FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610865
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT24970.4087646206608661No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC23190.379625612860452No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCGGTGATGTTGGGAGTTATAACCTTGTCTCCTGGT22560.36931236852659755No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21720.35556137608145827No Hit
GATTATTACTGCTGCTCATATGCAGGTAAAGTCTTCGGCACTGGGACCAA21100.34541183403861736No Hit
GTACCAACACCACCCAGGCAAGGCCCCCAAACTCATGATTTATGACGTCA19420.3179098491483388No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACTTTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGT19090.3125076735448913No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC18860.3087425208515793No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG18600.3044862612852267No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC17380.284514581781572No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17050.27911240617812444No Hit
GTTATAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAGGCCCCCAAA16190.2650340091509581No Hit
CTCCTGCACTGGAACCAGCGGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAGGC16000.2619236656217004No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15750.2578311083463613No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG15270.2499733983777103No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACTTTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTC14990.24538973422933053No Hit
TCTCTGGACTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGGTAAAGTCTTCGGCACTGGG14910.24408011590122203No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14870.2434253067371678No Hit
GTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACATCACCGCTGGTTCCAGTGCAGGAGATGGTGATCG14600.23900534487980157No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT14350.23491278760446252No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC14180.23212984865723196No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13970.22869210054594713No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGTCCAGAGATTGTCAGGGAGGCCGTGTTGTCAGACTTGGAGCCAG13810.22607286388973014No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13220.21641442871992994No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13160.21543221497384857No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCGGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACACC12910.21133965769850946No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACTTTACCTGCAT12520.20495526834898056No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACTTT12420.20331824543884494No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12270.20086271107364145No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT12150.19889828358147874No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12000.19644274921627528No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG11630.19038576444877345No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC11430.1871117186285022No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA11280.18465618426329877No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10900.17843549720478338No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG10860.17778068804072913No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACC10730.1756525582575528No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC10500.17188740556424087No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG10440.1709051918181595No Hit
ACCCAGGCAAGGCCCCCAAACTCATGATTTATGACGTCACTAAGCGGCCC10380.16992297807207812No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10310.16877706203498316No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC9900.1620652681034271No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9870.16157416123038643No Hit
CCCCTGAGGGCCGCTTAGTGACGTCATAAATCATGAGTTTGGGGGCCTTGCCTGGGTGGTGTTGGTACCAGG9870.16157416123038643No Hit
GTCCAGAGATTGTCAGGGAGGCCGTGTTGTCAGACTTGGAGCCAGAGAAG9760.15977343602923721No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG9470.1550260695898439No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9110.14913278711335565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8860.14504022983801657No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT8720.1427483977638267No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8560.1401291611076097No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8500.13914694736152833No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8410.13767362674240627No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGACAACACGGCCTCCCTGACAATCTCTGGACTCCAGG8160.1335810694670672No Hit
GGTTATAACTCCCAACATCACCGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGG8120.13292626030301294No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8000.1309618328108502No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCC7970.1304707259378095No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7770.12719668011753824No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG7730.126541870953484No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC7710.12621446637145686No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT7640.12506855033436193No Hit
ACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG7370.12064858847699574No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC7320.11983007702192792No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG7280.11917526785787368No Hit
GCTCATATGCAGGTAAAGTCTTCGGCACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCC7050.11541011516456173No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA6980.11426419912746678No Hit
GGTAAAGTCTTCGGCACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC6960.11393679454543967No Hit
TGCCTGGGTGGTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACATCA6940.11360938996341254No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCC6920.11328198538138542No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAG6890.11279087850834472No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT6880.11262717621733115No Hit
GCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGTCCAGAGATTGTCAGGGAGGCCGTGTTGTC6800.11131755788922265No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT6740.11033534414314128No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCC6680.10935313039705992No Hit
TACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT6660.10902572581503278No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC6650.10886202352401922No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC6630.1085346189419921No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAA6620.10837091665097853No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTAC6550.10722500061388358No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC6450.10558797770374798No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC6440.1054242754127344No Hit
GGCAAGGCCCCCAAACTCATGATTTATGACGTCACTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCC6410.1049331685396937No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA6390.10460576395766659No Hit
GTATCAACGCAGAGTACGGGGGGGTCTCAGGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG6360.10411465708462589No Hit
ACACCACCCAGGCAAGGCCCCCAAACTCATGATTTATGACGTCACTAAGCGGCCCTCAGGGGTTTCTAATCGCTT6320.10345984792057164No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTAC6290.10296874104753095No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGA6190.10133171813739533No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC6150.10067690897334108No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG6150.10067690897334108No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTGCCGA6110.10002209980928684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)