FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295910
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60950.4703258713953901No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT48050.3707819215840606No Hit
GAGTTACAGTATCTCGTACACTTTTGGCCAGGGGACCAAGCTGCATATCAAACGAACTGTGGCTGCACCAT38340.2958538787415793No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35320.2725497912663688No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34970.26984898642652655No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31680.24446142093200915No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31300.24152911853446615No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA30810.23774799175868694No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG29630.22864242115579014No Hit
GATCTATGCAGCATCCGTTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCGGGGACAGATTTC28430.2193825188477595No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC28220.21776203594385413No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAA27860.21498406525144492No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26520.2046438410074774No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC26390.2036406849241074No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25200.19445794846864364No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGT25060.19337762653270674No Hit
GTAATATAGGGCCGTGTCGTCAGATCTCAGGCTGCTCAGTTCCATGTAGACTGTGCTGTTGGATTCGTCCGCGGT24470.18882484123125834No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG24240.18705002662221915No Hit
GTGCAGCCACAGTTCGTTTGATATGCAGCTTGGTCCCCTGGCCAAAAGTGTACGAGATACTGTAACTCTGTTGAC24110.18604687053884914No Hit
CTTTTGGCCAGGGGACCAAGCTGCATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG24100.1859697046862822No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC23270.1795649389232277No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG22760.17562948044231466No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC22710.17524365117948004No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22650.17478065606407853No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22520.17377749998070852No Hit
GGCCAAAAGTGTACGAGATACTGTAACTCTGTTGACAGTAGTAAGTTGCA22100.17053653417289782No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT21760.16791289518562244No Hit
ACAGTATCTCGTACACTTTTGGCCAGGGGACCAAGCTGCATATCAAACGA21070.16258845135850483No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT20950.16166246112770177No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20860.16096796845459946No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC19980.15417737342871032No Hit
ACTTACTACTGTCAACAGAGTTACAGTATCTCGTACACTTTTGGCCAGGGGACCAAGCTGCATATCAAACGA19940.15386871001844266No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATGCAGCTTGGTCCCCTGGCCAAAAGTGTACGAGAT19680.15186239785170266No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT19670.15178523199913574No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCCGATCCACTGCC19570.15101357347346653No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19490.15039624665293114No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC19460.1501647490952304No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAT19090.14730961255025427No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA18980.14646078817201813No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18670.14406864674244355No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCGGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTG18390.1419080028705697No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18380.1418308370180028No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC18290.1411363443449005No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA17870.13789537853708977No Hit
GCATAGATCAGTAACTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT17580.13565756881264904No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC17270.13326542738307445No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC17210.1328024322676729No Hit
ATATTACTGTGCGAGACGGGGGAAAGATGGCCACTGGTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCAC16900.13041029083809833No Hit
AGATACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT16600.12809531526109066No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16570.1278638177033899No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT16410.12662916406231914No Hit
GTTCAGTGGCAGTGGATCGGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTT16240.12531734456868146No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC15630.12061022756209923No Hit
CCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT15600.12037873000439844No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15590.12030156415183152No Hit
AGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTACTGATCTATGC15530.11983856903642999No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC15440.1191440763633277No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT15370.11860391539535925No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15320.11821808613252463No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15220.11744642760685542No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC15010.11582594470295005No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTATCTCGTACACTTTTGGCCAGGGG14830.11443695935674546No Hit
ATGCAGCATCCGTTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCGGGGACAGATTTCACTCTC14730.11366530083107623No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14630.11289364230540702No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14530.11212198377973778No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC14480.11173615451690318No Hit
TCTATGCAGCATCCGTTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCGGGGACAGATTTCACT14410.11119599354893472No Hit
CAGTAATATAGGGCCGTGTCGTCAGATCTCAGGCTGCTCAGTTCCATGTA14290.11027000331813165No Hit
GAGTGAAATCTGTCCCCGATCCACTGCCACTGAACCTTGACGGGACCCCACTTTGCAAAACGGATGCTGCAT14140.10911251552962783No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG14000.10803219359369091No Hit
GTTACTGATCTATGCAGCATCCGTTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCGGGGAC13980.10787786188855707No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCCGATCCACTGCCACTGAACCT13510.10425106681791174No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13500.10417390096534483No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13460.10386523755507712No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATC13460.10386523755507712No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATG13090.10101010101010101No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATT13030.10054710589469948No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC13010.10039277418956565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)