FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652122
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCAGTTTCAGGGAGA28650.4393349710636966No Hit
ATATTACTGTCAGCAGTATGGTAGCTCACCAAAGACGTTCGGCCAAGGGA20230.3102180266882577No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19520.29933049337393924No Hit
GTCTCTGGTGGCTCCATCAGTAGTTCCTACTGGAGCTGGATCCGGCAGCC19220.2947301271847906No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT19190.2942700905658757No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCC17100.26222087278147344No Hit
GTATATTACTGTCAGCAGTATGGTAGCTCACCAAAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA15560.23860565967717698No Hit
GTGTATTACTGTGCGAGAGATGTGGCATATAGCAGTGGTTGGGGCTACTT14920.2287915451403265No Hit
GTATGGTAGCTCACCAAAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT14490.22219768693588013No Hit
GTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCCGATGGTGAGAGTGAAGTCTGTCCCAGACGCACTGCC13880.21284360901794452No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13690.209930043764817No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13480.20670978743241294No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13240.20302949448109403No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12510.19183527008749895No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12380.1898417780722012No Hit
ATCTATAACAATGGGGACACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGCAGACACGTCCA12140.1861614851208823No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG11930.18294122878847824No Hit
GGGTATATCTATAACAATGGGGACACCAACTACAACCCCTCCCTCAAGAG11920.18278788324883993No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11880.1821745010902868No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTCCTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGG11830.18140777339209535No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCCGATGGTGAGAGTGAAGTCTGTCCCAGACGCACTGCC11770.1804877001542656No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11480.17604067950475524No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCAGTTTCAGGGAGAACTGGTTCTTGGACGTGTCTG11460.17573398842547866No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG10910.1672999837453728No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTGAGC10510.16116616215984125No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10350.15871263352562864No Hit
CTCCTGCAGGGCCAGTCAGAGTGTGAGCAGCAACTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCC9990.15319219409865026No Hit
GTCAGAGTGTGAGCAGCAACTATTTAGCCTGGTACCAGCAGAAACCTGGC9430.1446048438789061No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT9210.14123124200686374No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCGGCAGGGCCACTGGCATCCCA9160.1404645143086723No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTGAGCAGCA9130.14000447768975743No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9020.13831767675373627No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA9000.13801098567445969No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA8950.13724425797626824No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8900.1364775302780768No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG8890.13632418473843852No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8680.13310392840603444No Hit
GTATTACTGTGCGAGAGATGTGGCATATAGCAGTGGTTGGGGCTACTTTGACTACTGGGGCCAGGGAACCCTGGT8550.13111043639073672No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTAGCCCCAAC8530.13080374531146013No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8450.12957698099435383No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG8440.12942363545471552No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8410.12896359883580066No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8370.12835021667724753No Hit
CTACCATACTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCCGATGGTGAGAGTGAAGTCTGTC8350.12804352559797094No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC8320.12758348897905608No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC8220.12605003358267317No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC8140.12482326926556686No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCC8070.12374985048809885No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7980.12236974063135427No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7970.12221639509171597No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7930.12160301293316282No Hit
GTCTGGGACAGACTTCACTCTCACCATCGGCAGACTGGAGCCTGAAGATT7600.11654261012509928No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTC7570.11608257350618442No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7560.11592922796654613No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA7540.11562253688726955No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7430.1139357359512484No Hit
CATCTATGGTGCATCCGGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGCGTCTGGGACAGACTTC7410.11362904487197181No Hit
GTTATAGATATACCCAATCCACTGCAGTCCCTTCCCTGGGGGCTGCCGGA7400.11347569933233352No Hit
CTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGCAGACACGTCCA7380.11316900825305695No Hit
CTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGGTGCATCCGGCAGGGCC7360.11286231717378037No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGACTCCTGCAGCTGCAC7330.11240228055486551No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT7330.11240228055486551No Hit
GGATTGGGTATATCTATAACAATGGGGACACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGC7310.11209558947558892No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCTTTGGTGAGCTACCATACTGCTGAC7290.11178889839631234No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7270.11148220731703577No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7200.11040878853956775No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7180.11010209746029118No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC7120.10918202422246144No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7030.10780191436571683No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAATAGTTGCTGCTCACACTCTGACTGGCCCTGCAGG7010.10749522328644026No Hit
GACTTCACTCTCACCATCGGCAGACTGGAGCCTGAAGATTTTGCAGTATATTACTGTCAGCAGTATGGTAGCTC6980.10703518666752541No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCTTTGGTGAGCT6960.10672849558824883No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC6950.10657515004861054No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6810.1044283124936745No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC6760.10366158479548306No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG6660.10212812939910017No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG6630.10166809278018529No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGCCCCAACCACTGCTATATGCCACATCTCTCGCACAGTAATACACGG6620.10151474724054702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)