FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749270
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCCCTGG31480.4201422718112296No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27770.37062741067972826No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTTTGATGTACACTGGTACCAGCACCTTCCAGGAACAGC26640.3555460648364408No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23680.3160409465212807No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGAACCTGACTCAGGCTGCCGCCATA23640.3155070935710758No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA22670.30256115952860785No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGAACCTGACTCAGGCTGCCGCCATAAGACTGGCAGTAAT21840.29148371081185687No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20400.27226500460448166No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20000.266926475102433No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG19970.26652608538977934No Hit
GATTATTACTGCCAGTCTTATGGCGGCAGCCTGAGTCAGGTTCTCTTCGGCGGAGGGACCAAGCTGACCGTCCT18160.2423692393930092No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC17910.23903265845422877No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTATAACTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT17160.22902291563788754No Hit
GTCTTATGGCGGCAGCCTGAGTCAGGTTCTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16820.22448516556114617No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTATAACTTCTGTGGGACTTCCACTGCTCAGGCGTC16430.2192800992966487No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16120.21514273893256103No Hit
CATCTATACTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTC15900.21220654770643427No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC15840.21140576828112698No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15800.2108719153309221No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15380.205266459353771No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14910.19899368718886384No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14780.19725866510069803No Hit
GTAATAGACGGCCGTGTCGTCAGATCTGAGCCCGCTCAGTTCCATGTAGACTGTGGCGGTGGACGTGTCGCGGGT14700.19619095920028828No Hit
GGCCATCACTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCCAGT14690.19605749596273708No Hit
GTGTTAGTATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGGTGCTGGTACCAGTGTACATCAAAACCTGCCCCG14480.19325476797416152No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC14440.19272091502395666No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14310.19098589293579082No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCGCTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT14240.1900516502729323No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG13080.1745699147169912No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12940.17270142939127417No Hit
CTTCATGCGTGACCTGGCAGCTATAACTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12660.16896445873984012No Hit
GCTATAACTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12540.1673628998892255No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGTTATAGCTGC12340.16469363513820118No Hit
CCATAAGACTGGCAGTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC12260.16362592923779146No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12170.1624247600998305No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCAGAGAATCGGTC12140.16202437038717685No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAACTCCTCATCTATACTAACACCAATCGGCCCTC12070.16109012772431833No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCC12040.1606897380116647No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATACTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGC11980.15988895858635738No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11780.15721969383533307No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC11770.15708623059778185No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11770.15708623059778185No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTTTGATGTACACTGGTACC11600.15481735555941117No Hit
TCACTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCCAGTCTTAT11590.15468389232185994No Hit
CCCTTCATGCGTGACCTGGCAGCTATAACTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11380.15188116433328439No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC11070.1477438039691967No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTATAACTT11020.1470764877814406No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA10900.145474928930826No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT10450.13946908324102128No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG10430.13920215676591882No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT10360.1382679141030603No Hit
GTCTATTACTGTGCGAGAGACATGGGATATGGTACTACCATTTCAGGCATCGATCTTTGGAACAAGTACTTTG10000.1334632375512165No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9630.1285250977618215No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCGCTCTCCTCGCTCACTGCACAGGGT9620.1283916345242703No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGAACCTGACTCAGGCTGCCGCCATAAGACTGGCAGTAATA9540.12732392862386055No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGACGATG9530.12719046538630935No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGAAC9460.12625622272345083No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG9290.12398734768508016No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCGCTCTCCTCGCTCACTGCACAGGGTCCTGGGC9270.12372042120997771No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT9200.1227861785471192No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTATAACTTCTGT9040.12065076674629974No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCGCTCTC8970.11971652408344122No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCAG8930.11918267113323634No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8770.11704725933241687No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCC8660.1155791637193535No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC8540.11397760486873891No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8220.10970678126709997No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTTTGATGT8040.10730444299117808No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8000.10677059004097322No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG7950.10610327385321713No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7930.1058363473781147No Hit
CTCCTCATCTATACTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTC7920.10570288414056349No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC7910.10556942090301227No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCT7890.10530249442790983No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC7870.1050355679528074No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7820.10436825176505131No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7810.1042347885275001No Hit
GCTTGGTCCCTCCGCCGAAGAGAACCTGACTCAGGCTGCCGCCATAAGACTGGCAGTAATAATCAGCCTCATCGT7730.10316708262709036No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGAACCTGACTC7700.10276669291443671No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG7690.1026332296768855No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGC7560.10089820758871967No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA7540.10063128111361727No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTATAACTTCTGTGGGACTTCC7500.1000974281634124No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCGCTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG7500.1000974281634124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)