FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093561
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACGGCCGTGTCGTCGGATCTCAGTGTGGTCAGTTCCAAGTAGGCTGTGGTGATGGACGTGTCCCTGGT37980.34730572871563636No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37240.34053884511243543No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT33930.31027075764406375No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC32930.3011263203424409No Hit
GTATAATCACTGGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT31490.287958330628104No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG27380.250374693318434No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24950.22815371067549045No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23850.21809482964370527No Hit
GTCTGGGACAGAGTTCTCTCTCACCATCAGCAGCCTGCAGTCTGAAGATT22540.2061156167785793No Hit
GGCCAAAAGTGTGCCAGTGATTATACTGGTGACAGTAATAAACCGCAAAA22380.20465250681031966No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG22140.20245784185793017No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAATGAAGGTCTCCTGCAAGACTTCTGGATACACCTTCACCG22100.20209206436586527No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG20150.1842604116277007No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCT20140.18416896725468446No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19990.18279730165944105No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCGG19910.18206574667531122No Hit
GTGTACTACTGTGCGAGAGAGGGTTTTACTATGATTCGGGAAAATCGTGC19530.17859086050069453No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGCGGCAACTTAGCCTGGTATCAGCAGAAACCTGGGCAGGCTCCCAGGCT19500.17831652738164583No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19170.1752988630721103No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT18780.17173253252447737No Hit
GCTTGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG18440.16862342384192558No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT17930.16395976081809793No Hit
TGATTATACTGGTGACAGTAATAAACCGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGAGAACTCTGTC17930.16395976081809793No Hit
GTCTGTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCAGGGCCAGTCAGA17620.16112498525459484No Hit
GTTTATTACTGTCACCAGTATAATCACTGGCACACTTTTGGCCAGGGGAC17620.16112498525459484No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17510.16011909715141634No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAATCCTAAAACAGGTGACACAAGCTATG16950.15499821226250754No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16870.1542666572783777No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG16800.1536265466672641No Hit
CCATAGATGAGGAGCCTGGGAGCCTGCCCAGGTTTCTGCTGATACCAGGC16610.15188910357995578No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA15970.14603666370691715No Hit
CAGTAGTACACGGCCGTGTCGTCGGATCTCAGTGTGGTCAGTTCCAAGTA15860.14503077560373862No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA15840.14484788685770617No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC15690.14347622126246273No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15190.13890400261165128No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15060.1377152257624403No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTGCCAGTGATT15060.1377152257624403No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC15050.13762378138942408No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14560.13314300711162888No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTGCCAGTGATTATACTGGTGACAGT14360.13131411965130432No Hit
GAGTTCTCTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCGGTTTATTACTGTCACCAGTATAATCACTGG14000.12802212222272008No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC13960.12765634473065518No Hit
CTTTATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAATCCTAAAACAGGTG13910.12719912286557403No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC13790.1261017903893793No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13600.12436434730207094No Hit
GTCAGAGTCTTAGCGGCAACTTAGCCTGGTATCAGCAGAAACCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTG13560.12399856981000602No Hit
GTAATAAACCGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGAGAACTCTGTCCCAGACCCACTGCC13510.12354134794492488No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGC13280.12143812736555162No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAATCCTAAAACAGGTGACACAAGCTATGCACAGAAGTTTC13090.11970068427824329No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCA12890.1178717968179187No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG12820.11723168620680512No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTTTAGGGCACGATTTTCCCGAATCATAGTAAAACCCTCTCTCG12790.11695735308775643No Hit
ACTTGGAACTGACCACACTGAGATCCGACGACACGGCCGTGTACTACTGT12770.11677446434172398No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC12560.11485413250838317No Hit
CCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGCCC12150.1111049132147178No Hit
CAATGAAGGTCTCCTGCAAGACTTCTGGATACACCTTCACCGACTACTTT12070.11037335823058797No Hit
GTTCCAAGTAGGCTGTGGTGATGGACGTGTCCCTGGTCATGGTGACCCTG11810.107995804532166No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11690.10689847205597126No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11490.10506958459564669No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT11400.10424658523850064No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11330.10360647462738704No Hit
CCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGC11290.10324069713532212No Hit
ATCCTAAAACAGGTGACACAAGCTATGCACAGAAGTTTCAGGGCAGGGTCACCATGACCAGGGACACGTCCATC11200.10241769777817607No Hit
CATCTGTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACT11050.10104603218293264No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC10990.10049736594483526No Hit
GCAGAAACCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTT10980.10040592157181903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)