FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531901
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT23290.43786343699297425No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCC20880.3925542535171018No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC20710.3893581700354013No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT19400.36472952673523834No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19340.36360149727110874No Hit
GATTATTACTGCATCTCATATACAAGCAGCAGCACTTATGTCTTCGGATCTGGGACCAAGGTCACCGTCCTAGGT19020.35758534012908416No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGCGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG18660.3508171633443065No Hit
GACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACATAAGTGCTGCTGCTTGTATATGAGATGCAGTAATAAT17750.3337087164716742No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17500.32900859370446756No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC16860.31697627942041845No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC16850.3167882745097302No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC16700.3139682008494062No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC16020.301183866922604No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC15150.2848274396927248No Hit
ACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACATAAGTGCTGCTGCTTGTATATGAGATGCAGTAATAATC14330.2694110370162869No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCATCTCATAT14250.26790699773078075No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTTCCAACAACACCCAGGCAAAGC14200.2669669731773394No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTTCCAACAAC13050.24534640844818867No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC12860.2417743151451116No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12550.2359461629137753No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12530.2355701530923988No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT12420.23350209907482783No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGCGTCTGGGTCTCCTGGACAGTCGATCAC12340.2319980597893217No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACATAAGTGCTG12040.2263579124686737No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAG12010.22579389773660888No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACATAAGTGCTGCTGCTTGTATATGAGATG11520.2165816571128838No Hit
GGTTATAACTATGTCTCCTGGTTCCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAT11460.2154536276487542No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11450.21526562273806593No Hit
GACTCAGCCTGCCTCCGCGTCTGGGTCTCCTGGACAGTCGATCACCATCT11070.20812143613191175No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG11060.2079334312212235No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10810.20323330845401683No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG9950.18706488613482586No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9600.1804847142607365No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA9300.17484456694008846No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9270.1742805522080237No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9030.16976843435150527No Hit
GCCCTGACTCAGCCTGCCTCCGCGTCTGGGTCTCCTGGACAGTCGATCAC8950.16826439506599913No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG8810.16563232631636338No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG8740.1643162919415455No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG8330.15660809060332656No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8200.15416402676437907No Hit
GTATATGAGATGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC8180.15378801694300254No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAG7960.14965190890786068No Hit
CATAAGTGCTGCTGCTTGTATATGAGATGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTC7910.14871188435441934No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGCGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACC7850.14758385489028972No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC7640.14363575176583612No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT7420.13949964373069426No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCC7310.1374315897131233No Hit
GACTTGGACCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTACTGAC7310.1374315897131233No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACGCGG7250.1363035602489937No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG7170.13479952096348757No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7140.13423550623142277No Hit
GTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAT7120.1338594964100462No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7110.13367149149935798No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCC7050.13254346203522835No Hit
CCCCTGAGGGCCGATTACTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGAACCAGG6990.13141543257109875No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6980.13122742766041048No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGCGTCTGGGTCTCCTGGAC6980.13122742766041048No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT6940.13047540801765742No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG6700.125963290161139No Hit
GTAATACACAGCCGTGTCCTCGGCGGACAGACTGTTCATTTGCAGATACACTGTGTTCTTGGCGTTGTCTCTGG6690.12577528525045073No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC6640.12483526069700941No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTACTG6560.12333122141150327No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCATCTCATATACAAGCAGCAGCACTTATGT6530.12276720667943847No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA6480.12182718212599712No Hit
GCACTTATGTCTTCGGATCTGGGACCAAGGTCACCGTCCTAGGTCAGCCC6350.11938311828704966No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAA6270.11787907900154351No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC6240.11731506426947871No Hit
CTCATATACAAGCAGCAGCACTTATGTCTTCGGATCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCA6110.11487100043053125No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACGCGGAGGCAGG6100.11468299551984297No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGCGTCTGGGT6100.11468299551984297No Hit
GTCCAGGAGACCCAGACGCGGAGGCAGGCTGAGTCAGGGCAGACTGGGCCCAGGACCCTGTGCCCTGAGTGAGG6040.11355496605571337No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCC6010.11299095132364857No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG6010.11299095132364857No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGA5910.11111090221676591No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC5860.11017087766332456No Hit
GGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGGTCC5850.1099828727526363No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC5840.10979486784194804No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC5820.1094188580205715No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT5760.1082908285564419No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG5680.10678678927093575No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGAACCAGGAGACATAGTTATAA5580.10490674016405309No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC5520.10377871069992349No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAG5520.10377871069992349No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG5500.10340270087854694No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC5490.10321469596785869No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC5420.10189866159304081No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGCGTCTGGGTCTCCTGG5410.10171065668235255No Hit
GTTATAACTATGTCTCCTGGTTCCAACAACACCCAGGCAAAGCCCCCAAA5360.1007706321289112No Hit
GGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTACTGACATC5320.10001861248615815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)