FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250983
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAGACGAACTGTGGCTGCACCAT83540.667794846133001No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61250.48961496679011624No Hit
GTATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC57060.4561213062048005No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT54490.4355774618839745No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42940.3432500681464097No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA38750.30975640756109396No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT37950.3033614365662843No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG37400.2989648940073526No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34980.2796201067480533No Hit
TCTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAATTCTTATTGTTGGAGCTGTAAAAAACATT34310.2742643185399002No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33260.2658709191092125No Hit
GTCCTGGTTTGTGCTGGTACCAAGCTAAGTAATTCTTATTGTTGGAGCTGTAAAAAACATTCTGGCTGGACTTGC29280.23405593841003433No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC28620.22878008733931637No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG28610.22870015020188122No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28320.22638197321626272No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28280.22606222466652223No Hit
AACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGTGAA27050.21622995676200238No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGA26990.21575033393739165No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC26160.2091155515302766No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC26050.20823624301849025No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25740.20575819175800153No Hit
GTTTATTACTGTCACCAATATTATACTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAGACGA25310.20232089484829133No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG25280.20208108343598594No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA24480.19568611244117623No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24430.19528642675400065No Hit
GTCCCAGACCCGCTGCCAACGAATCGGTCAGGGACCCCGGATTCACGGGTAGATGCCCAGTAAATGAGCATCTT23510.18793221010996952No Hit
ATACTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAGACGA23300.18625353022383198No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23270.18601371881152662No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATACTACTCCTCGGACGTT23160.1851344102997403No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC22690.1813773648402896No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22570.18041811919106815No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22280.17809994220544964No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21760.17394321105882335No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGAGTAGT20560.16435075456660883No Hit
GTCAGGGACCCCGGATTCACGGGTAGATGCCCAGTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTGTGCTGGT19830.158515343533845No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT19550.1562771036856616No Hit
GAGTAGTATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT19530.15611722941079137No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC19400.15507804662413477No Hit
GTGCAGCCACAGTTCGTCTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGAGTAGTATAATATTGGTGAC18370.14684452146831733No Hit
GTCCCTGACCGATTCGTTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC18210.1455655272693554No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAACGAATCGGTCAGGGACC18040.14420659593295831No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17750.1418884189473398No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCAACGAATCGGTCAGGGACCCCG17380.13893074486224033No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17330.13853105917506472No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17210.1375718135258433No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17180.1373320021135379No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATACTACT16940.135413510815095No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT16790.1342144537535682No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16420.13125677966846871No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC16370.1308570939812931No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16320.1304574082941175No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC16170.1292583512325907No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC16060.12837904272080436No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA16050.12829910558336924No Hit
CTCCTAAGATGCTCATTTACTGGGCATCTACCCGTGAATCCGGGGTCCCT15900.12710004852184242No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15730.12574111718544537No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15730.12574111718544537No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC15660.12518155722339952No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG15390.12302325451265124No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15120.12086495180190299No Hit
AGGTGGAAGTCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG14790.118227026266544No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14770.11806715199167374No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG14420.11526935218144452No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14290.11423016939478794No Hit
TCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATACTACTCCTCGGACGTTCGGCC14230.1137505465701772No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATACTACTCCTC14230.1137505465701772No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT14200.11351073515787184No Hit
GGACCAAGGTGGAAGTCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA14170.11327092374556649No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA13890.11103268389738309No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTTTACAGCTCCAACAATAAGAATT13860.11079287248507773No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC13720.10967375256098605No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13650.1091141925989402No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13640.10903425546150507No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAACGAATCGGTC13640.10903425546150507No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13540.10823488408715386No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATG13520.10807500981228362No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC13490.10783519839997827No Hit
TTATACTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAGACGAACTGTGGCTGCACCATCTGTCTT13420.10727563843793242No Hit
GTGCTGGTACCAAGCTAAGTAATTCTTATTGTTGGAGCTGTAAAAAACAT13260.10599664423897048No Hit
GGTTTGTGCTGGTACCAAGCTAAGTAATTCTTATTGTTGGAGCTGTAAAAAACATTCTGGCTGGACTTGCAGTTG13210.10559695855179488No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13180.1053571471394895No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGG13050.10431796435283293No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12960.10359853011591685No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12910.10319884442874123No Hit
GGTCTGGGACAGATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAAT12850.10271922160413052No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12700.1015201645426037No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12520.10008129606877152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)