FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652122
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCAGTTTCAGGGAGA26000.3986984030595502No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20990.32187228770076765No Hit
ATATTACTGTCAGCAGTATGGTAGCTCACCAAAGACGTTCGGCCAAGGGA18460.28307586617228064No Hit
GTCTCTGGTGGCTCCATCAGTAGTTCCTACTGGAGCTGGATCCGGCAGCC17420.26712793004989865No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT15580.23891235075645356No Hit
GTGTATTACTGTGCGAGAGATGTGGCATATAGCAGTGGTTGGGGCTACTT15260.2340052934880283No Hit
GTATATTACTGTCAGCAGTATGGTAGCTCACCAAAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA14680.22511125218900757No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCC14530.22281106909443324No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13500.20701647851168953No Hit
GTATGGTAGCTCACCAAAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT13340.20456294987747692No Hit
GTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCCGATGGTGAGAGTGAAGTCTGTCCCAGACGCACTGCC12540.19229530670641384No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12150.1863148306605206No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCCGATGGTGAGAGTGAAGTCTGTCCCAGACGCACTGCC11560.17726744382186155No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11220.17205369547415975No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTCCTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGG11210.17190034993452144No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11130.17067358561741516No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA10790.16545983726971333No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCAGTTTCAGGGAGAACTGGTTCTTGGACGTGTCTG10590.16239292647694759No Hit
ATCTATAACAATGGGGACACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGCAGACACGTCCA10570.162086235397671No Hit
CTCCTGCAGGGCCAGTCAGAGTGTGAGCAGCAACTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCC10290.1577925602877989No Hit
GGGTATATCTATAACAATGGGGACACCAACTACAACCCCTCCCTCAAGAG10090.15472564949503315No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9970.15288550301937368No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9860.1511987020833525No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9850.1510453565437142No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9650.14797844575094843No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9440.14475818941854438No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT9270.1421513152446935No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9200.14107789646722546No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTGAGC9040.13862436783301285No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG9030.13847102229337457No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCGGCAGGGCCACTGGCATCCCA8810.13509742042133221No Hit
GTATTACTGTGCGAGAGATGTGGCATATAGCAGTGGTTGGGGCTACTTTGACTACTGGGGCCAGGGAACCCTGGT8520.13065039977182186No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTGAGCAGCA8420.12911694437543897No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8190.1255899969637583No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8150.12497661480520515No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA8140.12482326926556686No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG8110.124363232646652No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTAGCCCCAAC8030.12313646832954571No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC7940.12175635847280111No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG7930.12160301293316282No Hit
CTACCATACTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCCGATGGTGAGAGTGAAGTCTGTC7900.12114297631424795No Hit
GTCAGAGTGTGAGCAGCAACTATTTAGCCTGGTACCAGCAGAAACCTGGC7870.12068293969533309No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7680.1177693744422056No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7660.11746268336292902No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCC7650.11730933782329073No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC7630.11700264674401414No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7580.1162359190458227No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCTTTGGTGAGCTACCATACTGCTGAC7560.11592922796654613No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA7400.11347569933233352No Hit
GTCTGGGACAGACTTCACTCTCACCATCGGCAGACTGGAGCCTGAAGATT7380.11316900825305695No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7260.11132886177739748No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7130.10933536976209972No Hit
CTGCTATATGCCACATCTCTCGCACAGTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCAGTTTCAGGG7090.10872198760354657No Hit
CTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGCAGACACGTCCA7010.10749522328644026No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG7010.10749522328644026No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGCCCCAACCACTGCTATATGCCACATCTCTCGCACAGTAATACACGG6990.1071885322071637No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAATAGTTGCTGCTCACACTCTGACTGGCCCTGCAGG6970.10688184112788712No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC6920.10611511342969568No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC6840.10488834911258936No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC6830.10473500357295108No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC6810.1044283124936745No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6780.10396827587475964No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT6690.10258816601801503No Hit
GTCCCAGACGCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCC6660.10212812939910017No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6660.10212812939910017No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTC6660.10212812939910017No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG6660.10212812939910017No Hit
CATCTATGGTGCATCCGGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGCGTCTGGGACAGACTTC6620.10151474724054702No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG6580.10090136508199385No Hit
GACTTCACTCTCACCATCGGCAGACTGGAGCCTGAAGATTTTGCAGTATATTACTGTCAGCAGTATGGTAGCTC6540.1002879829234407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)