FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616262
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT28370.4603561472230967No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC26500.4300119105185782No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG24530.3980449873592726No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23260.3774368693834765No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCCCCGCCGAAAACCACATAACCCTTCAGGCTGTCATCCCAT22590.36656487013640304No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22430.3639685718087437No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC21720.35244749797975533No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT21160.34336045383294767No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18150.29451759154385637No Hit
GACCTAGGACGGTCAGCCTGGTCCCCCCGCCGAAAACCACATAACCCTTC17730.2877023084337506No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCA17620.2859173533334848No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG17220.27942660751433646No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA16710.2711509065949223No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA16670.2705018320130074No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCCC16530.2682300709763055No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16250.26368654890290166No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA15690.254599504756094No Hit
CCCCTGAGGGCCGCCGATTATTAGCATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA15530.25200320642843466No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA15140.24567472925476502No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG14950.24259162499066952No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG14930.2422670876997121No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14430.23415365542577668No Hit
GAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATTTATGCTAATAATCGGC14250.23123281980715993No Hit
CCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGT14160.22977240199785154No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13820.22425526805157545No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCA13210.2143568806773742No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12860.20867747808561943No Hit
CTCTTGTTCTGGAAGCACCTCCAATATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCC11930.1935864940560995No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11910.1932619567651421No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT11750.19066565843748276No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT11700.18985431521008922No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11630.18871843469173824No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA11420.1853107931366854No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11280.18303903209998346No Hit
ACCTAGGACGGTCAGCCTGGTCCCCCCGCCGAAAACCACATAACCCTTCA11240.1823899575180686No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC10910.17703509221727123No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG10700.17362745066221832No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG10620.17232930149838865No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10510.17054434639812288No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGG10330.16762351077950613No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10220.16583855567924033No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA10150.16470267516088935No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10140.16454040651541066No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10080.1635667946425384No Hit
GTATTACTTCCGATATTGGAGGTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAG10060.163242257351581No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA10040.16291772006062358No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC9780.15869873527817716No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG9490.15399294455929458No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC9470.15366840726833716No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTA9390.1523702581045075No Hit
GACCTAGGACGGTCAGCCTGGTCCCCCCGCCGAAAACCACATAACCCTTCAGGCTGTCATCCCATGTTGAACAAT9280.1505853030042417No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC9210.14944942248589074No Hit
ATAATAATCACCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGG9090.14750219874014625No Hit
CCACATAACCCTTCAGGCTGTCATCCCATGTTGAACAATAATAATCACCCTCATCCTCAGACTGGAGTCCACT9080.14733993009466753No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT9050.1468531241582314No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAA8830.14328321395769983No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG8830.14328321395769983No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT8790.14263413937578498No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGTCTGAGGATGAGGGTGATTATTATTGTTCAACATG8760.14214733343934885No Hit
TCCTCAGACTGGAGTCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAAAATCGGTCAGGGACC8740.14182279614839144No Hit
ACATGGGATGACAGCCTGAAGGGTTATGTGGTTTTCGGCGGGGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAG8710.1413359902119553No Hit
CCCAGGAACGGCCCCCAAACTCCTCATTTATGCTAATAATCGGCGGCCCTCAGGGGTCCCTGACCGATTTTCTG8620.13987557240264692No Hit
ACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATTTATGCTAATAATCGGCGGCCCTCA8510.13809061730238112No Hit
GTTATGTGGTTTTCGGCGGGGGGACCAGGCTGACCGTCCTAGGTCAGCCC8500.13792834865690243No Hit
CCCTTCAGGCTGTCATCCCATGTTGAACAATAATAATCACCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGG8400.13630566220211535No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGT8250.1338716325199347No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT8150.13224894606514762No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8040.13046399096488182No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT7880.12786769263722247No Hit
TCAGTGGACTCCAGTCTGAGGATGAGGGTGATTATTATTGTTCAACATGG7870.12770542399174378No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7830.12705634940982893No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTC7800.1265695434733928No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7780.1262450061824354No Hit
GGGTCACCATCTCTTGTTCTGGAAGCACCTCCAATATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAG7770.12608273753695667No Hit
ATCCCATGTTGAACAATAATAATCACCCTCATCCTCAGACTGGAGTCCAC7740.12559593160052057No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7700.12494685701860574No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA7690.124784588373127No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC7660.12429778243669089No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7650.12413551379121218No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG7640.12397324514573348No Hit
CCTCCAATATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATTTAT7610.12348643920929733No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGTATTACTT7460.12105240952711672No Hit
GCCCCCAAACTCCTCATTTATGCTAATAATCGGCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAGT7450.12089014088163802No Hit
GATTATTATTGTTCAACATGGGATGACAGCCTGAAGGGTTATGTGGTTTTCGGCGGGGGGACCAGGCTGACCGTC7400.12007879765424447No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC7350.11926745442685091No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7320.1187806484904148No Hit
GTCTGAGGATGAGGGTGATTATTATTGTTCAACATGGGATGACAGCCTGAAGGGTTATGTGGTTTTCGGCGGGGG7310.11861837984493609No Hit
GTGCCAGACTTGGAGCCAGAAAATCGGTCAGGGACCCCTGAGGGCCGCCGATTATTAGCATAAATGAGGAGTTTG7290.11829384255397866No Hit
GTGGACTCCAGTCTGAGGATGAGGGTGATTATTATTGTTCAACATGGGAT7260.11780703661754253No Hit
GTTCTGGAAGCACCTCCAATATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA7230.1173202306811064No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCA7170.11634661880823416No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA7140.11585981287179803No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7040.11423712641701095No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA7010.11375032048057483No Hit
CCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAAAATCGGTCA6980.11326351454413869No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCA6910.11212763402578772No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGG6890.11180309673483031No Hit
GCCTGGTCCCCCCGCCGAAAACCACATAACCCTTCAGGCTGTCATCCCATGTTGAACAATAATAATCACCCTCAT6830.11082948486195805No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCA6810.11050494757100064No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAC6810.11050494757100064No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTG6770.10985587298908582No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCCCCGCCGAAAAC6760.10969360434360712No Hit
GTCATCCCATGTTGAACAATAATAATCACCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGGCTGAGGT6690.10855772382525615No Hit
GGCCATCAGTGGACTCCAGTCTGAGGATGAGGGTGATTATTATTGTTCAA6630.10758411195238389No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCACCTCCAATATCGGAAGTAATACTGTAAA6620.10742184330690518No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAG6600.10709730601594777No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT6600.10709730601594777No Hit
CTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATTTATGCTAATAATCGGCGGCCCTCAG6360.10320285852445875No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGG6260.10158017206967167No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG6240.10125563477871426No Hit
TGGGATGACAGCCTGAAGGGTTATGTGGTTTTCGGCGGGGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAG6230.10109336613323552No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAAC6230.10109336613323552No Hit
GCCCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCA6170.10011975426036329No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)