FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027554
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36050.3508331435622848No Hit
GTATTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCAAGGTCAGCCC33720.32815793622524947No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32440.3157011699628438No Hit
GCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC30530.29711333905566034No Hit
ACATGGGATACCAGCCTGAGTGGTATTGTGGTTTTCGGCGGAGGGACCAA29870.2906903189516074No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA26020.25322270167796535No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23760.23122872374590533No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTC22650.2204263717527254No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC22630.2202317347798753No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC22110.215171173485773No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG21280.20709373911249435No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC21070.2050500508975684No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT20960.2039795475468929No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAGTAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG20940.2037849105740428No Hit
CCACAATACCACTCAGGCTGGTATCCCATGTTGCGCAATAATAGTCGGCC20680.20125462992699167No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC20140.19599943166003927No Hit
GTAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCGGCCACCCTGGGCATC19220.18704613090893518No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19190.18675417544966008No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18490.17994188139990697No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18440.17945528896778173No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC17890.1741027722144043No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17740.17264299491802865No Hit
GTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16780.16330042022122437No Hit
GTTCCTGGGAGCTGCTGGTACCAGGATACATAATTATTTTCAATGTTGAAGCTGCTTCCAGAGCAGGAGATGGTG16100.15668276314432136No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTTCAACATTGAAAATAATTATGTATCCTGGTACCAGC16010.15580689676649598No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA16000.15570957828007093No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15950.1552229858479457No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC15930.15502834887509562No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG15800.15376320855157002No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15710.15288734217374464No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15470.15055169849954358No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15300.1488972842303178No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG15240.14831337331176755No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15030.14626968509684163No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT14910.1451018632597411No Hit
ATCCCATGTTGCGCAATAATAGTCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGGCCGACGTGCC14840.14442063385476578No Hit
GAGTGGTATTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTC14570.14179303472128957No Hit
CCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGACTATTATTGC14450.14062521288418905No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGACTATTATTGCGCAA13470.1310880012145347No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCCGACGTGCCAGACTTGGAGCC13160.12807112813535834No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC13020.12670866932540772No Hit
CTCCTGTACAGCCTCAAAATTCACCTTTGATGATTATGGCATGAGCTGGGTCCGCCAAGCTCCAGGGAAGGGG13010.12661135083898267No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12980.12631939537970754No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT12940.12593012143400736No Hit
ATAATAGTCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGGCCGACGTGCCAGACTTGGAGCCAG12910.12563816597473224No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12860.125151573542607No Hit
ATACCAGCCTGAGTGGTATTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCAAGGTCAGCCCAAGGCTGCCC12610.1227186113819809No Hit
GTTTGGGGGCTGTTCCTGGGAGCTGCTGGTACCAGGATACATAATTATTTTCAATGTTGAAGCTGCTTCCAGAGC12510.12174542651773046No Hit
GCCCCCAAACTCCTCATTTATGACAGTAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT12360.12028564922135479No Hit
ACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATACCACTCAGGCTGGTATCCCATGTTGCGCAATA12350.12018833073492974No Hit
ACTCCAGACTGGGGACGAGGCCGACTATTATTGCGCAACATGGGATACCAGCCTGAGTGGTATTGTGGTTTTCGG11920.11600363581865283No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11790.11473849549512725No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA11610.11298676273947647No Hit
CCCTCAATGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG11300.1099698896603001No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGC11210.1090940232824747No Hit
GGCTGGTATCCCATGTTGCGCAATAATAGTCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGGCCG11000.10705033506754875No Hit
CTCCTGCTCTGGAAGCAGCTTCAACATTGAAAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCC10960.10666106112184859No Hit
ATACCACTCAGGCTGGTATCCCATGTTGCGCAATAATAGTCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCC10940.1064664241489985No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATACC10880.10588251323044823No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC10840.10549323928474805No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10830.10539592079832302No Hit
GTCCTGGGGCCGCAGACATTGAGGGCGGCTGCGTCAACACAGACGGGGCCCAGGACCCTGTGCAGTGAATGAGA10810.10520128382547292No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10800.10510396533904788No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10690.10403346198837239No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT10650.10364418804267221No Hit
CTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTT10470.1018924552870214No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10430.10150318134132125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)