FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004717632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767115
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT54710.30960067680937575No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCGTAGTCCC53920.3051301132071201No Hit
GATTATTACTGCTGCTCATATGCAGGCAGTTTCACTTCCGTCTTCGGATCTGGGACTACGGTCACCGTCCTACGT50250.28436179875107165No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC48700.27559043978462067No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46490.26308417958084224No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG45420.25702911242335674No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC43590.2466732499016759No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG42990.24327788514046908No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG40070.22675377663592922No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCTGCTCATAT38680.2188878482724667No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGCTATAACTATGTCTCCTGGT38380.21719016589186327No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC35410.2003831103238895No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGCTATAACTATGTCTCCTGGTATCAGCATCACCCAGGCAAAGC32280.18267062415292723No Hit
GACGTAGGACGGTGACCGTAGTCCCAGATCCGAAGACGGAAGTGAAACTGCCTGCATATGAGCAGCAGTAATAAT31870.18035045823276924No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC31860.18029386882008244No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC31810.18001092175664854No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCGTAGTCCCAGATCCGAAGACGGAAGTGAA31260.1768985040588756No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT30970.175257411090959No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG30010.16982482747302807No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC29780.16852327098123213No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC29330.165976747410327No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC27810.15737515668193638No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG27670.15658290490432145No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG27280.154375917809537No Hit
ACGTAGGACGGTGACCGTAGTCCCAGATCCGAAGACGGAAGTGAAACTGCCTGCATATGAGCAGCAGTAATAATC26850.15194257306400547No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26820.15177280482594513No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGCTATAACTATGTCTCCTGGTATCAGCATC26630.1506976059848963No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26480.14984876479459458No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG25350.14345416116098839No Hit
GTCAATAACCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCAT23950.13553164338483914No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC23570.13338124570274149No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG23350.1321362786236323No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC23180.13117425860795703No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA22950.1298727021161611No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22740.1286843244497387No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG22560.12766571502137666No Hit
TAGTTATAGCCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGAC21490.12161064786389116No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG21450.12138429021314402No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT21370.12093157491164978No Hit
GCCTTGGGCTGACGTAGGACGGTGACCGTAGTCCCAGATCCGAAGACGGAAGTGAAACTGCCTGCATATGAGC21140.11963001841985384No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21080.11929048194373314No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGAC21080.11929048194373314No Hit
CCCCTGAGGGCCGGTTATTGACATCATAAATGATGACTTTGGGGGCTTTGCCTGGGTGATGCTGATACCAGG20900.11827187251537111No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG20650.1168571371982016No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGG20390.1153858124683453No Hit
GTCCAGGAGACCCGGACACTGAGCGAGGCTGAGTCAGGGCAGACTGAGCCCAGGATCCTGTGCCCTGAGTGAGG20320.11498968657953784No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT20020.11329200419893443No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT19730.1116509112310178No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG19400.10978346061235403No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19320.10933074531085979No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC19120.10819895705712418No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG19060.1078594205810035No Hit
GGGTGATGCTGATACCAGGAGACATAGTTATAGCCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTG19040.10774624175562994No Hit
GACCATCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGTTTCACTTCCGT18680.10570902289890585No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18620.10536948642278515No Hit
GGCTATAACTATGTCTCCTGGTATCAGCATCACCCAGGCAAAGCCCCCAAAGTCATCATTTATGATGTCAATAAC18560.1050299499466645No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT18090.10237024755038579No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACACTGAGCGAGG18060.10220047931232545No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGGTTATTG17940.1015214063600841No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17890.1012384592966502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)