FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167125
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37000.3170183142336939No Hit
CTATTATGCTGTACACAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC35610.30510870729356326No Hit
GCATAATAGTTACCACATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT33830.2898575559601585No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC26050.22319802934561422No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25410.217714469315626No Hit
CTGTTAGGGTTCATCCATCCCATCCACTCAAGCCCTTGTCCAGTGGCCTG24570.21051729677626646No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23380.20032130234550716No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT21630.1853271928885081No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18880.16176502088465244No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18650.15979436649887546No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18620.15953732462246975No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA17970.15396808396701295No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG17330.14848452393702474No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16790.14385777016172216No Hit
CCCTAACAGTGGTCACACAGGCTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACCTCCATA15370.13169112134518582No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15310.13117703759237442No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15300.13109135696690585No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT15080.1292063832065974No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA15000.12852093820284888No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC14580.12492235193316911No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG14570.12483667130770056No Hit
GATCTATGCCGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTTCGCGGCAGTGGATCTGGGACAGAATTC14180.12149512691442647No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14180.12149512691442647No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG14180.12149512691442647No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT14050.12038127878333511No Hit
GGCTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACCTCCATAAGCACAGCCTACATGGAGTTG14030.12020991753239799No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14010.12003855628146085No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13850.11866766627396379No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCC13770.11798222127021527No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCC13700.11738245689193533No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC13070.11198457748741565No Hit
GTAGGAGACAGCGTCACCATCACTTGCCGGGCAAGTCAGGACATTAGAAATGATTTAGGCTGGTATCAGCACAA12580.10778622683945593No Hit
GTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCG12480.10692942058477027No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT12380.10607261433008461No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACACACATGGAGGACGCATTCTGCTGGA12180.1043590018207133No Hit
GTGCATAGCCTGTGTGACCACTGTTAGGGTTCATCCATCCCATCCACTCAAGCCCTTGTCCAGTGGCCTGTCGC12100.10367355681696476No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC11880.1017885830566563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)