FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604038
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAAACAGCCGTGTCCTCGGCTCTCAGGCCGCTCATTTCCAGAAATACGGAGTTCTTGGCGTTGTCTCTGG35250.5835725566934531No Hit
GTATATGGGCCTACCACTACCACTAATATATGAAATCCACTCCAGCCCCTTCCCTGGCGCCTGGCGGACCCAAAT24850.4113979584065903No Hit
CCCATATACTATGCAGACTCTGTGACGGGCCGATTCACCGTCTCCAGAGA17940.2970011820448382No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC14730.24385883007360465No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG13220.218860402822339No Hit
GCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAACGGCCATCACTCAGGCCCGCATCC12730.2107483304030541No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11170.18492214066002471No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA10810.1789622507193256No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTCTAGAAGGTGTCCAGTGTGAAGTACAGCTGGCGGAGT9970.1650558408576944No Hit
CTCCTGTGCAGCCTCCGGATTCACCATAAGCACTTATGAAATGATTTGGGTCCGCCAGGCGCCAGGGAAGGGG9890.16373142087087236No Hit
GTTTACTACTGTGCGAGGCTGCGGCTGGCGGCTCTTGACTTCTGGGGCCA9730.16108258089722832No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9610.15909595091699527No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9610.15909595091699527No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC9550.1581026359268788No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC9230.1528049559795907No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT9190.15214274598617966No Hit
CTCCAGACTGGGGACGAGGCCGATTATTACTGCGGGACATGGGATGCGGG8870.14684506603889158No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8870.14684506603889158No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8780.1453550935537168No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT8630.14287180607842553No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC8530.141216281094898No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC8460.14005741360642873No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8360.1384018886229012No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8320.1377396786294902No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGCTGATGTCCAGGGAGAATGACGTGCCAGACTTGGAGCCAG8280.1370774686360792No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTC7770.1286342912200888No Hit
GTGCTGGGTTTTCCTTGTTGCTATTCTAGAAGGTGTCCAGTGTGAAGTAC7750.1283031862233833No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7640.12648210874150304No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG7390.12234329628268421No Hit
GATTATTACTGCGGGACATGGGATGCGGGCCTGAGTGATGGCCGTTGGGT7320.12118442879421494No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7320.12118442879421494No Hit
GGACAGAAGGTCAGCATCTCCTGCTCTGGAGGAAAAAATTATATTTCCTGGTATCAGAAACTCCCAGGAGCAGCC7310.1210188762958622No Hit
GTATCAACGCAGAGTACGGGAGCTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATTTCCACTTGGTGATC7230.11969445630904017No Hit
CTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATTTCCACTTGGTGATCAGCACCGAACACAGAGGACTCACC7210.11936335131233465No Hit
GTCTGGGGGAGGCTTGGTACAGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCCGGATTCACCATAAGC7170.11870114131892365No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7090.11737672133210163No Hit
ATACTATGCAGACTCTGTGACGGGCCGATTCACCGTCTCCAGAGACAACG7010.11605230134527961No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG6870.11373456636834106No Hit
CTCTGTGACGGGCCGATTCACCGTCTCCAGAGACAACGCCAAGAACTCCGTATTTCTGGAAATGAGCGGCCTG6810.11274125137822455No Hit
AGTCTGGAGTCCGCTGATGTCCAGGGAGAATGACGTGCCAGACTTGGAGCCAGAGAATCGATCAGGAATCCCTG6780.1122445938831663No Hit
CTCCTGCTCTGGAGGAAAAAATTATATTTCCTGGTATCAGAAACTCCCAG6770.11207904138481355No Hit
GTTTGGGGGCTGCTCCTGGGAGTTTCTGATACCAGGAAATATAATTTTTT6690.11075462139799153No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC6550.10843688642105298No Hit
GAGTACGGGAGCTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATTTCCACTTGGTGATCAGCACCGAAC6460.10694691393587821No Hit
ATTCTAGAAGGTGTCCAGTGTGAAGTACAGCTGGCGGAGTCTGGGGGAGGCTTGGTACAGCCTGGAGGGTCCCTG6410.10611915144411443No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC6330.10479473145729241No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC6310.10446362646058692No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATG6310.10446362646058692No Hit
GCCCCCAAACTCCTCATTTATGACGATGATAAGCGACCCTCAGGGATTCCTGATCGATTCTCTGGCTCCAAGT6310.10446362646058692No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG6180.10231144398200112No Hit
CTATGCAGACTCTGTGACGGGCCGATTCACCGTCTCCAGAGACAACGCCAAGAACTCCGTATTTCTGGAAATG6150.10181478648694288No Hit
CCCTCAATGTCTGCGGCCCCAGGACAGAAGGTCAGCATCTCCTGCTCTGGAGGAAAAAATTATATTTCCTGGTAT6120.10131812899188462No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6110.10115257649353188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)