FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989445
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37840.3824366185083557No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATCTGCGACCATTCAGGCTGTCATCCCATG36690.3708139411488258No Hit
GATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCGCAGATTCGGCGGAGGGACCAAGCTGACCGTCCT36680.37071287438917777No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCGGACTTGGAGGCAG34350.34716431939117387No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34100.3446376503999717No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATCTGCGACCATTCAGGCTGTCATCCCATGCTGAACAGTAAT33610.33968537917721553No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGTCCCCCGGGCAGAGGGTCATCATCTCTTG32920.3327117727614976No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31210.315429356861675No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA29720.3003704096741102No Hit
GTATTAGCTCCGATGTTGGAGCTGCTTCCAGAGCAAGAGATGATGACCCT29180.29491280465311365No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC28140.2844018616497127No Hit
GTTTAGAACCTATGACCATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC26700.2698482482603884No Hit
GAGCTAATACTGTAAACTGGTACCAGCACCTCCCAGGGACGGCCCCCAAA26230.2650981105569284No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT25920.26196504100783774No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC23940.2419538225975168No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23930.24185275583786872No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGTCCCCCGGGC23850.24104422176068402No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC23640.23892181980807423No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22450.22689487540995204No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT21520.21749566676268012No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC21130.21355406313640474No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG21120.21345299637675666No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGTCCCCCGGGCAGAGGGTCATCATCTCTTGCTCTGGAAGCAGCTCC20670.20890499219259284No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20630.20850072515400048No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCGGACTTGGAGGCAGAGAATCGATCAGGGACC20620.2083996583943524No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20460.20678259023998302No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20090.20304312013300385No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCC20030.20243671957511536No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19990.202032452536523No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19760.19970791706461705No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC19600.19809084891024767No Hit
CCCCTGAGGGCCGCTGGAAATTACTGTAGATGAGGAGTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCAGTTT19550.19758551511200725No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC19490.19697911455411873No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18470.186670305070014No Hit
GTCTGAGGATGAGGCTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCGCAGATTCGGCGGAGGGAC18170.18363830228057143No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG17850.1804041659718327No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCCCTCCTCCTCACCCTCCTCACTC16600.167770821015822No Hit
CCCAGGGACGGCCCCCAAACTCCTCATCTACAGTAATTTCCAGCGGCCCTCAGGGGTCCCTGATCGATTCTCTGC16460.1663558863807488No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16140.16312175007201007No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC16030.16201001571588114No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATCTGCGACCATTCAGGCTGTCATCCCATGCTGAACAGTAATA15910.1607972146001041No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA15780.15948334672467898No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15530.15695667773347685No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT14960.151195872433536No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14920.15079160539494363No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCGGACTTGGAGGCAGAGAATCGATC14210.14361586545992958No Hit
GTCCCTGGGAGGTGCTGGTACCAGTTTACAGTATTAGCTCCGATGTTGGAGCTGCTTCCAGAGCAAGAGATGATG14060.14209986406520828No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATCTGCGAC13920.14068492943013508No Hit
CCTCAGGGGTCCCTGATCGATTCTCTGCCTCCAAGTCCGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT13770.13916892803541378No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGACCATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC13590.13734972636174825No Hit
GCATGGGATGACAGCCTGAATGGTCGCAGATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG13530.13674332580385973No Hit
CCCTCAGCGTCTGGGTCCCCCGGGCAGAGGGTCATCATCTCTTGCTCTGGAAGCAGCTCCAACATCGGAGCTAAT13240.13381238977406526No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13200.13340812273547292No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA13190.13330705597582482No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCCCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT12690.12825371799342056No Hit
GTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCAGTTTACAGTATTAGCTCCGATGTTGGAGCTGCTTCCAGAGC12680.12815265123377245No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12660.1279505177144763No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGACCATTCT12570.12704091687764352No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCG12540.12673771659869926No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCCCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC12280.12410998084784904No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATCTGCGACCATTC12270.12400891408820096No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12200.12330144677066435No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCCCTCCTCCTCACCCTC12060.12188651213559117No Hit
GCTTGGTCCCTCCGCCGAATCTGCGACCATTCAGGCTGTCATCCCATGCTGAACAGTAATAATCAGCCTCATCCT11980.12107797805840648No Hit
ACCTATGACCATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11980.12107797805840648No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11600.11723744119177923No Hit
ATCCCATGCTGAACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC11530.11652997387424262No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCCCTCCTCCTCACCCTCCTC11420.11541823951811368No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11380.11501397247952136No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11330.11450863868128092No Hit
GGATGACAGCCTGAATGGTCGCAGATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC11320.11440757192163283No Hit
GAACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAG11280.11400330488304049No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11240.11359903784444814No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCCCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT11210.11329583756550389No Hit
GGTTTAGAACCTATGACCATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC11160.11279050376726346No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGTCCCCCGGGCAGAGGGTCATCATCTCTTGCTCTGGAAGC11150.11268943700761538No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11140.1125883702479673No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA11100.11218410320937494No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC11090.11208303644972686No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11060.11177983617078262No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG11000.11117343561289411No Hit
TTACTGTTCAGCATGGGATGACAGCCTGAATGGTCGCAGATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTC10940.11056703505500558No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10910.11026383477606133No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG10820.10935423393922857No Hit
ATGCTGAACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG10780.10894996690063621No Hit
TCCCTGGGAGGTGCTGGTACCAGTTTACAGTATTAGCTCCGATGTTGGAGCTGCTTCCAGAGCAAGAGATGATG10690.10804036606380345No Hit
GTTCAGCATGGGATGACAGCCTGAATGGTCGCAGATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCA10640.10753503226556302No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGTCCCCCGGGCAGAGGGTCATCATCTCTTGCTCTGG10560.10672649818837833No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAG10530.10642329790943408No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCCCTC10520.106322231149786No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10430.10541263031295321No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC10410.10521049679365704No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC10210.10318916160069534No Hit
CCTATGACCATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10130.10238062752351065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)