FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805581
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24630.3057420669057488No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT24410.30301111868328573No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCCACC21450.2662674516901466No Hit
CAATAATACACAGCCGTGTCCTCGGCTTTCAGGCTATTCATTTGAAGATA19770.24541293799133793No Hit
ATAATACACAGCCGTGTCCTCGGCTTTCAGGCTATTCATTTGAAGATATAGTGTGTTCTTGGAATTGTCTCTGG17970.22306881617118576No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17350.21537250754424447No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG16480.20457284866450426No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGGT16090.19973162227013794No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15810.19625586998700317No Hit
GTATTATTGTGTGAGAGATAATGTGAATACAGCCATGGTCAGGGGACAATATCTCTACTACCACCTCGGTATGG15500.19240771567353251No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG15100.18744235526905428No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT15060.18694581922860642No Hit
CTCCTGCAGGGTCAGTCAGAGTATTAACACGAACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT14160.17577375831853034No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13440.1668361095904695No Hit
AACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTTCGGTGCATCCACCAGGGCC13060.16211901720621513No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12930.16050527507475973No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12820.1591398009635282No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA12780.15864326492308037No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12280.15243656441748255No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT12120.15045042025569122No Hit
CTCCTGTGTAGCCTCTGGAATCACCGTCAGCATCAATCACATGAGCTGGGTCCGCCAGGCTCCAGGAAAGGGG12000.14896081213434775No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11990.1488366781242358No Hit
GTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAAGAGCCACCCTCTCCT11930.14809187406356406No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA11840.14697466797255646No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAATTTATTACTGTCAACAATATAATGACTGG11750.14585746188154886No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11720.145485059851213No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11630.14436785376020536No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11620.14424371975009342No Hit
GTATTAACACGAACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTTCGGTGCATCC11540.14325064766919776No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC11510.1428782456388619No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG11480.14250584360852603No Hit
ATATAATGACTGGCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAGACGAACTGTGGCTGCACCAT11440.1420093075680782No Hit
GTGTATTATTGTGTGAGAGATAATGTGAATACAGCCATGGTCAGGGGACA11350.1408921014770706No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC11220.13927835934561514No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGGGGAGGCCAGTCATTATATTGTTGACAGTAATAAATTGCAAAATCTTC11000.1365474111231521No Hit
GTACGCAGACTCCGTGAGGGGCAGATTCACCATCTCCAGAGACAATTCCA10200.1266166903141956No Hit
GAACACAGAGGACTCGCCATGGATTTTGGGCTGAGCTGGGTTTTCCTCGT10200.1266166903141956No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGGTGGATGCACCGA10090.12525121620296406No Hit
GTCATTATATTGTTGACAGTAATAAATTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT9970.1237616080816206No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGATTTTGGGCTGAGCTGGG9930.12326507204117278No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9820.12189959792994125No Hit
CCACTGGACAAGTAGTAGTGACGCAGTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAAGAGCCACCCTCTC9680.12016172178837385No Hit
CTTAAAAGGTGTCCAGTGTGAGGAGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTG9620.1194169177277021No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCTACCTTGGTCCCTCCGCCGAAAGTGAGGGGAGGCC9510.1180514436164706No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9450.11730663955579887No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9440.11718250554568689No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGC9420.116934237525463No Hit
GCACTGAACACAGAGGACTCGCCATGGATTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTATCTTAAAAGGTGTCC9140.1134584852423282No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGCACCCAGCACTGGGATTCCGAGGTGTTTCCATTCAGTGAT8900.11047926899964125No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8850.10985859894908145No Hit
CACTATATCTTCAAATGAATAGCCTGAAAGCCGAGGACACGGCTGTGTATTATTGTGTGAGAGATAATGTGAAT8840.10973446493896952No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8790.10911379488840972No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGAGGTGGT8780.10898966087829777No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC8750.1086172588479619No Hit
ACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTTCGGTGCATCCACCAGGGCCACTGGCATCCCAGCCAGGTT8640.10725178473673037No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8630.10712765072661842No Hit
GAGTATTAACACGAACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTTCGGTGCATC8530.10588631062549887No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8490.10538977458505103No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8490.10538977458505103No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8470.10514150656482713No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8430.1046449705243793No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8400.10427256849404343No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG8390.10414843448393148No Hit
AGGTAGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG8330.10340363042325973No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCCACCACTGGACAAGT8300.10303122839292386No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8270.102658826362588No Hit
GCACTGGGATTCCGAGGTGTTTCCATTCAGTGATCTGCACTGAACACAGAGGACTCGCCATGGATTTTGGGCTG8200.10178988829180431No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC8180.10154162027158038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)