FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885003
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46730.5280208089690092No Hit
CTCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG46290.5230490744099173No Hit
GTGTAGGAGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTG39710.44869904395804305No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT38160.43118497903396935No Hit
GTACACACTCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA33410.37751284458922735No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30250.34180675093756746No Hit
GTGATGGAAGCACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATACGGTTT29030.32802148693281263No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA27440.3100554461397306No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT27320.30869951853270555No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25210.2848577914425149No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGATGGAAGCACCTACTTGA25190.2846318035080107No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG25000.28248491813022103No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24860.2809030025886918No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTGAAATCAGTGCCTGACCCACTGCC22340.2524285228411655No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGG21620.24429295719901514No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG20440.2309596690632687No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19610.22158116978134534No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG19600.22146817581409328No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19520.22056422407607656No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC19400.21920829646905152No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC19210.21706141109126184No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC18200.2056490203988009No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC18060.20406710485727167No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17790.20101626774146528No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17260.1950275874771046No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17250.19491459350985252No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCGTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAA17050.19265471416481073No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA17030.19242872623030655No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC16740.189151901179996No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16500.18644004596594588No Hit
GTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAGAATC16490.1863270519986938No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCC16490.1863270519986938No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAG16420.18553609422792916No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATACGGTTTCTAACCGGGAC16020.18101633553784563No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGGAGTGTGT15320.17310675783019944No Hit
CTCGTACACAGTGATGGAAGCACCTACTTGAATTGGTTTCAGCAGAGGCC15320.17310675783019944No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGATGG15320.17310675783019944No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15290.17276777592844317No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT15220.17197681815767857No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGGAGTGTGTACCTTGCATGCAGT15110.17073388451790558No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTAC14780.16700508359858668No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATG14240.1609034093669739No Hit
GTGTGAAATCAGTGCCTGACCCACTGCCACTGAATCTGTCTGGGACCCCA14010.15830454812017586No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC13830.15627065670963827No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC13650.1542367652991007No Hit
GATTTCACACTGAGAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTACACACTC13430.15175089801955474No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC13430.15175089801955474No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT13420.15163790405230265No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13280.15005598851077342No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13270.14994299454352134No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC12870.1454232358534378No Hit
AGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACC12790.1445192841154211No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12480.14101647113060634No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT12470.14090347716335425No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC12460.14079048319610216No Hit
GTTTATTACTGCATGCAAGGTACACACTCCTACACTTTTGGCCAGGGGAC12190.13773964608029576No Hit
CAGTGATGGAAGCACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAAT12090.1366097064077749No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12020.13581874863701027No Hit
TTATTACTGCATGCAAGGTACACACTCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG11690.13208994771769136No Hit
GTTAGAAACCGTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGCTTCC11560.1306210261434142No Hit
GTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGG11510.13005605630715375No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11500.12994306233990166No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC11360.12836114679837243No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT11300.1276831829948599No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT11230.12689222522409527No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT11230.12689222522409527No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11140.1258752795188265No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC11090.12531030968256607No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG11090.12531030968256607No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11010.12440635794454935No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC10820.12225947256675966No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10770.12169450273049921No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC10570.11943462338545746No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10500.11864366561469283No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA10490.11853067164744074No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG10420.11773971387667613No Hit
GGTCTAGTCAAAGCCTCGTACACAGTGATGGAAGCACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC10380.11728773800766777No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGATGGAAGCACCTACTTG10260.11593181040064271No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTGAAATCAGTGCCTGACCCACTGCC10090.1140109129573572No Hit
CTCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC10000.11299396725208842No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTACACACTCCTACACTTTTGGCCAGGGGACCA9970.11265498535033215No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9960.11254199138308006No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9960.11254199138308006No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9850.11129905774330709No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTC9840.111186063776055No Hit
AATTTATACGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGTGGCAGTGGGTCAGGCACTGATTTCAC9760.11028211203803828No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGCTTCCATCACTGTGTACGAGGCTTTGACTAGACCTGCAGG9740.11005612410353412No Hit
CCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAG9690.10949115426727367No Hit
AGTCAAAGCCTCGTACACAGTGATGGAAGCACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGG9610.10858720252925695No Hit
GAGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTGAAATC9500.10734426888948398No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC9410.1063273231842152No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9380.10598834128245893No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9330.1054233714461985No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9280.10485840160993805No Hit
GGCCAAAAGTGTAGGAGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATT9080.10259852226489627No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT9080.10259852226489627No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9000.10169457052687957No Hit
GCCTCCACCCTGCTGATTCTCAGTGTGAAATCAGTGCCTGACCCACTGCC8970.1013555886251233No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8880.10033864291985452No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8870.10022564895260243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)