FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004717588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603135
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57720.3600445377338777No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA55240.3445748486559148No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT50080.3123879149291856No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTC48550.3028441148125392No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGAAGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGG44180.27558502559048365No Hit
ATTATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA40830.25468846977952575No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT40450.25231811419499917No Hit
TTATTATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT40280.25125769195981623No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39560.24676649190492378No Hit
GTCTATGGTGGGTCCTTCAGTGGTTTCTACTGGACCTGGATGCGCCAGCC38700.24140200295046893No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG38560.24052871405090653No Hit
GTATAATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT37300.23266911395484471No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA37230.23223246950506352No Hit
CTCCTAAGCTGCTCATTTCCTGGGCGTCTACCCGGGAATCCGGGGTCCCT34630.21601424708461858No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG34180.2132072470503108No Hit
GAGTATAATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT34110.2127706026005296No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT33990.2120220692580475No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32030.19979602466417365No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31740.19798706908650865No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGAGCCGCTGCC29200.18214311333730474No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG29130.1817064688875235No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC28550.17808855773219348No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27410.17097749097861378No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG27000.1684200020584667No Hit
GTGTATTACTGTGCGAGAGGCCTCACGACCGACTTTACGTTTGGGGGAGCGTACTACTACTTCGACCCCTGGGGC26310.16411593533919477No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25970.16199509086882888No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC25850.1612465575263468No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25830.16112180196926645No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC25830.16112180196926645No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC24580.15332457965174487No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24300.15157800185262002No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG24250.15126611295991915No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23910.14914526848955328No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA23100.14409266842779928No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22740.14184706840035305No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22550.14066189060808976No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC21380.13336369051888955No Hit
GGCCAAAAGTGTACGGAGTATAATAATATTGCTGACAGTAATAAACTGCC21360.13323893496180922No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG21220.13236564606224677No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20840.1299952904777202No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20610.12856060157129626No Hit
CAGTAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGA20140.12562884597990812No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGTATAATAATATTGCTGAC20030.12494269041596621No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19750.12319611261684138No Hit
GTTTATTACTGTCAGCAATATTATTATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA19740.1231337348383012No Hit
GAGTGAAATCTGTCCCAGAGCCGCTGCCACTGAATCGGTCAGGGACCCCG19320.12051386813961396No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGAGCCGCTGCCACTGAATCGGTCAGGGACC19220.1198900903542122No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGAGCCGCTGCC18970.11833064589070788No Hit
GTCAGGGACCCCGGATTCCCGGGTAGACGCCCAGGAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGT18920.11801875699800704No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC18720.11677120142720358No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT18580.11589791252764114No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC18560.11577315697056081No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAACTGCAAGTCCAGCCAGAGTGTTTTAT18350.11446322362121718No Hit
GTCCCTGACCGATTCAGTGGCAGCGGCTCTGGGACAGATTTCACTCTCAC17380.10841257910282041No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC17310.1079759346530392No Hit
GTCCCAGAGCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGACGCCCAGGAAATGAGCAGCTT17040.10629173463245452No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17030.10622935685391437No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17010.10610460129683401No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATTATACT16510.10298571236982539No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC16440.10254906792004416No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACTCTGGCTGGACTTGC16400.10229955680588346No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16280.10155102346340139No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16280.10155102346340139No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)