FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269073
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAACTCAGTATCAGGGAGAACTCCTTCTTGGACAGGTCCAC39280.3095172618123622No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34140.2690152575935348No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT29820.23497466260806116No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28660.22583413247307285No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24550.19344828863272642No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT23070.18178623294325857No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA22720.1790283143680466No Hit
ATCTACTATAGTGGGAGCACCTTCTACAACCCCTCACTGAAGAGTCGAGTCACCTTGTCTGTGGACCTGTCCA21370.16839062843508606No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20490.16145643316026737No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG20450.16114124246595746No Hit
GTCTATTACTGTGCGAGGTCCGACCCTATTATTATTTTTGGACCATGGTACTTTGACCACTGGGGCCAGGGAGCC19670.15499502392691358No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAGCTATAAAAGTGCC19600.1544434402118712No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19470.15341907045536388No Hit
GTCTCTAATGTCTCCAATTTTTTTTATTATTGGAGTTGGATCCGGCAGCC19400.1528674867403215No Hit
CTTTTATAGCTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC19300.15207951000454661No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17790.14018106129434635No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCGAGATGGGACCCCGGATTGCAAGGTGAATGCACCAT17770.14002346594719137No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCC17740.13978707292645892No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT17220.13568959390042967No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC16530.13025255442358322No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16500.13001616140285074No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG16490.12993736372927325No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT16480.1298585660556958No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC16410.12930698234065338No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGG16170.12741583817479374No Hit
GTGAATGCACCATAGATGAGGAGCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGCTAAAGAATTGGTAATG16010.12615507539755397No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC15690.12363354984307443No Hit
GTCCCAGATCCACTGCCACTGAACCGAGATGGGACCCCGGATTGCAAGGTGAATGCACCATAGATGAGGAGCTT15600.12292437078087706No Hit
GTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCC15560.12260918008656713No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACATCC15450.1217424056772148No Hit
GTCCTGGACCTCCTGTCCAAGAACATGAGACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC15320.12071803592070748No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15210.11985126151135514No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC14870.11717214060972063No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA14850.11701454526256566No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14700.11583258015890338No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14610.11512340109670603No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14600.11504460342312854No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAGAAGGGCACTTTTATAGCTTTGAC14520.11441422203450864No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACAT14180.11173510113287415No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACC14110.11118351741783176No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTACCAATTCTTTAGCCTGGTATCAGCAGAA14060.11078952904994432No Hit
ATAGTAGATAGACCCAATCCACTCCAGTCCCTTCCCTGGAGGCTGCCGGA14030.11055313602921187No Hit
AGTCAGGGCATTACCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAA13980.11015914766132444No Hit
ACTTATTACTGTCAAAGCTATAAAAGTGCCCTTCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA13860.10921357557839462No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13670.1077164197804224No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG13650.10755882443326742No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC13500.10637685932960515No Hit
CATCTATGGTGCATTCACCTTGCAATCCGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGAGTTC13280.10464331051090046No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13120.1033825477336607No Hit
GTATCAGGGAGAACTCCTTCTTGGACAGGTCCACAGACAAGGTGACTCGA12940.10196418960926597No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12900.10164899891495605No Hit
CCATAGATGAGGAGCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGC12820.10101861752633615No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12750.10046703381129377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)