FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295910
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62220.48012593467138925No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT54380.41962790625892227No Hit
GAGTTACAGTATCTCGTACACTTTTGGCCAGGGGACCAAGCTGCATATCAAACGAACTGTGGCTGCACCAT40760.3145280150627744No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39850.3075059224791845No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG37890.29238141537606777No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35940.27733407412551797No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG34690.2676883425546527No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33400.2577339475735198No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG32850.2534898256823391No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAA32050.24731655747698528No Hit
GATCTATGCAGCATCCGTTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCGGGGACAGATTTC31700.244615752637143No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29430.22709910410445172No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC29000.22378097244407402No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC28410.21922818714262565No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG27850.21490689939887803No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC26940.2078848068152881No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA26040.2009398800842651No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA25720.1984705728021236No Hit
CTTTTGGCCAGGGGACCAAGCTGCATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG25520.19692725575078518No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGT25420.19615559722511597No Hit
GTGCAGCCACAGTTCGTTTGATATGCAGCTTGGTCCCCTGGCCAAAAGTGTACGAGATACTGTAACTCTGTTGAC24750.19098548510313218No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT24140.1862783680965499No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT23830.18388622666697532No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC23750.18326889984643996No Hit
GTAATATAGGGCCGTGTCGTCAGATCTCAGGCTGCTCAGTTCCATGTAGACTGTGCTGTTGGATTCGTCCGCGGT23520.18149408523740074No Hit
GGCCAAAAGTGTACGAGATACTGTAACTCTGTTGACAGTAGTAAGTTGCA23500.1813397535322669No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23050.1778672901667554No Hit
ACAGTATCTCGTACACTTTTGGCCAGGGGACCAAGCTGCATATCAAACGA22990.17740429505135388No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22220.17146252440370088No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATGCAGCTTGGTCCCCTGGCCAAAAGTGTACGAGAT21400.16513492449321326No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21150.1632057781790402No Hit
ACTTACTACTGTCAACAGAGTTACAGTATCTCGTACACTTTTGGCCAGGGGACCAAGCTGCATATCAAACGA20480.15803566605705643No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAT20470.1579585002044895No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20420.1575726709416549No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19780.15263405637737187No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCCGATCCACTGCC19730.15224822711453728No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA19690.1519395637042696No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT19520.1506277442106319No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC19390.14962458812726193No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC19230.14838993448619117No Hit
GCATAGATCAGTAACTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT19140.14769544181308888No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18940.14615212476175043No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC18890.14576629549891582No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18540.14306549065907354No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC18480.14260249554367202No Hit
GTTCAGTGGCAGTGGATCGGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTT18040.13920719803072745No Hit
AGATACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT17530.13527173954981442No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17390.1341914176138775No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC17310.13357409079334215No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC17110.1320307737420037No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17060.13164494447916908No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16700.1288669737867599No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT16580.12794098355595682No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16440.1268606616200199No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC16420.12670632991488606No Hit
CCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT16300.125780339684083No Hit
ATATTACTGTGCGAGACGGGGGAAAGATGGCCACTGGTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCAC16130.12446852019044531No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC15990.12338819825450842No Hit
AGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTACTGATCTATGC15930.12292520313910689No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15840.12223071046600457No Hit
TCTATGCAGCATCCGTTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCGGGGACAGATTTCACT15840.12223071046600457No Hit
ATGCAGCATCCGTTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCGGGGACAGATTTCACTCTC15710.1212275543826346No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15660.1208417251198No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT15610.12045589585696537No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATG15040.11605744226065082No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC15000.11574877885038312No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC14830.11443695935674546No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14810.1142826276516116No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATC14710.11351096912594238No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTATCTCGTACACTTTTGGCCAGGGG14630.11289364230540702No Hit
GCCCCTAAGTTACTGATCTATGCAGCATCCGTTTTGCAAAGTGGGGTCCC14500.11189048622203701No Hit
GAGTGAAATCTGTCCCCGATCCACTGCCACTGAACCTTGACGGGACCCCACTTTGCAAAACGGATGCTGCAT14440.1114274911066355No Hit
CAGTAATATAGGGCCGTGTCGTCAGATCTCAGGCTGCTCAGTTCCATGTA14080.1086495204142263No Hit
GTTACTGATCTATGCAGCATCCGTTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCGGGGAC14050.10841802285652553No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14040.10834085700395862No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13790.10641171068978557No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCGGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTG13760.1061802131320848No Hit
GATCAGTAACTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT13750.10610304727951786No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC13710.10579438386925018No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG13690.10564005216411633No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT13670.10548572045898248No Hit
GCTTGGTCCCCTGGCCAAAAGTGTACGAGATACTGTAACTCTGTTGACAG13550.10455973022817944No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13350.10301641317684099No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG13290.10255341806143946No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATT13190.10178175953577022No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13180.1017045936832033No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGC13150.10147309612550254No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13080.10093293515753408No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12960.10000694492673104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)