FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004717631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767115
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT60020.33964965494605615No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCGTAGTCCC57120.3232387252668898No Hit
GATTATTACTGCTGCTCATATGCAGGCAGTTTCACTTCCGTCTTCGGATCTGGGACTACGGTCACCGTCCTACGT52040.2944913036220053No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC48230.272930737388342No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG47680.2698183196905691No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC43700.24729573344123046No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG43410.24565464047331384No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCTGCTCATAT41090.23252589672998078No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG41000.23201659201579974No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGCTATAACTATGTCTCCTGGT39440.22318864363666202No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC38320.2168506294157426No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC35670.2018544350537458No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG34200.19353579138878907No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCGTAGTCCCAGATCCGAAGACGGAAGTGAA33740.19093267840519718No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33440.18923499602459376No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGCTATAACTATGTCTCCTGGTATCAGCATCACCCAGGCAAAGC33250.18815979718354495No Hit
GACGTAGGACGGTGACCGTAGTCCCAGATCCGAAGACGGAAGTGAAACTGCCTGCATATGAGCAGCAGTAATAAT32980.18663188304100187No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC32710.1851039688984588No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC31940.1807465841215767No Hit
ACGTAGGACGGTGACCGTAGTCCCAGATCCGAAGACGGAAGTGAAACTGCCTGCATATGAGCAGCAGTAATAATC31560.17859618643947903No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT31170.1763891993446946No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG31050.17571012639245326No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT31000.17542717932901933No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG29350.16608992623570057No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28730.16258138264912017No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGCTATAACTATGTCTCCTGGTATCAGCATC27680.15663949431700822No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG26710.15115032128639053No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC26530.15013171185802848No Hit
GTCAATAACCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCAT25820.14611386355726708No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25630.14503866471621824No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC24650.13949290227291378No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT24240.13717273635275576No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG24180.1368331998766351No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA24160.13672002105126152No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG24030.13598435868633338No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC23420.13253240451243975No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG23070.13055177506840243No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG23010.1302122385922818No Hit
GTCCAGGAGACCCGGACACTGAGCGAGGCTGAGTCAGGGCAGACTGAGCCCAGGATCCTGTGCCCTGAGTGAGG22970.12998588094153465No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGAC22830.12919362916391972No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG22590.127835483259437No Hit
GCCTTGGGCTGACGTAGGACGGTGACCGTAGTCCCAGATCCGAAGACGGAAGTGAAACTGCCTGCATATGAGC22400.12676028441838816No Hit
CCCCTGAGGGCCGGTTATTGACATCATAAATGATGACTTTGGGGGCTTTGCCTGGGTGATGCTGATACCAGG22120.1251757808631583No Hit
TAGTTATAGCCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGAC21640.12245948905419285No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT21360.120874985498963No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC21240.12019591254672163No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGG21180.11985637607060096No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG20970.11866799840417858No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20780.11759279956312972No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT20430.1156121701190924No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG20220.11442379245267002No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGGTTATTG19590.11085865945340287No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACACTGAGCGAGG19440.11000981826310115No Hit
CTTTGGGGGCTTTGCCTGGGTGATGCTGATACCAGGAGACATAGTTATAG19390.10972687119966726No Hit
GACCATCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGTTTCACTTCCGT19160.1084253147078713No Hit
GGCTATAACTATGTCTCCTGGTATCAGCATCACCCAGGCAAAGCCCCCAAAGTCATCATTTATGATGTCAATAAC19150.10836872529518453No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18280.10344544639143463No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC18200.10299273108994038No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC18140.1026531946138197No Hit
GTTATTGACATCATAAATGATGACTTTGGGGGCTTTGCCTGGGTGATGCTGATACCAGGAGACATAGTTATAGCC18080.10231365813769902No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17750.10044620751903527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)