FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886105
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAACTGGCCTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT40060.45209089216289267No Hit
GTTATAATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT37930.42805310883021763No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33050.37298062870653026No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTCTGG25560.2884533999921003No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT24700.2787480039047291No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC22960.259111504844234No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20070.22649685985295198No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT18920.21351871392216495No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17880.20178195586301848No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA17300.1952364561761868No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16860.19027090468962482No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16780.18936807714661355No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16490.1860953273031977No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT16030.1809040689308829No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCT14940.16860304365735437No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTC14900.16815162988584875No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG14850.1675873626714667No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTG14210.16036474232737655No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATTATAACTGG13950.15743055281258994No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT13890.15675343215533147No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG13450.1517878806687695No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGT13260.14964366525411774No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13120.14806371705384802No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC12630.142533898352904No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12610.14230819146715118No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12070.13621410555182514No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11990.13531127800881385No Hit
GTGTATTACTGTGCGAGAGATACAGACAAAATCTATTACTATGATAGTAGGGGTTATAACTTTGGTGCTTTTGAT11730.13237708849402724No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC11660.13158711439389237No Hit
CCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG11630.13124855406526315No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC11460.1293300455363642No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC11460.1293300455363642No Hit
GTTTATTACTGTCAGCAGTATTATAACTGGCCTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA11370.1283143645504765No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11280.12729868356458884No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCC11220.1266215629073304No Hit
CCATAGATGAGGAGCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGGC11200.12639585602157757No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11120.1254930284785663No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG11040.12459020093555503No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC10790.12176886486364484No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA10640.12007606322049871No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGCTACTAAAGGTGATTCCAGAGGCTGCAC10590.11951179600611665No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAAGGAGGCCAGTTATAATACTG10520.1187218219059818No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10480.11827040813447616No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC10350.11680331337708286No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAAGGAGGCC10320.11646475304845363No Hit
GGAGAAAGAGTGATGACGCAATCTCCAGCCACCCTGTCTGTGTCTCCAGG10250.11567477894831876No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA9890.11161205500476805No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG9520.10743647761834094No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC9450.10664650351820608No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAAGAGTGATGACGCAATCTCCAGCCACCCTGTCTGTGTCTCC9400.10608223630382405No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA9150.10326090023191382No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGATGCACCAT9150.10326090023191382No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT9030.10190665891739693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)