FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302295
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT77060.5917246092475207No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT71180.5465735490038739No Hit
GATCTATGCTGCAACCAATTTGCAAGGTGGGGTCCCACCTAGGTTCAGTG54400.417724094771154No Hit
GTACTATTGTCAACAGTCTAACGGATATCCTCGGACGTTCGGCCCAGGGA51950.3989111530029678No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC49230.37802494826441013No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48750.3743391474281941No Hit
ATTAATACCTGGTTAGCCTGGTATCAGCACAAACCTGGGAAAGCCCCTAG46460.3567548059387466No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG43990.33778828913571807No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42500.3263469490399641No Hit
CCGTTAGACTGTTGACAATAGTACGTTGCAAAATCCTCAGGCTGGAGGTTGCTGATTGTGAGACTGAAATCTGTT41870.32150933544243043No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA41210.3164413592926334No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG40770.313062708526102No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40670.3122948333518903No Hit
GTCTAACGGATATCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT40630.3119876832822056No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT37980.29163899116559616No Hit
CAATAGTACGTTGCAAAATCCTCAGGCTGGAGGTTGCTGATTGTGAGACTGAAATCTGTTCCAGAGCCACTGCC37780.29010324081717276No Hit
ATTGTCAACAGTCTAACGGATATCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTG37050.2844977520454275No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA36900.28334593928411No Hit
GTTGACAATAGTACGTTGCAAAATCCTCAGGCTGGAGGTTGCTGATTGTGAGACTGAAATCTGTTCCAGAGCC36120.27735651292525887No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC35700.2741314371935698No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG33260.25539528294280484No Hit
GTCTCCATCCTCTGTGTCTGCGTCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGTCTATTAATAC32940.25293808238532745No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA31190.239500266836623No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTGGGCCGAACGTCCGAGGATATCCGTTAGACTGTTGAC31100.23880917917983255No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC30690.23566089096556464No Hit
GTCTATTAATACCTGGTTAGCCTGGTATCAGCACAAACCTGGGAAAGCCCCTAGGCCCCTGATCTATGCTGCAAC30390.2333572654429296No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT30190.23182151509450621No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28570.21938193727227703No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28140.2160800740231668No Hit
GCATAGATCAGGGGCCTAGGGGCTTTCCCAGGTTTGTGCTGATACCAGGC28120.21592649898832444No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC27200.208862047385577No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26600.2042547963403069No Hit
ACCTAGGTGGGACCCCACCTTGCAAATTGGTTGCAGCATAGATCAGGGGCCTAGGGGCTTTCCCAGGTTTGTG26570.2040244337880434No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC25800.19811179494661346No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25650.19695998218529598No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC25460.1955010193542938No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC24760.190125893134812No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24430.18759190505991347No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24350.18697760492054413No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT24200.1858257921592266No Hit
CCTCCAGCCTGAGGATTTTGCAACGTACTATTGTCAACAGTCTAACGGATATCCTCGGACGTTCGGCCCAGGGAC23490.18037387842232366No Hit
GCCCAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGC23320.1790684906261638No Hit
GGCTGGAGGTTGCTGATTGTGAGACTGAAATCTGTTCCAGAGCCACTGCC23180.17799346538226746No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23160.17783989034742512No Hit
CACCTAGGTTCAGTGGCAGTGGCTCTGGAACAGATTTCAGTCTCACAATCAGCAACCTCCAGCCTGAGGATTTTG23010.1766880775861076No Hit
CCCCTGATCTATGCTGCAACCAATTTGCAAGGTGGGGTCCCACCTAGGTT22420.1721576140582587No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC21820.16755036301298862No Hit
GGTATTAATAGACTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCTACAGACGCAGACACAGAGGATGG21710.16670570032135576No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21590.16578425011230175No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC21540.1654003125251959No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT21490.16501637493809007No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC21370.16409492472903606No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21370.16409492472903606No Hit
GTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGTCTATTAATACCTGGTTAGCCTGGTATCAGCACAA20690.15887337354439662No Hit
GCACAAACCTGGGAAAGCCCCTAGGCCCCTGATCTATGCTGCAACCAATTTGCAAGGTGGGGTCCCACCTAGGTT20260.1555715102952864No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20080.15418933498170537No Hit
GATCAGGGGCCTAGGGGCTTTCCCAGGTTTGTGCTGATACCAGGCTAACCAGGTATTAATAGACTGACTCGCCCG19980.1534214598074937No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC19670.1510410467674375No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19310.14827669614027544No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18780.14420695771695352No Hit
CTATTAATACCTGGTTAGCCTGGTATCAGCACAAACCTGGGAAAGCCCCTAGGCCCCTGATCTATGCTGCAACCA18680.14343908254274185No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18650.14320871999047835No Hit
GGTTTGTGCTGATACCAGGCTAACCAGGTATTAATAGACTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCT18460.14174975715947616No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT18120.13913898156715643No Hit
GTGTCTGCGTCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCA18040.1385246814277871No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC17810.13675856852710025No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAGATG17700.1359139058354674No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17460.13407100541735936No Hit
CCTAGGTTCAGTGGCAGTGGCTCTGGAACAGATTTCAGTCTCACAATCAGCAACCTCCAGCCTGAGGATTTTGCA17400.13361028031283234No Hit
AACCTGGGAAAGCCCCTAGGCCCCTGATCTATGCTGCAACCAATTTGCAA17310.13291919265604185No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC17200.132074529964409No Hit
TTAATAGACTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCTACAGA16790.1289262417501411No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16620.12762085395398123No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16470.12646904119266372No Hit
GTCCCTGGGCCGAACGTCCGAGGATATCCGTTAGACTGTTGACAATAGTA16260.12485650332681919No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16190.12431899070487103No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16180.12424220318744984No Hit
GTGGGGTCCCACCTAGGTTCAGTGGCAGTGGCTCTGGAACAGATTTCAGTCTCACAATCAGCAACCTCCAGCCTG15940.12239930276934181No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA15810.12140106504286664No Hit
ACCCAGTCTCCATCCTCTGTGTCTGCGTCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGTCTATT15680.12040282731639144No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC15620.11994210221186444No Hit
AGGTGGGACCCCACCTTGCAAATTGGTTGCAGCATAGATCAGGGGCCTAGGGGCTTTCCCAGGTTTGTGCTGAT15520.11917422703765276No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15420.11840635186344108No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15360.11794562675891407No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15270.11725453910212356No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC15220.11687060151501771No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15210.11679381399759656No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15000.11518127613175203No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG14930.11464376350980385No Hit
GTCCCACCTAGGTTCAGTGGCAGTGGCTCTGGAACAGATTTCAGTCTCAC14820.11379910081817099No Hit
CCTTGGTCCCTGGGCCGAACGTCCGAGGATATCCGTTAGACTGTTGACAATAGTACGTTGCAAAATCCTCAGG14730.1131080131613805No Hit
CTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAGATGACCCAGTCTCCATCCTCTGTGTCTGCGTCTGT14710.11295443812653816No Hit
GTTCCAGAGCCACTGCCACTGAACCTAGGTGGGACCCCACCTTGCAAATTGGTTGCAGCATAGATCAGGGGCCT14590.11203298791748414No Hit
CTCTGGAACAGATTTCAGTCTCACAATCAGCAACCTCCAGCCTGAGGATTTTGCAACGTACTATTGTCAACAGT14580.11195620040006296No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14560.11180262536522062No Hit
GTATTAATAGACTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCTACAGACGCAGACACAGAGGATGG14430.11080438763874545No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14300.10980614991227027No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14220.10919184977290092No Hit
GAACCTAGGTGGGACCCCACCTTGCAAATTGGTTGCAGCATAGATCAGGGGCCTAGGGGCTTTCCCAGGTTTGTG14180.10888469970321624No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14170.10880791218579508No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14040.1078096744593199No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC13950.10711858680252939No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC13800.10596677404121185No Hit
GTATCAGCACAAACCTGGGAAAGCCCCTAGGCCCCTGATCTATGCTGCAACCAATTTGCAAGGTGGGGTCCCAC13750.10558283645410603No Hit
CTCTGTGTCTGCGTCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGTCTATTAATACCTGGTTAGC13690.10512211134957902No Hit
ATAGTACGTTGCAAAATCCTCAGGCTGGAGGTTGCTGATTGTGAGACTGAAATCTGTTCCAGAGCCACTGCC13650.10481496127989434No Hit
CACCATCACTTGTCGGGCGAGTCAGTCTATTAATACCTGGTTAGCCTGGTATCAGCACAAACCTGGGAAAGCCC13550.10404708610568267No Hit
ATGCTGCAACCAATTTGCAAGGTGGGGTCCCACCTAGGTTCAGTGGCAGTGGCTCTGGAACAGATTTCAGTCTC13410.10297206086178631No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGG13300.10212739817015346No Hit
GTCTCTGGTGGCTCCATCAGTAGTGGTGATTATTCCTGGAATTGGATCCG13260.10182024810046879No Hit
TCCGAGGATATCCGTTAGACTGTTGACAATAGTACGTTGCAAAATCCTCAGGCTGGAGGTTGCTGATTGTG13260.10182024810046879No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13220.10151309803078411No Hit
GCCTGGTATCAGCACAAACCTGGGAAAGCCCCTAGGCCCCTGATCTATGCTGCAACCAATTTGCAAGGTGGGGTC13200.10135952299594178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)