FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250983
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAGACGAACTGTGGCTGCACCAT88720.7092022833243937No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT65080.5202308904277676No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59750.4776243961748481No Hit
AACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGTGAA52840.4223878342071795No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT42770.3418911368100126No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42160.33701497142647024No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG41140.32886138340808785No Hit
TCTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAATTCTTATTGTTGGAGCTGTAAAAAACATT38470.3075181677129106No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA35170.28113891235932065No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG34860.27866086109883187No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33610.2686687189194418No Hit
GTCCTGGTTTGTGCTGGTACCAAGCTAAGTAATTCTTATTGTTGGAGCTGTAAAAAACATTCTGGCTGGACTTGC33560.26826903323226614No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31520.25196185719550146No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC30930.24724556608682935No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA29750.23781298386948502No Hit
CTCCTAAGATGCTCATTTACTGGGCATCTACCCGTGAATCCGGGGTCCCT29480.23565468115873678No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG29250.23381612699772897No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28880.23085845291262952No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC28350.22662178462856808No Hit
GTATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC27610.22070643645836913No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27590.2205465621834989No Hit
GTTTATTACTGTCACCAATATTATACTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAGACGA25980.20767668305644443No Hit
GTCCCTGACCGATTCGTTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC25680.20527856893339078No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATACTACTCCTCGGACGTT25350.20264064339803178No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC24680.19728485518987868No Hit
ATACTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAGACGA24410.1951265524791304No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22960.18353566755103787No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22650.18105761629054912No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22180.17730057083109843No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC21700.17346358823421262No Hit
GTCAGGGACCCCGGATTCACGGGTAGATGCCCAGTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTGTGCTGGT21370.17082566269885363No Hit
GTACCAGCACAAACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGTGAATCCGGGGTCCCTG20840.1665889944147922No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC20830.1665090572773571No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTTTACAGCTCCAACAATAAGAATT20580.16451062884147907No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20280.16211251471842544No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20160.16115326906920396No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20130.1609134576568986No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19730.15771597215949376No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19270.1540388638374782No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT18300.14628496150627146No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATACTACT18290.14620502436883634No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT18240.14580533868166073No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17690.14140879612272908No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17320.1384511220376296No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17280.13813137348788912No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT17000.13589313363970573No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16960.13557338508996525No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGAGTAGT16820.13445426516587355No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC16760.13397464234126283No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG16450.13149659108077408No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA16390.13101696825616335No Hit
GCACAAACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGTGAATCCGGGGTCCCTGACCGATT16300.13029753401924726No Hit
GGACCAAGGTGGAAGTCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA16180.1293382883700258No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC16170.1292583512325907No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC16090.12861885413310972No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15880.12694017424697218No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15880.12694017424697218No Hit
GGTTTGTGCTGGTACCAAGCTAAGTAATTCTTATTGTTGGAGCTGTAAAAAACATTCTGGCTGGACTTGCAGTTG15620.12486180867365904No Hit
TCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATACTACTCCTCGGACGTTCGGCC15450.12350287733726198No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15450.12350287733726198No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15280.12214394600086492No Hit
GAATTACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGTGA15100.12070507752703274No Hit
GTGCTGGTACCAAGCTAAGTAATTCTTATTGTTGGAGCTGTAAAAAACAT15070.12046526611472737No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC15010.11998564329011666No Hit
AGGTGGAAGTCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15010.11998564329011666No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCGGATTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG14820.11846683767884934No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14670.11726778061732254No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG14540.11622859783066597No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14260.11399035798248258No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATACTACTCCTC14060.11239161523378016No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC14040.1122317409589099No Hit
TTATACTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAGACGAACTGTGGCTGCACCATCTGTCTT13970.11167218099686407No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13940.11143236958455871No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCGGATTCCCTGGCTGTGTCTCTGGGCGAGAGGG13930.11135243244712358No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13910.11119255817225333No Hit
GTGCAGCCACAGTTCGTCTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGAGTAGTATAATATTGGTGAC13850.11071293534764262No Hit
GTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAATTCTTATTGTTGGAGCTGTA13810.11039318679790214No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC13720.10967375256098605No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13530.10815494694971875No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG13510.10799507267484851No Hit
GTTTTTTACAGCTCCAACAATAAGAATTACTTAGCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAGATGCTC13420.10727563843793242No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13370.10687595275075681No Hit
GCTTGGTACCAGCACAAACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGTGAATCCGGGGTC13360.10679601561332168No Hit
GTCTCCGGATTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTTT13220.10567689568922999No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC13110.10479758717744365No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGG13080.1045577757651383No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12890.10303897015387099No Hit
GGTCTGGGACAGATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAAT12860.10279915874156563No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATG12860.10279915874156563No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12830.10255934732926028No Hit
CATTTACTGGGCATCTACCCGTGAATCCGGGGTCCCTGACCGATTCGTTGGCAGCGGGTCTGGGACAGATTTC12600.1007207931682525No Hit
TTATTACTGTCACCAATATTATACTACTCCTCGGACGTTCGGCCAAGGGA12520.10008129606877152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)