FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616262
Sequences flagged as poor quality0
Sequence length19-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG26450.4292005672911846No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25880.4199512544988982No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCCCCGCCGAAAACCACATAACCCTTCAGGCTGTCATCCCA25250.4097283298337396No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG25070.4068074942151228No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG24590.3990185992321448No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCA24580.3988563305866661No Hit
GACCTAGGACGGTCAGCCTGGTCCCCCCGCCGAAAACCACATAACCCTTC23320.3784104812563488No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22410.36364403451778626No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21570.35001346829757474No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20130.3266467833486407No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC19330.31366529171034396No Hit
CCCCTGAGGGCCGCCGATTATTAGCATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA18210.2954912034167286No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCCC17800.2888381889521015No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17650.2864041592699209No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17400.2823474431329532No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC16620.2696904887856139No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16380.2657960412941249No Hit
CCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAG15700.2547617734015727No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC15590.2529768183013069No Hit
GAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA15040.24405204279997791No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14760.23950852072657408No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14660.237885834271787No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14270.23155735709811734No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14080.2284742528340219No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13800.22393073076061804No Hit
ACCTAGGACGGTCAGCCTGGTCCCCCCGCCGAAAACCACATAACCCTTCA13680.22198350701487357No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13660.2216589697239161No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13390.21727771629599096No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13270.2153304925502465No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA12730.20656798569439624No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12620.2047830305941304No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG12360.200564045811684No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA11710.1900165838555679No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG11670.1893675092736531No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11610.18839389740078083No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT11510.18677121094599375No Hit
CTCTTGTTCTGGAAGCACCTCCAATATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCC11300.18336356939094087No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG11040.17914458460849442No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11030.17898231596301573No Hit
ACATGGGATGACAGCCTGAAGGGTTATGTGGTTTTCGGCGGGGGGACCAG10920.17719736086274993No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10920.17719736086274993No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10840.17589921169892025No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10830.17573694305344156No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10820.17557467440796284No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10810.17541240576248415No Hit
GTATTACTTCCGATATTGGAGGTGCTTCCAGAACAAGAGATGGTGACCCT10690.17346518201673963No Hit
ATAATAATCACCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGG10490.17021980910716547No Hit
CCCAGGAACGGCCCCCAAACTCCTCATTTATGCTAATAATCGGCGGCCCTCAGGGGTCCCTGACCGATTTTC10190.1653517497428042No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGG9990.16210637683323No Hit
CCACATAACCCTTCAGGCTGTCATCCCATGTTGAACAATAATAATCACCCTCATCCTCAGACTGGAGTCCACTGA9920.16097049631487906No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT9860.15999688444200683No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGTCTGAGGATGAGGGTGATTATTATTGTTCAACA9820.15934780986009198No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG9740.1580496606962623No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC9570.15529109372312425No Hit
TCCTCAGACTGGAGTCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAAAATCGGTCAGGGACC9530.15464201914120942No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9460.15350613862285845No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGG9110.14782673603110366No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC9050.1468531241582314No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC9030.146528586867274No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9010.14620404957631658No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8670.1406869156300405No Hit
GTTATGTGGTTTTCGGCGGGGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGT8620.13987557240264692No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8580.1392264978207321No Hit
CCCTTCAGGCTGTCATCCCATGTTGAACAATAATAATCACCCTCATCCTC8450.13711700542950886No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTG8450.13711700542950886No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT8370.1358188562656792No Hit
ACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCAT8270.1341961698108921No Hit
GCCCCCAAACTCCTCATTTATGCTAATAATCGGCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAG8200.13306028929254116No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTC8150.13224894606514762No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA8140.13208667741966892No Hit
CCTCCAATATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATTTA8120.13176214012871149No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACT8110.13159987148323277No Hit
GGGTCACCATCTCTTGTTCTGGAAGCACCTCCAATATCGGAAGTAATACT8100.13143760283775408No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8020.1301394536739244No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG8000.129814916382967No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7930.12867903586461601No Hit
GTGCCAGACTTGGAGCCAGAAAATCGGTCAGGGACCCCTGAGGGCCGCCGATTATTAGCATAAATGAGGAGTT7930.12867903586461601No Hit
GTCTGAGGATGAGGGTGATTATTATTGTTCAACATGGGATGACAGCCTGA7890.1280299612827012No Hit
TCAGTGGACTCCAGTCTGAGGATGAGGGTGATTATTATTGTTCAACATGGGATGACAGCCTGAAGGGTTATGTGG7820.12689408076435024No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG7760.12592046889147798No Hit
ATCCCATGTTGAACAATAATAATCACCCTCATCCTCAGACTGGAGTCCAC7730.12543366295504185No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA7660.12429778243669089No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGTATTACTT7650.12413551379121218No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7590.12316190191833992No Hit
CTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATT7560.1226750959819038No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7460.12105240952711672No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCCCCGCCGAAAACCACATAACCCT7410.12024106629972317No Hit
GGCCATCAGTGGACTCCAGTCTGAGGATGAGGGTGATTATTATTGTTCAACATGGGATGACAGCCTGAAGGGTTA7380.11975426036328704No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACT7330.1189429171358935No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7290.11829384255397866No Hit
GCCTGGTCCCCCCGCCGAAAACCACATAACCCTTCAGGCTGTCATCCCATGTTGAACAATAATAATCACCCTCA7290.11829384255397866No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCACCTCCAATATCGGAAGTAATACTGTAAA7270.11796930526302125No Hit
CCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGGCTGAGGTGCC7250.11764476797206383No Hit
GTCATCCCATGTTGAACAATAATAATCACCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGGCTGAGG7210.116995693390149No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC7210.116995693390149No Hit
GATTATTATTGTTCAACATGGGATGACAGCCTGAAGGGTTATGTGGTTTTCGGCGGGGGGACCAGGCTGACCGTC7170.11634661880823416No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7090.11504846964440447No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG6940.11261443996222387No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAG6930.11245217131674516No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAAAATCGGTCAGGGACCCCTGAGGGCCGCCGATTA6920.11228990267126643No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG6910.11212763402578772No Hit
GTTCTGGAAGCACCTCCAATATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA6890.11180309673483031No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC6880.11164082808935161No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG6820.11066721621647935No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT6810.11050494757100064No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC6780.11001814163456453No Hit
GCCCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAGTCTGGCACCTCAG6750.10953133569812838No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAG6730.10920679840717097No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAC6660.10807091788882No Hit
GGAGTCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAA6450.10466327633376712No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG6420.10417647039733101No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCA6400.10385193310637358No Hit
TGGGATGACAGCCTGAAGGGTTATGTGGTTTTCGGCGGGGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGG6380.10352739581541617No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT6220.10093109748775682No Hit
GGCAGAGGGTCACCATCTCTTGTTCTGGAAGCACCTCCAATATCGGAAGTAATACTGTAAACTGGTACCAGCAG6220.10093109748775682No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGG6210.10076882884227811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)