FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027554
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34430.33506754876142764No Hit
GTATTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCAAGGTCAGCCC33230.32338933039042234No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30740.29915702727058624No Hit
ACATGGGATACCAGCCTGAGTGGTATTGTGGTTTTCGGCGGAGGGACCAA25810.2511790134630394No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA25150.24475599335898648No Hit
GTCCTGGGCCCCGTCTGTGTTGACGCAGCCGCCCTCAATGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG23760.23122872374590533No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC23030.2241244742368771No Hit
GCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC22580.21974514234775008No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC21760.21176502646089645No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21600.21020793067809576No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT20600.20047608203559134No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC19920.1938584249586883No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC18890.18383462085690874No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18770.1826667990198082No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG18740.18237484356053307No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAGTAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG18600.18101238475058246No Hit
GTAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCGGCCACCCTGGGCATC18230.1774116007528558No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTTGGACGGTCAGCTTGGTCCCTC18150.17663305286145545No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17570.1709885806488029No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17190.1672904781646512No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG16250.15814254044069703No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16200.1576559480085718No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16030.15600153373934605No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15960.15532030433437075No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTTCAACATTGAAAATAATTATGTATCCTGGTACCAGC15820.15395784552442013No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC15480.15064901698596864No Hit
CCACAATACCACTCAGGCTGGTATCCCATGTTGCGCAATAATAGTCGGCC15440.15025974304026846No Hit
GTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15360.14948119514886807No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG15100.14695091450181694No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14910.1451018632597411No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14600.14208499018056472No Hit
GAGTGGTATTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCAAGGTCAGCCCAAGGCTGCCCCCTCGGTC14470.14081984985703913No Hit
GTGTTGACGCAGCCGCCCTCAATGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTTC14400.14013862045206385No Hit
CTCCTGTACAGCCTCAAAATTCACCTTTGATGATTATGGCATGAGCTGGGTCCGCCAAGCTCCAGGGAAGGGG14210.138289569209988No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC13890.13517537764438659No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA13740.13371560034801092No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT13470.1310880012145347No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGACTATTATTGCGCAA13460.13099068272810968No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13340.12982286089100914No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13010.12661135083898267No Hit
CCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGACTATTATTGC12710.12369179624623133No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12620.12281592986840593No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT12410.12077224165348002No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG12090.1176580500878786No Hit
CTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTT11920.11600363581865283No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC11850.11532240641367753No Hit
ATCCCATGTTGCGCAATAATAGTCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGGCCGACGTGCC11830.11512776944082743No Hit
ATACCAGCCTGAGTGGTATTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCAAGGTCAGCCCAAGGCTGCCC11710.11395994760372691No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCCGACGTGCCAGACTTGGAGCC11710.11395994760372691No Hit
GTTCCTGGGAGCTGCTGGTACCAGGATACATAATTATTTTCAATGTTGAAGCTGCTTCCAGAGCAGGAGATGGTG11690.11376531063087682No Hit
CCCTCAATGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG11690.11376531063087682No Hit
GGGTCCTGGGCCCCGTCTGTGTTGACGCAGCCGCCCTCAATGTCTGCGGCCCCAGGACAGAAGGTCACCATCTC11650.11337603668517664No Hit
ACTCCAGACTGGGGACGAGGCCGACTATTATTGCGCAACATGGGATACCAGCCTGAGTGGTATTGTGGTTTTCGG11470.11162430392952585No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11270.10967793420102495No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11030.1073422905268239No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10990.10695301658112372No Hit
GCCCCCAAACTCCTCATTTATGACAGTAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT10970.10675837960827361No Hit
CTCCTGCTCTGGAAGCAGCTTCAACATTGAAAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCC10870.10578519474402318No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT10840.10549323928474805No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10570.10286564015127186No Hit
GCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGA10490.1020870922598715No Hit
GTCCTGGGGCCGCAGACATTGAGGGCGGCTGCGTCAACACAGACGGGGCCCAGGACCCTGTGCAGTGAATGAGA10370.10091927042277096No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA10360.10082195193634592No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10300.10023804101779567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)