAAATAGGTGCTA23670.23742604885740598No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA23230.23301255238519394No Hit
TCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTGTCTGGGACAGAATTCACT22980.23050488393507343No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21520.21586010018636992No Hit
GTAATAGACAGCCGTGTCTTCAGATCTCAGACTGCTCAGTTCCATGTAGGCCGTGGTCGCGGATGTGTTCCTGGT21230.21295120478423019No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG21190.21254997783221088No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCATTAGCACCTATTTAAGTTGGTATCAGCAGCA19540.19599936606141583No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19450.19509660541937243No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCATTAGCAC19340.19399323130131943No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG19290.19349169761129534No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC18940.1899809617811267No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA18910.1896800415671122No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC18680.18737298659300142No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18440.18496562488088575No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGTAT18210.18265856990677493No Hit
GTGGATGCACTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACTTAAATAGGTGCTAATG18190.1824579564307653No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGTATACTGAAACTCTGTTGAC17730.17784384648254362No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC17630.17684077910249543No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTG17520.17573740498444243No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGT17450.1750352578184087No Hit
GAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTAT17420.17473433760439425No Hit
GTCTATTACTGTGCGAGAGAAGTGTGGGAAAAAGGCAACTGGTTCGACCC17130.17182544220225449No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17050.17102298829821594No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16970.1702205343941774No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16540.1659073446599702No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGCGAGAGTGA16530.16580703792196538No Hit
GTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATT16380.1643024368518931No Hit
ACTTACTACTGTCAACAGAGTTTCAGTATACCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGA16160.16209568861578708No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15810.1585849527856184No Hit
CATCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTGTCTGGGACAGAATTC14950.14995857331720402No Hit
CTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACT14460.1450435431549679No Hit
CACTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAA14220.14263618144285223No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC14190.1423352612288378No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC14160.14203434101482332No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC13890.13932605908869322No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGA13770.1381223782326354No Hit
CCCCTAACCTCCTCATCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCA13700.13742023106660167No Hit
GTCCCAGACACACTGCCTGTGAACCTTGATGGGACCCCACTTTGCAAAGTGGATGCACTATGGATGAGGAGGTT13600.1364171636865535No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13510.1355144030445101No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13260.13300673459438964No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13140.13180305373833182No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCC12760.12799139769414872No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12610.12648679662407644No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12360.12397912817395595No Hit
GTTCACAGGCAGTGTGTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTT12340.12377851469794632No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC12190.12227391362787404No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12110.1214714597238355No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11970.12006716539176804No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC11910.11946532496373913No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT11800.11836195084568611No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT11770.11806103063167164No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11660.11695765651361864No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC11560.11595458913357046No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT11560.11595458913357046No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT11090.11124017244734398No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA11080.11113986570933916No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC11030.11063833201931507No Hit
CCTTGGTCCCTTGGCCGAACGTTCGAGGTATACTGAAACTCTGTTGACAG10980.11013679832929098No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10920.10953495790126207No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTATACCTCGAACGTTCGGCCAAGGG10760.10793005009318496No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC10490.10522176816705486No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC10350.10381747383498739No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA10340.10371716709698256No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG10280.10311532666895368No Hit
ACATGGAACTGAGCAGTCTGAGATCTGAAGACACGGCTGTCTATTACTGTGCGAGAGAAGTGTGGGAAAAAGGCA10230.10261379297892956No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10170.10201195255090066No Hit
GTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATC10030.10060765821883319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)