FastQCFastQC Report
Wed 8 May 2024
EGAF00004717650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443487
Total Bases106.1 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT44580.30883547964062025No Hit
GTAATAAAAGGCCGTGTCCTCGTCTCCCAGGCTGTTCATTTGCAGATAGA41760.28929945333764695No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT40170.2782844597838429No Hit
GTCTATGTCTCCAGGTGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGGA38900.26948632027860314No Hit
GTGCTACGCTGCTGACAAAAATAAACTGCAAAATCTTCAGGCTCTAGGCT38500.2667152527178977No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38480.26657669933986244No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC36890.25556170578605836No Hit
GCGTAGCACCTGGCCAAGGGGGACGTTCGGCCAAGGGACCAAGGTGGAAA33270.23048354436167423No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31940.22126974472232863No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29080.20145661166328482No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28350.19639941336499742No Hit
GTATGTGGCTCCACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCT28140.19494460289562704No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27840.19286630222509796No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT27420.1899566812863573No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT26970.18683923028056368No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA26900.18635429345744023No Hit
GTCTCCAGCCACCCTGTCTATGTCTCCAGGTGAAAGAGCCACCCTCTCCT25710.17811036746434156No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT25680.17790253739728865No Hit
GTACCAGGTGCTACTATCTTTCGCACAGTAATAAAAGGCCGTGTCCTCGT25560.17707121712907703No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25500.1766555569949712No Hit
CTCCTGCAGGGCCAGTCGGACTGTTGCCAGCTACTTAGCCTGGTACCAAC24920.17263750903194833No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24850.17215257220882488No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24740.1713905286296309No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG24570.17021282491633108No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG24000.16626405364232585No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23930.1657791168192024No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT22450.1555261668445923No Hit
CTTTTATTACTGTGCGAAAGATAGTAGCACCTGGTACAGTGCTTTTGATG22240.15407135637522193No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22090.1530322060399574No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG22060.1528243759729045No Hit
GCCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA21790.15095390536942835No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCAAGGCATAGCGAC21780.1508846286804107No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTTTTGTCAGCAGCGTAGCACC21780.1508846286804107No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT21480.14880632800988164No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC21070.14596598376015857No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA21010.14555032362605275No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA20790.14402623646766474No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT20770.1438876830896295No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20750.14374912971159423No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC20710.1434720229555237No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA20520.1421557658641886No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19990.13848410134625389No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC19960.138276271279201No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19870.13765278107804227No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC19770.1369600141878659No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19580.13564375709653081No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC19420.13453533007224866No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT19390.13432750000519575No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC19190.13294196622484306No Hit
GGGCTGGAGTGGGTCTCAGCTATTAGTGGTAGTGGTGGAGCCACATACTA19140.13259558277975486No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG18970.13141787906645505No Hit
CCTTGGGGCTGGTCGGGGATGCCGAAGAGACGGTGACCATTGTCCCTTGG18890.13086366555431397No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18730.1297552385300318No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC18400.1274691077924498No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG18380.12733055441441454No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCA18350.12712272434736163No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT18310.1268456175912911No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18230.12629140407915002No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17770.12310467638433877No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT17740.12289684631728585No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17490.12116492909184495No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG17340.12012577875658043No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA17090.11839386153113952No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA16960.11749326457391027No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16810.11645411423864573No Hit
CTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCA16790.11631556086061044No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTC16750.1160384541045399No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC16660.11541496390338118No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA16550.1146529203241872No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCCGA16450.11396015343401085No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG16360.11333666323285212No Hit
ACCTGGTACAGTGCTTTTGATGTCTGGGGCCAAGGGACAATGGTCACCGT16310.11299027978776394No Hit
GCCTGGGAGACGAGGACACGGCCTTTTATTACTGTGCGAAAGATAGTAGC16140.11181257607446413No Hit
GCCTTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTC16110.11160474600741123No Hit
GTGTGAGGTGCAACTGTTGGAGTCTGGGGGAGGCTTGTTACAGCCTGGGG16090.11146619262937595No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACC15790.10938789195884686No Hit
CCATTGTCCCTTGGCCCCAGACATCAAAAGCACTGTACCAGGTGCTACTA15710.10883367844670577No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG15620.10821018824554707No Hit
CTGCTACTCTGGCTCCCAGATGCCACCGGAGAAATTGTGTTGACACAGTC15220.10543912068484164No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGC15180.1051620139287711No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15060.10433069366055947No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14920.10336082001431257No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA14840.10280660650217148No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT14740.10211383961199513No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA14640.10142107272181876No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT14620.10128251934378349No Hit
CTCCCAGGCTGTTCATTTGCAGATAGAGTGTGTTCTTGGAATTGTCTCTG14540.10072830583164241No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14530.10065902914262476No Hit
GGACAATGGTCACCGTCTCTTCGGCATCCCCGACCAGCCCCAAGGTCTTC14510.1005204757645895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)