FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269073
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAACTCAGTATCAGGGAGAACTCCTTCTTGGACAGGTCCAC41760.3290590848595786No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT34990.27571305984762107No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33000.2600323228057015No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26120.2058195233843916No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT25130.19801855370022053No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG22360.1761915981192571No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21690.1709121539895656No Hit
GTCTATTACTGTGCGAGGTCCGACCCTATTATTATTTTTGGACCATGGTACTTTGACCACTGGGGCCAGGGAGCC20730.16334757732612704No Hit
ATCTACTATAGTGGGAGCACCTTCTACAACCCCTCACTGAAGAGTCGAGTCACCTTGTCTGTGGACCTGTCCA20280.1598016820151402No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA20260.1596440866679852No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAGCTATAAAAGTGCC19980.1574377518078156No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG19910.15688616809277323No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCC19810.15609819135699837No Hit
CTTTTATAGCTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC19590.1543646425382937No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18950.14932159142933463No Hit
GTCTCTAATGTCTCCAATTTTTTTTATTATTGGAGTTGGATCCGGCAGCC18830.14837601934640482No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT18830.14837601934640482No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC17840.14057504966223378No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17830.14049625198865628No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA17650.13907789386426156No Hit
GTGAATGCACCATAGATGAGGAGCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGCTAAAGAATTGGTAATG17250.13592598692116215No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG17230.13576839157400716No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17150.1351380101853873No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACATCC17130.1349804148382323No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC16880.13301047299879518No Hit
GTCCTGGACCTCCTGTCCAAGAACATGAGACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC16580.13064654279147062No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCGAGATGGGACCCCGGATTGCAAGGTGAATGCACCAT16390.1291493869934984No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16160.12733704050121625No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAGAAGGGCACTTTTATAGCTTTGAC15910.1253670986617791No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15830.12473671727315924No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT15710.12379114519022939No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC15680.12355475216949693No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGG15670.12347595449591947No Hit
CCATAGATGAGGAGCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGC15620.12308196612803204No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15570.1226879777601446No Hit
ACTTATTACTGTCAAAGCTATAAAAGTGCCCTTCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA15330.12079683359428497No Hit
GTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCC15180.11961486849062268No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACAT15160.11945727314346773No Hit
CATCTATGGTGCATTCACCTTGCAATCCGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGAGTTC15100.11898448710200281No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14970.1179601173454955No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACC14930.11764492665118556No Hit
AGTCAGGGCATTACCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAA14720.11599017550605836No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14330.11291706623653643No Hit
GTCCCAGATCCACTGCCACTGAACCGAGATGGGACCCCGGATTGCAAGGTGAATGCACCATAGATGAGGAGCTT14250.11228668484791655No Hit
ATAGTAGATAGACCCAATCCACTCCAGTCCCTTCCCTGGAGGCTGCCGGA14200.11189269648002913No Hit
CATTAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGACTCCTGCAGGTGC13870.10929237325197211No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13860.10921357557839462No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13390.10551008492025282No Hit
GTATCAGGGAGAACTCCTTCTTGGACAGGTCCACAGACAAGGTGACTCGA13330.1050372988787879No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC13310.10487970353163292No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13290.10472210818447795No Hit
GGGCATTACCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTCATCTATGGTGCATT13200.10401292912228059No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC12990.1023581779771534No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG12770.10062462915844872No Hit
GCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGCTAAAGAATTGGTA12700.10007304544340634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)