FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604038
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAAACAGCCGTGTCCTCGGCTCTCAGGCCGCTCATTTCCAGAAATACGGAGTTCTTGGCGTTGTCTCTGG31610.5233114472930511No Hit
GTATATGGGCCTACCACTACCACTAATATATGAAATCCACTCCAGCCCCTTCCCTGGCGCCTGGCGGACCCAAAT23620.3910350011092017No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20040.3317672066989163No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC15630.2587585549253524No Hit
CCCATATACTATGCAGACTCTGTGACGGGCCGATTCACCGTCTCCAGAGA14320.2370711776411418No Hit
GCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAACGGCCATCACTCAGGCCCGCATCC11700.1936964230727206No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10960.1814455381946169No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10250.16969131081157146No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA9920.16422807836593062No Hit
CTCCTGTGCAGCCTCCGGATTCACCATAAGCACTTATGAAATGATTTGGGTCCGCCAGGCGCCAGGGAAGGGG9870.16340031587416687No Hit
GTCCTGGGCCCAGTCTTTATTGACGCAGCCGCCCTCAATGTCTGCGGCCCCAGGACAGAAGGTCAGCATCTCCTG9790.16207589588734483No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC9680.16025481840546454No Hit
GTTTACTACTGTGCGAGGCTGCGGCTGGCGGCTCTTGACTTCTGGGGCCA8830.1461828560454806No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTCTAGAAGGTGTCCAGTGTGAAGTACAGCTGGCGGAGT8510.1408851760981925No Hit
GTGCTGGGTTTTCCTTGTTGCTATTCTAGAAGGTGTCCAGTGTGAAGTAC8450.139891861108076No Hit
CTCCAGACTGGGGACGAGGCCGATTATTACTGCGGGACATGGGATGCGGG8270.13691191613772644No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8260.13674636363937367No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG8250.1365808111410209No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8240.13641525864266818No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT8050.13326976117396588No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC7920.1311175786953801No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC7770.1286342912200888No Hit
GTATCAACGCAGAGTACGGGAGCTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATTTCCACTTGGTGATC7770.1286342912200888No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC7660.12681321373820853No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7430.12300550627609522No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7370.12201219128597869No Hit
GTCTTTATTGACGCAGCCGCCCTCAATGTCTGCGGCCCCAGGACAGAAGGTCAGCATCTCCTGCTCTGGAGGAAA6970.11539009135186859No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG6970.11539009135186859No Hit
CTCCTGCTCTGGAGGAAAAAATTATATTTCCTGGTATCAGAAACTCCCAG6950.11505898635516308No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6910.11439677636175208No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC6890.11406567136504657No Hit
CTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATTTCCACTTGGTGATCAGCACCGAACACAGAGGACTCACC6830.11307235637493006No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6570.10876799141775848No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6570.10876799141775848No Hit
GAGTACGGGAGCTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATTTCCACTTGGTGATCAGCACCGAAC6560.10860243891940573No Hit
GGGTTCCCTGGCCCCAGAAGTCAAGAGCCGCCAGCCGCAGCCTCGCACAGTAGTAAACAGCCGTGTCCTCGGCT6470.10711246643423096No Hit
CTCTGTGACGGGCCGATTCACCGTCTCCAGAGACAACGCCAAGAACTCCGTATTTCTGGAAATGAGCGGCCTG6460.10694691393587821No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC6440.1066158089391727No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6360.10529138895235068No Hit
AGTCTGGAGTCCGCTGATGTCCAGGGAGAATGACGTGCCAGACTTGGAGCCAGAGAATCGATCAGGAATCCCTG6130.10148368149023737No Hit
GATTATTACTGCGGGACATGGGATGCGGGCCTGAGTGATGGCCGTTGGGT6070.10049036650012086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)