FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004717610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611221
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATACTGCTGACAGAAATACACTGCAAAATCTTCGGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC38820.2409352906894833No Hit
GTATAACATGACGCCGCTCACTTTCGGCGGAGGGACCCAGGTGGAGATCAAACGAACTGTGGCTGCACCAT35170.21828166340930266No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33810.20984085981997505No Hit
ATAATAAACAGCCGTGTCGGCGGCGGTCACAGAGCTCAGGCTCAGGGAGA31190.19357989996406452No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC31090.19295925264131983No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30400.18867678611438157No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG30030.18638039102022627No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT29930.1857597436974816No Hit
GTTTATTATTGTGCGAGAACATATGATACTAGTGGCTACTCTTACGCCACTGATCCTTTTGATATTTGGGGCCA29670.18414606065834543No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT28990.17992565886368164No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG28470.17669829278540933No Hit
CTCTTATAGTGGGACCACTTTCTACAACCCGTCCCTCAGGGGTCGAGTCA27170.16862987758972853No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG26870.16676793562149453No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26300.16323024588184987No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26130.16217514543318393No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTGCCTCCTGCTACTCTGGCTCCCAGATACC24950.15485150702479672No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG23650.14678309182911592No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC23550.14616244450637125No Hit
CTATAAGAGAAACTCCCAATCCACTCCAGCCCCTTCCCGGGGAACTGGCGGATCCAGCCCCAGTAGTAATTCGT23460.14560386191590105No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA23120.14349366101856914No Hit
GTCTCTGGCGGCTCCATCAGTAGTACGAATTACTACTGGGGCTGGATCCG23090.14330746682174575No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22620.14039042440484578No Hit
CTCTTACGCCACTGATCCTTTTGATATTTGGGGCCAAGGGACAATGGTCACAGTCTCTTCAGCATCCCCGACC22540.13989390654665002No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG22540.13989390654665002No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG21410.13288059179963518No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC21110.13101864983140116No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT20660.12822573687905012No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG20570.12766715428857991No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA20170.1251845649976012No Hit
TCTTTGGGGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGCGGCAGTGGGTCAGGGACAGACTTCACT20020.12425359401348418No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG20010.12419152928120972No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19940.12375707615528844No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC19500.12102622793521187No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACGTGCAGTGTCTCTGGCGGCTCCATCAGTAGTACGAATT19270.11959873909289911No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18990.11786092658921403No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG18810.1167437614082736No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT18750.11637137301462679No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18680.11593691988870553No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC18540.11506801363686298No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC18490.11475768997549064No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18470.1146335605109417No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC18440.11444736631411831No Hit
CCCTGAGCCTGAGCTCTGTGACCGCCGCCGACACGGCTGTTTATTATTGTGCGAGAACATATGATACTAGTGGCT18200.11295781273953107No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC18100.1123371654167864No Hit
GTCCTGTCCCAGCTGCAGCCGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACGTGC17900.11109587077129705No Hit
GATGGGTCCTGTCCCAGCTGCAGCCGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTC17880.11097174130674811No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACTGTGACCATTGTCCCTTGGCCCCAAATATCAAAAGGATCAGTGG17620.10935805826761197No Hit
GTCCCAGCTGCAGCCGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACGTGCAGTGT17570.10904773460623962No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT17560.10898566987396516No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT17500.10861328148031833No Hit
CCCCAAAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCCGCTGTCAACACT17260.10712372790573112No Hit
GGCTCAGGGAGAGGTGATTCTTGGACGTGTCTGCGGATATGGTGACTCGA17140.10637895111843751No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGACAGCGGCTACTTAGCCTGGTACC16950.10519972120522263No Hit
GGGCTGGAGTGGATTGGGAGTTTCTCTTATAGTGGGACCACTTTCTACAACCCGTCCCTCAGGGGTCGAGTCACC16940.10513765647294816No Hit
GTATTTCTGTCAGCAGTATAACATGACGCCGCTCACTTTCGGCGGAGGGA16820.10439287968565453No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16770.1040825560242822No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16700.10364810289836092No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGAC16480.10228267878832265No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16480.10228267878832265No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16250.1008551899460099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)