FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610865
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT27040.4426509949006736No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA24580.4023802313113372No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC23690.38781072741113015No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22450.36751164332544833No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACTTTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGT22170.3629279791770686No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21430.35081400964206494No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCGGTGATGTTGGGAGTTATAACCTTGTCTCCTGGT21240.3477036661128073No Hit
GATTATTACTGCTGCTCATATGCAGGTAAAGTCTTCGGCACTGGGACCAA20920.34246519280037324No Hit
GTACCAACACCACCCAGGCAAGGCCCCCAAACTCATGATTTATGACGTCA20650.33804523094300704No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC20100.3290416049372611No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19850.32494904766192206No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC19250.3151269102011083No Hit
GTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACATCACCGCTGGTTCCAGTGCAGGAGATGGTGATCG17480.2861516046917077No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC17080.27960351305116515No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACTTTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTC16830.27551095577582607No Hit
GTTATAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAGGCCCCCAAA16630.27223690995555483No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGTCCAGAGATTGTCAGGGAGGCCGTGTTGTCAGACTTGGAGCCAG15890.2601229404205512No Hit
CTCCTGCACTGGAACCAGCGGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAGGC15630.25586668085419856No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG15600.2553755739811579No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG15460.253083741906968No Hit
TCTCTGGACTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGGTAAAGTCTTCGGCACTGGG14990.24538973422933053No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC14840.2429341998641271No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14800.24227939070007284No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT14560.23835053571574735No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACTTTACCTGCAT14550.2381868334247338No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACTTT14550.2381868334247338No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13910.22770988679986578No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG13640.22328992494249958No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13570.22214400890540462No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCGGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACACC13450.2201795814132419No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13040.2134677874816858No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12830.210030039370401No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG11630.19038576444877345No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA11190.1831828636441767No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG10980.17974511553289188No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10950.1792540086598512No Hit
CCCCTGAGGGCCGCTTAGTGACGTCATAAATCATGAGTTTGGGGGCCTTGCCTGGGTGGTGTTGGTACCAGG10950.1792540086598512No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10830.17728958116768845No Hit
ACCCAGGCAAGGCCCCCAAACTCATGATTTATGACGTCACTAAGCGGCCC10620.17385183305640364No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10570.1730333216013358No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC10480.17156000098221375No Hit
GTCCAGAGATTGTCAGGGAGGCCGTGTTGTCAGACTTGGAGCCAGAGAAG10410.1704140849451188No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG10190.16681263454282044No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9820.16075564977531862No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9740.1594460314472101No Hit
GGTTATAACTCCCAACATCACCGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGG9590.15699049708200666No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC9520.15584458104491172No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9430.15437126042578966No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG8910.1458587412930844No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACC8850.144876527547003No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC8460.13849213819747408No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC8340.1365277107053113No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8290.1357091992502435No Hit
GGTAAAGTCTTCGGCACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC8190.13407217634010787No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8160.1335810694670672No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGACAACACGGCCTCCCTGACAATCTCTGGACTCCAGG8110.13276255801199938No Hit
TGCCTGGGTGGTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACATCA8020.13128923739287732No Hit
GGCAAGGCCCCCAAACTCATGATTTATGACGTCACTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCC7900.12932480990071457No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCC7820.12801519157260605No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT7810.1278514892815925No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA7710.12621446637145686No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC7590.12425003887929412No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT7570.12392263429726699No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC7530.12326782513321274No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG7360.12048488618598217No Hit
GCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGTCCAGAGATTGTCAGGGAGGCCGTGTTGTC7310.11966637473091435No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT7310.11966637473091435No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG7280.11917526785787368No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCCCAGGACCCTGTGTCCTGAGTGAGG7230.11835675640280585No Hit
GCTCATATGCAGGTAAAGTCTTCGGCACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCC7150.11704713807469737No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAG7140.11688343578368379No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAA7130.11671973349267023No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC7080.11590122203760242No Hit
GCCTGGAGTCCAGAGATTGTCAGGGAGGCCGTGTTGTCAGACTTGGAGCC7040.11524641287354817No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG6850.11213606934429048No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC6830.11180866476226334No Hit
ACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG6820.11164496247124979No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCC6750.11049904643415484No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCC6730.11017164185212773No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC6610.10820721435996497No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA6550.10722500061388358No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGA6500.10640648915881577No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG6380.10444206166665303No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC6340.10378725250259878No Hit
TCACTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGACAACACGGCCTCCCTGACAATCT6290.10296874104753095No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT6260.10247763417449028No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC6250.10231393188347672No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCC6240.10215022959246314No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC6220.101822825010436No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA6210.10165912271942247No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG6200.1014954204284089No Hit
ACACCACCCAGGCAAGGCCCCCAAACTCATGATTTATGACGTCACTAAGCGGCCCTCAGGGGTTTCTAATCGCTT6150.10067690897334108No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAG6110.10002209980928684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)