FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849644
Sequences flagged as poor quality0
Sequence length18-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35950.4231183884073801No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT35450.4172335707661091No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT26420.31095376416475606No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGG24970.29388779300507034No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24110.28376590666208434No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22920.2697600406758595No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22860.269053862558907No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21960.2584611908046193No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC20650.2430429685844895No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20410.24021825611667946No Hit
GTAGGAGACAGCGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAA20130.23692275823756775No Hit
ATATTACTGTCAACAATATGATAGTCTCTTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCCAACGAACTG19890.23409804576975768No Hit
GTAATATACAGCCGTGTCCTCAACTCTCAGGCTGTTCATTTGCAGATACA19850.23362726035845602No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19260.22668317554175632No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17200.20243772685972009No Hit
ATATGATAGTCTCTTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCCAACGAACTGTGGCTGCACCA17030.20043688886168795No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16740.1970236946297508No Hit
ATCATATTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGC16740.1970236946297508No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16630.1957290347486712No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16410.193139714986512No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT16310.1919627514582578No Hit
CAATATGATAGTCTCTTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCCAACGAACTGTGGCTGCACCA15660.1843124885246056No Hit
ACATATTACTGTCAACAATATGATAGTCTCTTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCCAACGAA15310.18019311617571593No Hit
AGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTACGA15260.17960463441158886No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGCGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA15120.177956885472033No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14530.1710128006553333No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC14440.16995353347990452No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14160.1666580356007928No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA14000.1647748939555861No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13850.16300944866320483No Hit
GTGCAGCCACAGTTCGTTGGATATCCACTTTGGTCCCAGGGCCGAAAGTG13710.16136169972364894No Hit
GTATATTACTGTGCAAGAGATCGCTACTATGGTTCGGGGACTTATTATCAAACAAACTACTACTACTACGGTA13420.15794850549171183No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA13380.15747772008041014No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC12770.1502982425580596No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12650.1488858863241546No Hit
CTGTAGGAGACAGCGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGC12240.14406033585831243No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12160.14311876503570906No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12010.1413533197433278No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACA11870.13970557080377194No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11820.13911708903964484No Hit
GATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA11340.13346766410402475No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11220.1320553078701197No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGA11160.1313491297531672No Hit
CTATCATATTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC11130.13099604069469095No Hit
GTCTGCATCTGTAGGAGACAGCGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTA10850.12770054281557924No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT10790.1269943646986267No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10740.12640588293449964No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10730.1262881865816742No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA10380.12216881423278456No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10330.12158033246865746No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATATCCACTTTGGTCC10310.12134493976300662No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10280.12099185070453036No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10240.1205210652932287No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10170.11969719082345076No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT10100.11887331635367285No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10060.11840253094237115No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9980.11746096011976781No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG9970.11734326376694239No Hit
GATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAATATGATAGTC9900.11651938929716446No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG9890.11640169294433904No Hit
CCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATC9810.11546012212173568No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATG9690.11404776588783067No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9430.11098766071436979No Hit
GTGGATATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATC9360.11016378624459185No Hit
GCCTGAAGATATTGCAACATATTACTGTCAACAATATGATAGTCTCTTATTCACTTTCGGCCCTGGGACCAAAG9360.11016378624459185No Hit
CATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATAT9330.1098106971861156No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9240.10875143001068684No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9190.10816294824655974No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8990.10580902119005137No Hit
GTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATA8920.10498514672027343No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8860.1042789686033209No Hit
CTTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCCAACGAACTGTGG8770.10321970142789215No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATGCATCGTAGATCAGGAGCTTAGGGGCTCTCCCTGGTTTC8600.10121886342986004No Hit
ATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGAC8540.10051268531290752No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8500.10004189990160586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)