FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093561
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACGGCCGTGTCGTCGGATCTCAGTGTGGTCAGTTCCAAGTAGGCTGTGGTGATGGACGTGTCCCTGGT42120.385163699144355No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38140.348768838683896No Hit
GTATAATCACTGGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT30830.2819230020090329No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC30320.2772593389852052No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT30230.27643633962805914No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG25090.22943393189771766No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23760.21727183028655922No Hit
GTCTGGGACAGAGTTCTCTCTCACCATCAGCAGCCTGCAGTCTGAAGATT21030.19230751645312882No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20770.18992996275470686No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20550.18791818654834985No Hit
GGCCAAAAGTGTGCCAGTGATTATACTGGTGACAGTAATAAACCGCAAAA20220.18490052223881429No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20110.18389463413563578No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA19990.18279730165944105No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT19590.1791395267387919No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAATGAAGGTCTCCTGCAAGACTTCTGGATACACCTTCACCG19310.1765790842943375No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAATCCTAAAACAGGTGACACAAGCTATG18530.16944642319907166No Hit
GTGTACTACTGTGCGAGAGAGGGTTTTACTATGATTCGGGAAAATCGTGC18370.167983313230812No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18280.16716031387366592No Hit
GCTTGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG18240.16679453638160102No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG18200.1664287588895361No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT17940.16405120519111416No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGCGGCAACTTAGCCTGGTATCAGCAGAAACCTGGGCAGGCTCCCAGGCT17790.16267953959587073No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCT17760.16240520647682205No Hit
TGATTATACTGGTGACAGTAATAAACCGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGAGAACTCTGTC17040.1558212116196536No Hit
GTCTGTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCAGGGCCAGTCAGA16800.1536265466672641No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16760.15326076917519918No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG16750.15316932480218295No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCGG16630.15207199232598823No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC16230.14841421740533908No Hit
GTTTATTACTGTCACCAGTATAATCACTGGCACACTTTTGGCCAGGGGAC16190.14804843991327415No Hit
GTTCCAAGTAGGCTGTGGTGATGGACGTGTCCCTGGTCATGGTGACCCTG15330.1401842238338785No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC15290.13981844634181356No Hit
CAGTAGTACACGGCCGTGTCGTCGGATCTCAGTGTGGTCAGTTCCAAGTA15180.13881255823863506No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15070.13780667013545655No Hit
CCATAGATGAGGAGCCTGGGAGCCTGCCCAGGTTTCTGCTGATACCAGGC14820.13552056081005082No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC14440.13204567463543415No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC14170.12957667656399596No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTTTAGGGCACGATTTTCCCGAATCATAGTAAAACCCTCTCTCG14030.12829645534176876No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC13790.1261017903893793No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTGCCAGTGATTATACTGGTGACAGT13750.12573601289731437No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13740.12564456852429814No Hit
GAGTTCTCTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCGGTTTATTACTGTCACCAGTATAATCACTGG13630.12463868042111963No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC13400.12253545984174637No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC13330.12189534923063278No Hit
GTAATAAACCGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGAGAACTCTGTCCCAGACCCACTGCC13250.12116379424650295No Hit
GTTTTAGGATTGATCCATCCCATCCACTCAAGCCCTTGTCCAGGGGCCTG13110.11988357302427574No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG13060.1194263511591946No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12830.11732313057982134No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA12800.11704879746077265No Hit
ACTTGGAACTGACCACACTGAGATCCGACGACACGGCCGTGTACTACTGT12530.11457979938933449No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTGCCAGTGATT12300.11247657880996123No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12220.1117450238258314No Hit
GTCAGAGTCTTAGCGGCAACTTAGCCTGGTATCAGCAGAAACCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTG12180.11137924633376649No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12160.11119635758773402No Hit
CTTTATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAATCCTAAAACAGGTG11860.10845302639724715No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT11850.10836158202423092No Hit
GTCCTGTGCGAGGCAGCCAACGGCCACGCTGCTTGTATCCGACGGGGAAT11800.10790436015914978No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCA11700.10698991642898749No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATTGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCC11590.10598402832580897No Hit
GTCCAGGGGCCTGTCGCACCCAGTGCATAAAGTAGTCGGTGAAGGTGTAT11560.1057096952067603No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT11410.10433802961151686No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC11090.10141180967499755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)