FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465811
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT22340.4795936549373029No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22170.47594410608594473No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGT19840.4259238188879181No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15860.3404814398972974No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT14090.30248319597433293No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13800.29625749499260434No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG13780.29582813630420923No Hit
GTACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTC12600.2704959736888996No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG12520.2687785389353193No Hit
GTCTGGGACAGAGTTCACTCTTACCATCAGCAGCCTGCAGTCTGAAGATT12470.26770514221433156No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11950.2565418163160595No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTC11760.2524629087763063No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC11720.25160419139951606No Hit
GAGTTCACTCTTACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCTCTATAGTAATTGG11620.24945739795754074No Hit
GTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTTACAGCAACTTAGCCTGGT11580.24859868058075058No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGGT11510.24709592517136778No Hit
CTATAGAGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGAACTCTGTCCC11130.23893811009186128No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA11090.23807939271507111No Hit
TTACTATAGAGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGAACTCTGTC10670.22906286025877448No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC10630.22820414288198432No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG10490.22519863206321875No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10480.22498395271902125No Hit
CTATAGTAATTGGCCCGCGCTCACTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACCAT10440.22412523534223108No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA10170.21832889304889752No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTC10010.2148940235417369No Hit
CTCCTGCAGGACCAGTCAGAGTGTTTACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT10000.21467934419753934No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC9810.21060043665778608No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9730.20888300190420578No Hit
GCTCTATAGTAATTGGCCCGCGCTCACTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACC9630.2067362084622304No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGGTGGATGCACCAT9610.20630684977383532No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGAACTCTGTCCCAGACCCACTGCC9550.20501877370865004No Hit
GTCTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTTAC8890.19084993699161248No Hit
GTTTATTACTGTCAGCTCTATAGTAATTGGCCCGCGCTCACTTTCGGCGGAGGGACCACGGTGGAGATCAAACGA8810.18913250223803216No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8800.18891782289383463No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCC8680.18634167076346414No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8570.1839801979772912No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7890.16938200257185854No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGTGAGCGCGGGCCA7860.16873796453926593No Hit
AGATAGTACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCT7750.166376491753093No Hit
GTCAGAGTGTTTACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG7670.16465905699951267No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGTGAGCGCGGGCCAATTACTATAG7650.16422969831111758No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7630.1638003396227225No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC7570.16251226355753728No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGG7550.1620829048691422No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7510.16122418749235204No Hit
CTCTTACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCTCTATAGTAATTGGCCCGCG7450.15993611142716682No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7160.15371041044543815No Hit
TCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT6980.14984618224988247No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6690.1436204812681538No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT6620.14211772585877105No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTACTGGAGAAAT6590.14147368782617842No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6580.1412590084819809No Hit
CCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTTACAGCAACTTAGC6550.14061497044938828No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC6550.14061497044938828No Hit
ATAGTAATTGGCCCGCGCTCACTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACCAT6520.13997093241679565No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6460.1386828563516104No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT6380.1369654215980301No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC6370.13675074225383257No Hit
CTGCAGGACCAGTCAGAGTGTTTACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCT6330.13589202487704238No Hit
TGGTAAGAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGGTGGATGCAC6300.1352479868444498No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC6250.13417459012346208No Hit
CACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTTACCATCAGC6190.13288651405827687No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGTAAACACTCTGACTGGTCCTGCAGGAGAGGGTGGCTGTTTCCC6080.13052504127210393No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6070.1303103619279064No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6010.12902228586272113No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT5930.12730485110914083No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTT5740.12322594356938758No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC5700.12236722619259743No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC5680.12193786750420235No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA5670.1217231881600048No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC5570.11957639471802942No Hit
GTTTACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACC5470.11742960127605402No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5400.11592684586667125No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCC5380.11549748717827617No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5300.11378005242469585No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG5300.11378005242469585No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC5240.11249197635951062No Hit
GCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACC5210.111847938326918No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC5180.11120390029432536No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5180.11120390029432536No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC5090.10927178619654754No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCA5060.1086277481639549No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5020.10776903078716475No Hit
GCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTG4990.10712499275457213No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGTAAACACTCTGACTGGTCCTGCAGGAG4970.10669563406617705No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC4960.10648095472197952No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT4940.10605159603358444No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC4910.10540755800099182No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTTACCATCAGCAGCC4890.10497819931259673No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC4860.10433416128000414No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTACTGGAGAAATAGTGATGACGCAGTCTC4820.10347544390321396No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTC4820.10347544390321396No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC4810.10326076455901642No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC4800.10304608521481888No Hit
CCAGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTTACAGCA4740.10175800914963365No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTACTGGAGAAATAGTGATGACG4730.10154332980543612No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT4730.10154332980543612No Hit
GGCCAATTACTATAGAGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGA4690.10068461242864594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)