FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805581
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25270.313686643552914No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT20830.25857114306320533No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCCACC20640.25621259687107817No Hit
CAATAATACACAGCCGTGTCCTCGGCTTTCAGGCTATTCATTTGAAGATA16980.21077954917010205No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA15440.19166291161286078No Hit
ATAATACACAGCCGTGTCCTCGGCTTTCAGGCTATTCATTTGAAGATATAGTGTGTTCTTGGAATTGTCTCTGG15130.18781475729939015No Hit
CTCCTGCAGGGTCAGTCAGAGTATTAACACGAACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT15100.18744235526905428No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15030.18657341719827056No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGGT14900.18495967506681513No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT14230.17664269638931404No Hit
GTATTATTGTGTGAGAGATAATGTGAATACAGCCATGGTCAGGGGACAATATCTCTACTACCACCTCGGTATGG14130.17540135628819448No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13850.1719256040050597No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13320.16534650146912602No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13150.16323622329722276No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG12540.1556640486803934No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT12540.1556640486803934No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12000.14896081213434775No Hit
CTCCTGTGTAGCCTCTGGAATCACCGTCAGCATCAATCACATGAGCTGGGTCCGCCAGGCTCCAGGAAAGGGG11820.14672639995233253No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11800.14647813193210862No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11760.1459815958916608No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC11540.14325064766919776No Hit
ATATAATGACTGGCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAGACGAACTGTGGCTGCACCAT11430.14188517355796623No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAATTTATTACTGTCAACAATATAATGACTGG10940.13580260706248037No Hit
AACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTTCGGTGCATCCACCAGGGCC10890.13518193701192058No Hit
GTATTAACACGAACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTTCGGTGCATCC10800.13406473092091298No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10530.13071311264789015No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG10400.12909937051643472No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC10360.1286028344759869No Hit
GTACGCAGACTCCGTGAGGGGCAGATTCACCATCTCCAGAGACAATTCCA10310.12798216442542712No Hit
GTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAAGAGCCACCCTCTCCT10280.12760976239509125No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10280.12760976239509125No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10220.12686495833441952No Hit
GTGTATTATTGTGTGAGAGATAATGTGAATACAGCCATGGTCAGGGGACA10210.12674082432430755No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGGGGAGGCCAGTCATTATATTGTTGACAGTAATAAATTGCAAAATCTTC10180.1263684222939717No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGGTGGATGCACCGA10040.12463054615240428No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC9990.12400987610184451No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9760.1211547938692695No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9400.11668596950523907No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9390.11656183549512712No Hit
GTCATTATATTGTTGACAGTAATAAATTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT9210.11432742331311191No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCTACCTTGGTCCCTCCGCCGAAAGTGAGGGGAGGCC9120.11321021722210431No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGC9080.11271368118165646No Hit
GAACACAGAGGACTCGCCATGGATTTTGGGCTGAGCTGGGTTTTCCTCGT8930.11085167102997712No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG8900.11047926899964125No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC8820.10948619691874559No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGAGGTGGT8750.1086172588479619No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8510.10563804260527496No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8490.10538977458505103No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA8420.10452083651426734No Hit
CTTAAAAGGTGTCCAGTGTGAGGAGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTG8260.10253469235247605No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8210.10191402230191626No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8180.10154162027158038No Hit
CCACTGGACAAGTAGTAGTGACGCAGTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAAGAGCCACCCTCTC8160.10129335225135647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)