FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531901
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT21250.3995104352125678No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCC19760.37149770352001593No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC19590.3683016200383154No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT19240.361721448164226No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC18910.35551728611151323No Hit
GATTATTACTGCATCTCATATACAAGCAGCAGCACTTATGTCTTCGGATCTGGGACCAAGGTCACCGTCCTAGGT18220.3425449472740228No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGCGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG17910.33671679504268653No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC17210.32355645129450783No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG16700.3139682008494062No Hit
GACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACATAAGTGCTGCTGCTTGTATATGAGATGCAGTAATAAT16350.3073880289753168No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC15820.2974237687088387No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15580.29291165085232024No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC14300.268847022284222No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTTCCAACAACACCCAGGCAAAGC14230.26753098790940416No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCATCTCATAT13970.26264286023150923No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGCGTCTGGGTCTCCTGGACAGTCGATCAC13930.26189084058875617No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC12830.24121030041304678No Hit
ACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACATAAGTGCTGCTGCTTGTATATGAGATGCAGTAATAATC12790.24045828077029371No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT12060.2267339222900502No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACATAAGTGCTGCTGCTTGTATATGAGATG12030.22616990755798544No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC11740.22071776514802569No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTTCCAACAAC11550.2171456718449486No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACATAAGTGCTG11140.20943747050672962No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11120.2090614606853531No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10730.20172926916851067No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAG10580.19890919550818667No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10230.19232902363409732No Hit
GGTTATAACTATGTCTCCTGGTTCCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAT10170.19120099416996772No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT10050.18894493524170852No Hit
GACTCAGCCTGCCTCCGCGTCTGGGTCTCCTGGACAGTCGATCACCATCT10040.18875693033102026No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA9600.1804847142607365No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG9490.17841666024316555No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG8950.16826439506599913No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG8880.16694836069118124No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC8750.16450429685223378No Hit
GCCCTGACTCAGCCTGCCTCCGCGTCTGGGTCTCCTGGACAGTCGATCAC8540.16055619372778018No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8260.1552920562285087No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC8210.15435203167506734No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG8200.15416402676437907No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC8190.15397602185369083No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG8140.15303599730024947No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGCGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACC8130.1528479923895612No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7800.1466438303368484No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT7570.14231971739101823No Hit
GTATATGAGATGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC7560.14213171248033No Hit
CATAAGTGCTGCTGCTTGTATATGAGATGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTC7310.1374315897131233No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7300.13724358480243504No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGCGTCTGGGTCTCCTGGAC7280.1368675749810585No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAG7130.1340475013207345No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC7030.13216745221385184No Hit
CCCCTGAGGGCCGATTACTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGAACCAGG6950.13066341292834568No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6850.12878336382146302No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCATCTCATATACAAGCAGCAGCACTTATGT6810.12803134417870995No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC6800.12784333926802166No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG6740.12671530980389206No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG6680.1255872803397625No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6610.12427124596494461No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGCGTCTGGGT6610.12427124596494461No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT6600.12408324105425633No Hit
GACTTGGACCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTACTGAC6520.12257920176875019No Hit
GTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAT6420.12069915266186752No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCC6220.11693905444810218No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG6200.11656304462672563No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC6200.11656304462672563No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG6190.11637503971603738No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCC6170.11599902989466085No Hit
GTAATACACAGCCGTGTCCTCGGCGGACAGACTGTTCATTTGCAGATACACTGTGTTCTTGGCGTTGTCTCTGG6160.1158110249839726No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA6100.11468299551984297No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACGCGG6070.11411898078777817No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCC5910.11111090221676591No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGA5710.10735080400300055No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC5670.1065987843602475No Hit
GCACTTATGTCTTCGGATCTGGGACCAAGGTCACCGTCCTAGGTCAGCCC5620.10565875980680615No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAA5520.10377871069992349No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT5520.10377871069992349No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAG5510.10359070578923521No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC5490.10321469596785869No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC5490.10321469596785869No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGG5440.10227467141441735No Hit
CTCATATACAAGCAGCAGCACTTATGTCTTCGGATCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCA5440.10227467141441735No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCC5370.10095863703959947No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTACTG5340.10039462230753468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)