FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996942
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38130.38246959201237385No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT32850.3295076343458295No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29470.2956039569002008No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26970.2705272723989961No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23160.23231040521916022No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA23120.23190917826714091No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT22840.229100589603006No Hit
TCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTGTCTGGGACAGAATTCACT21780.2184680753744952No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG21760.2182674618984856No Hit
GGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACTTAAATAGGTGCTA21460.215258259758341No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20610.20673218702793145No Hit
GTGGATGCACTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACTTAAATAGGTGCTAATG20170.2023186905557194No Hit
GTAATAGACAGCCGTGTCTTCAGATCTCAGACTGCTCAGTTCCATGTAGGCCGTGGTCGCGGATGTGTTCCTGGT19870.1993094884155748No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCATTAGCACCTATTTAAGTTGGTATCAGCAGCA18990.1904824954711508No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18740.1879748270210303No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCATTAGCAC18590.18647022595095802No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18270.18326041033480384No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG17570.17623893867446652No Hit
GTCTATTACTGTGCGAGAGAAGTGTGGGAAAAAGGCAACTGGTTCGACCC17270.17322973653432194No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTG16980.1703208411321822No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC16970.1702205343941774No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC16760.1681140928960762No Hit
GTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATT16740.16791347942006657No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC16670.1672113322540328No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16580.16630857161198948No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16450.1650045840179268No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16370.16420213011388826No Hit
GAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTAT16360.16410182337588344No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGCGAGAGTGA16340.1639012098998738No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGTATACTGAAACTCTGTTGAC16220.16269752904381599No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGTAT15980.16029016733170035No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG15820.15868525952362325No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGT15710.15758188540557022No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC15280.15326869567136303No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15160.1520650148153052No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC15090.15136286764927145No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14950.14995857331720402No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA14720.14765151834309317No Hit
ACTTACTACTGTCAACAGAGTTTCAGTATACCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGA14360.1440404757749197No Hit
CATCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTGTCTGGGACAGAATTC13860.13902513887467877No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13720.1376208445426113No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13650.13691869737657758No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13650.13691869737657758No Hit
CACTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAA13390.1343107221884523No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGA13330.1337088817604234No Hit
GTCCCAGACACACTGCCTGTGAACCTTGATGGGACCCCACTTTGCAAAGTGGATGCACTATGGATGAGGAGGTT13310.13350826828441373No Hit
CTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACT13180.1322042806903511No Hit
CCCCTAACCTCCTCATCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCA13090.13130152004830772No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC12870.12909477181220172No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT12330.1236782079599415No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12030.12066900581979693No Hit
GTTCACAGGCAGTGTGTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTT11930.11966593843974875No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT11890.11926471148772948No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC11840.11876317779770539No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCC11540.1157539756575608No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC11500.11535274870554155No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11230.11264446677941144No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11210.11244385330340179No Hit
GTGGGGTCCCATCAAGGTTCACAGGCAGTGTGTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTG11140.11174170613736807No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT11100.11134047918534878No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC11070.11103955897133434No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11020.11053802528131025No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10880.1091337309492428No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTATACCTCGAACGTTCGGCCAAGGG10540.10572330185707896No Hit
CCTTGGTCCCTTGGCCGAACGTTCGAGGTATACTGAAACTCTGTTGACAG10440.10472023447703077No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC10350.10381747383498739No Hit
ATATAATACATTGGGTGCGCCAGGCCCCCGGACAAAGGCCTGAGTGGATGGGATGGATGAACGCTGGCAATGGTC10240.10271409971693438No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC10190.10221256602691028No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA10090.1012094986468621No Hit
ATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACTTAAATAGGTGCTAATGCTCTGATTTGC10050.10080827169484284No Hit
GTGTGTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC10010.10040704474282354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)