FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749270
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCCCTGG31220.416672227634898No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTTTGATGTACACTGGTACCAGCACCTTCCAGGAACAGC28300.37770096226994276No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26730.3567472339744018No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGAACCTGACTCAGGCTGCCGCCATA25690.34286705726907524No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGAACCTGACTCAGGCTGCCGCCATAAGACTGGCAGTAAT25410.33913008661764116No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24670.32925380703885115No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23580.31470631414576855No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA22890.3054973507547346No Hit
GTGTTAGTATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGGTGCTGGTACCAGTGTACATCAAAACCTGCCCCG20450.2729323207922378No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19500.2602533132248722No Hit
GATTATTACTGCCAGTCTTATGGCGGCAGCCTGAGTCAGGTTCTCTTCGGCGGAGGGACCAAGCTGACCGTCCT19270.2571836587611942No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18180.24263616586811163No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTATAACTTCTGTGGGACTTCCACTGCTCAGGCGTC17860.23836534226647269No Hit
GTCTTATGGCGGCAGCCTGAGTCAGGTTCTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG17640.23542915104034592No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17000.22688750383706807No Hit
CCATAAGACTGGCAGTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC16960.2263536508868632No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTATAACTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT16940.22608672441176078No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC16520.22048126843460966No Hit
CATCTATACTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTC16490.22008087872195603No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCAGAGAATCGGTC16280.2172781507333805No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC16130.21527620217011226No Hit
GGCCATCACTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCCAGT15950.21287386389419036No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15490.20673455496683438No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG15260.2036649005031564No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14980.19992792985172236No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14790.19739212833824923No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14770.1971252018631468No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14700.19619095920028828No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC14580.19458940034967367No Hit
CTTCATGCGTGACCTGGCAGCTATAACTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14350.19151974588599568No Hit
GCTATAACTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14250.19018511351048353No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG13770.18377887810802515No Hit
TCACTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCCAGTCTTAT13680.1825777089700642No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCAG13150.1755041573798497No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATACTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGC12960.17296835586637663No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCATCTATACTAACACCAATCGGCCCTC12880.17190064996596688No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTTTGATGTACACTGGTACC12360.16496056161330364No Hit
CCCTTCATGCGTGACCTGGCAGCTATAACTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12330.16456017190064998No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTATAACTT12230.1632255395251378No Hit
GTAATAGACGGCCGTGTCGTCAGATCTGAGCCCGCTCAGTTCCATGTAGACTGTGGCGGTGGACGTGTCGCGGGT12230.1632255395251378No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG12110.16162398067452322No Hit
GTTCCTGGAAGGTGCTGGTACCAGTGTACATCAAAACCTGCCCCGATGTT11700.15615198793492333No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC11640.15535120850961603No Hit
GTATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGGTGCTGGTACCAGTG11560.15428350260920629No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA11530.15388311289655265No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCGCTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT11510.15361618642145022No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11420.15241501728348927No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11350.15148077462063075No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCT11340.15134731138307952No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT11260.1502796054826698No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGAACCTGACTCAGGCTGCCGCCATAAGACTGGCAGTAATA10920.14574185540592843No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGAAC10800.14414029655531382No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG10710.14293912741735287No Hit
GTCTATTACTGTGCGAGAGACATGGGATATGGTACTACCATTTCAGGCATCGATCTTTGGAACAAGTACTTTG10360.1382679141030603No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGACGATG9870.13172821546305072No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTATAACTTCTGT9720.12972626689978245No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC9700.12945934042468002No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGAACCTGACTC9510.1269235389112069No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA9490.12665661243610446No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9130.12185193588426069No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT9100.12145154617160703No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTATAACTTCTGTGGGACTTCC9050.12078422998385095No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC8800.11744764904507053No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG8770.11704725933241687No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8700.11611301666955838No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8630.11517877400669986No Hit
GTGCTGGTACCAGTGTACATCAAAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTG8590.11464492105649499No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGTTATAGCTGC8590.11464492105649499No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCGCTCTCCTCGCTCACTGCACAGGGT8540.11397760486873891No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG8460.11290989896832918No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8440.11264297249322675No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8440.11264297249322675No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCC8280.11050756069240728No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTTTGATGT8190.10930639155444632No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8150.10877253860424148No Hit
GTTTGGGGGCTGTTCCTGGAAGGTGCTGGTACCAGTGTACATCAAAACCT8130.10850561212913903No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8110.1082386856540366No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC8100.10810522241648539No Hit
GCTCCAACATCGGGGCAGGTTTTGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCATCT8080.10783829594138296No Hit
GTGCCAGACTTGGAGGCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT7970.10637020032831956No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGC7910.10556942090301227No Hit
GCTTGGTCCCTCCGCCGAAGAGAACCTGACTCAGGCTGCCGCCATAAGACTGGCAGTAATAATCAGCCTCATCGT7780.10383439881484645No Hit
CCCCTGAGGGCCGATTGGTGTTAGTATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGGTGCTGGTACCAGTGT7780.10383439881484645No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAG7660.10223283996423184No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCGCTCTC7650.10209937672668062No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT7600.10143206053892455No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCGCTCTCCTCGCTCACTGCACAGGGTCCTGGGC7580.1011651340638221No Hit
CTCCTCATCTATACTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTC7570.10103167082627089No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC7560.10089820758871967No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC7560.10089820758871967No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7500.1000974281634124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)