FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001655
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT29780.29730795533392235No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC25910.2586718980087954No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA23830.23790626513120783No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATGAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG22420.22382956207476623No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC22180.2214335275119677No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21320.21284773699527282No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCACCTG21310.21274790222182288No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21310.21274790222182288No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG21240.21204905880767333No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC21200.21164971971387353No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20740.2070573201351763No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC20140.20106723372817986No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCACCTGCTCTGGAAGCAGCTCC18800.18768937408588784No Hit
ACCCTGGACATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTG18160.18129994858509169No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT17760.17730655764709405No Hit
GTACCAGCACCTCCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATGAGCGACCCTCAGGGATTCCTG17550.1752100274046453No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC16260.162331341629603No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT15960.1593362984261048No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15190.1516490208704594No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15010.14985199494836046No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG14690.14665728219796237No Hit
GTTCCTGGGAGGTGCTGGTACCAGGCCACATAATTATTCCCAATGTTGGAGCTGCTTCCAGAGCAGGTGATGGTG14510.14486025627586344No Hit
GACCTACGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAGAGGGCACTCAGGCTGTTATCCCATGTTCCGCAGT14350.1432628999006644No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14330.1430632303537645No Hit
GCCTTGGGCTGACCTACGACGGTGACCTTGGTCCCAGTTCCGAAGACAAA13770.13747248304056786No Hit
TCGTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13760.1373726482671179No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTACGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAGAGGGC13360.13337925732912032No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13180.13158223140702138No Hit
GGACAGAAGGTCACCATCACCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTGGCCTGGTACCAGCAC12670.12649065796107442No Hit
GATTATTACTGCGGAACATGGGATAACAGCCTGAGTGCCCTCTTTGTCTT12480.12459379726552555No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA12020.12000139768682831No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC12020.12000139768682831No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG12010.11990156291337836No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT11960.11940238904612865No Hit
GTCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCTGAGGGTCGCTCATTATTGTC11860.11840404131162927No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCACCTGCTCTGG11670.11650718061608038No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC11490.11471015469398146No Hit
ACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGACATCACCGGACTCC11410.11391147650638195No Hit
GTAATAAACGGCCGTGTCCGCGGCGGTCACAGAGTTCAGCCTCAGGGAGAACTGGTTCCTGGACGTGTCTACTG11290.11271345922498266No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG11210.11191478103738312No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11200.1118149462639332No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTGTGGTCCCACTCGTATATGGTGGTG11160.11141560717013343No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG11140.11121593762323356No Hit
ACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAACAGCCTGAGTGCCCTCTTTGTCTTCGG10830.10812105964628539No Hit
TCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGG10600.10582485985693676No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG10550.10532568598968707No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG10530.1051260164427872No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAG10490.10472667734898741No Hit
GGATAACAGCCTGAGTGCCCTCTTTGTCTTCGGAACTGGGACCAAGGTCACCGTCGTAGGTCAGCCCAAGGCCA10470.10452700780208754No Hit
CTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTT10360.1034288252941382No Hit
GTGCTGGTACCAGGCCACATAATTATTCCCAATGTTGGAGCTGCTTCCAGAGCAGGTGATGGTGACCTTCTGTC10310.1029296514268885No Hit
GTCTGGAGTCCGGTGATGTCCAGGGTGGCTGACGTGCCAGACTTGGAGCC10280.10263014710653869No Hit
GTCGTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC10170.10153196459858935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)